Abstract
Abstract Copper tolerance of Nicotiana tabacum L. var. Xanthi in vitro was achieved through plant regeneration from leaf explants on Murashige and Skoog's (MS) medium supplemented with 0.5 mg/l BA, 0.1–0.25 mg/l IAA and 60 μ m Cu. Tolerant organogenic calli showed more vigorous growth in medium containing 60 μ m Cu than the non‐tolerant calli. Standard growth parameters such as fresh and dry weight of organogenic callus, growth tolerance index (GTI), enzyme activity (peroxidase and catalase) and copper accumulation were used as indicators of copper tolerance. The activities of peroxidase and catalase as well as estimation of protein, total amino acid and chlorophyll were greater in tolerant calli than non‐tolerant ones. The GTI in the 4 weeks after the beginning of treatments yielded significant differences among the tolerant and non‐tolerant organogenic callus cultures. The accumulation of copper in the tolerant calli increased significantly with an increase in copper concentration in the medium. Shoot bud regeneration was achieved in both tolerant and non‐tolerant organogenic calli on MS medium containing 0.5 mg/l BA and 0.1 mg/l IAA. The tolerant regenerated shoots were rooted on half‐strength basal MS medium with 60 μ m Cu for selection of tolerant clones. This study may help in the selection and characterization of Cu‐tolerant lines of N. tabacum cv. ‘Xanthi’ for building conservation strategies and also for phytoremediation programmes.
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Claims 8
Claim · model-written summary
Copper-tolerant tobacco plants were regenerated from leaf explants on supplemented medium.
- Taxon
- Nicotiana tabacum L.
Nicotiana tabacume0 - Material
- Xanthie0
- Organ / stage
- leaf explantse0
- Treatment
- 60 μ m Cue0
- Outcome
- Copper tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Copper tolerance of Nicotiana tabacum L. var. Xanthi in vitro was achieved through plant regeneration from leaf explants on Murashige and Skoog's (MS) medium supplemented with 0.5 mg/l BA, 0.1–0.25 mg/l IAA and 60 μ m Cu”
Claim · model-written summary
Tolerant organogenic calli grew more vigorously than non-tolerant calli in copper medium.
- Taxon
- Nicotiana tabacum L.Nicotiana tabacumt0
- Material
- Tolerant organogenic callie0
- Treatment
- 60 μ m Cue0
- Outcome
- growthe0
- Direction
- more vigorouse0
- Comparator
- non‐tolerant callie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tolerant organogenic calli showed more vigorous growth in medium containing 60 μ m Cu than the non‐tolerant calli”
t0“Nicotiana tabacum L.”
Claim · model-written summary
Peroxidase and catalase activities were greater in tolerant than non-tolerant calli.
- Taxon
- Nicotiana tabacum L.Nicotiana tabacumt0
- Gene
- peroxidase and catalasee0
- Outcome
- activities of peroxidase and catalasee0
- Direction
- greatere0
- Comparator
- non‐tolerant onese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activities of peroxidase and catalase as well as estimation of protein, total amino acid and chlorophyll were greater in tolerant calli than non‐tolerant ones”
t0“Nicotiana tabacum L.”
Claim · model-written summary
Protein, total amino acid and chlorophyll estimates were greater in tolerant calli.
- Taxon
- Nicotiana tabacum L.Nicotiana tabacumt0
- Outcome
- protein, total amino acid and chlorophylle0
- Direction
- greatere0
- Comparator
- non‐tolerant onese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“estimation of protein, total amino acid and chlorophyll were greater in tolerant calli than non‐tolerant ones”
t0“Nicotiana tabacum L.”
Claim · model-written summary
Growth tolerance index differed significantly between tolerant and non-tolerant callus cultures after four weeks.
- Taxon
- Nicotiana tabacum L.Nicotiana tabacumt0
- Outcome
- GTIe0
- Qualifier
- 4 weeks after the beginning of treatmentse0
- Comparator
- non‐tolerant organogenic callus culturese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The GTI in the 4 weeks after the beginning of treatments yielded significant differences among the tolerant and non‐tolerant organogenic callus cultures”
t0“Nicotiana tabacum L.”
Claim · model-written summary
Copper accumulation in tolerant calli increased significantly as medium copper concentration increased.
- Taxon
- Nicotiana tabacum L.Nicotiana tabacumt0
- Treatment
- copper concentration in the mediume0
- Outcome
- accumulation of coppere0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The accumulation of copper in the tolerant calli increased significantly with an increase in copper concentration in the medium”
t0“Nicotiana tabacum L.”
Claim · model-written summary
Shoot buds regenerated in both tolerant and non-tolerant calli on supplemented medium.
- Taxon
- Nicotiana tabacum L.Nicotiana tabacumt0
- Organ / stage
- Shoot bude0
- Treatment
- MS medium containing 0.5 mg/l BA and 0.1 mg/l IAAe0
- Outcome
- Shoot bud regeneratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot bud regeneration was achieved in both tolerant and non‐tolerant organogenic calli on MS medium containing 0.5 mg/l BA and 0.1 mg/l IAA”
t0“Nicotiana tabacum L.”
Claim · model-written summary
Tolerant regenerated shoots rooted on half-strength copper-containing medium.
- Taxon
- Nicotiana tabacum L.Nicotiana tabacumt0
- Organ / stage
- regenerated shootse0
- Treatment
- half‐strength basal MS medium with 60 μ m Cue0
- Outcome
- rootede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The tolerant regenerated shoots were rooted on half‐strength basal MS medium with 60 μ m Cu”
t0“Nicotiana tabacum L.”
The same questions in other papers
copper atom · growth · Nicotiana tabacum (tobacco)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.