Abstract
BACKGROUND AND AIMS: Drought causes a decline of root hydraulic conductance, which aside from embolisms, is governed ultimately by aquaporins. Multiple factors probably regulate aquaporin expression, abundance and activity in leaf and root tissues during drought; among these are the leaf transpiration rate, leaf water status, abscisic acid (ABA) and soil water content. Here a study is made of how these factors could influence the response of aquaporin to drought. METHODS: Three plasma membrane intrinsic proteins (PIPs) or aquaporins were cloned from Phaseolus vulgaris plants and their expression was analysed after 4 d of water deprivation and also 1 d after re-watering. The effects of ABA and of methotrexate (MTX), an inhibitor of stomatal opening, on gene expression and protein abundance were also analysed. Protein abundance was examined using antibodies against PIP1 and PIP2 aquaporins. At the same time, root hydraulic conductance (L), transpiration rate, leaf water status and ABA tissue concentration were measured. KEY RESULTS: None of the treatments (drought, ABA or MTX) changed the leaf water status or tissue ABA concentration. The three treatments caused a decline in the transpiration rate and raised PVPIP2;1 gene expression and PIP1 protein abundance in the leaves. In the roots, only the drought treatment raised the expression of the three PIP genes examined, while at the same time diminishing PIP2 protein abundance and L. On the other hand, ABA raised both root PIP1 protein abundance and L. CONCLUSIONS: The rise of PvPIP2;1 gene expression and PIP1 protein abundance in the leaves of P. vulgaris plants subjected to drought was correlated with a decline in the transpiration rate. At the same time, the increase in the expression of the three PIP genes examined caused by drought and the decline of PIP2 protein abundance in the root tissues were not correlated with any of the parameters measured.
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Claims 8
Claim · model-written summary
Three plasma membrane intrinsic protein (PIP) aquaporin genes were cloned from Phaseolus vulgaris.
- Taxon
- Phaseolus vulgaris
Phaseolus vulgarise0 - Gene
- plasma membrane intrinsic proteins (PIPs)e0
- Outcome
- clonede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three plasma membrane intrinsic proteins (PIPs) or aquaporins were cloned from Phaseolus vulgaris plants”
Claim · model-written summary
Neither drought, ABA nor MTX changed leaf water status or tissue ABA concentration.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Treatment
- drought, ABA or MTXe1
- Outcome
- leaf water status or tissue ABA concentratione1
- Direction
- changede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three plasma membrane intrinsic proteins (PIPs) or aquaporins were cloned from Phaseolus vulgaris plants”
e1“None of the treatments (drought, ABA or MTX) changed the leaf water status or tissue ABA concentration”
Claim · model-written summary
The three treatments caused a decline in transpiration rate in P. vulgaris.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Treatment
- drought, ABA or MTXe1
- Outcome
- transpiration ratee2
- Direction
- declinee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three plasma membrane intrinsic proteins (PIPs) or aquaporins were cloned from Phaseolus vulgaris plants”
e1“None of the treatments (drought, ABA or MTX) changed the leaf water status or tissue ABA concentration”
e2“The three treatments caused a decline in the transpiration rate”
Claim · model-written summary
The three treatments raised PvPIP2;1 gene expression and PIP1 protein abundance in leaves.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Organ / stage
- leavese2
- Treatment
- drought, ABA or MTXe1
- Gene
- PVPIP2;1e2
- Outcome
- gene expression and PIP1 protein abundancee2
- Direction
- raisede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three plasma membrane intrinsic proteins (PIPs) or aquaporins were cloned from Phaseolus vulgaris plants”
e1“None of the treatments (drought, ABA or MTX) changed the leaf water status or tissue ABA concentration”
e2“raised PVPIP2;1 gene expression and PIP1 protein abundance in the leaves”
Claim · model-written summary
Drought alone raised the expression of the three PIP genes in roots.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Organ / stage
- rootse1
- Treatment
- droughte1
- Gene
- PIP genese1
- Outcome
- expression of the three PIP genese1
- Direction
- raisede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three plasma membrane intrinsic proteins (PIPs) or aquaporins were cloned from Phaseolus vulgaris plants”
e1“In the roots, only the drought treatment raised the expression of the three PIP genes examined”
Claim · model-written summary
Drought diminished PIP2 protein abundance and root hydraulic conductance (L) in roots.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Organ / stage
- rootse1
- Treatment
- droughte1
- Outcome
- PIP2 protein abundance and Le2
- Direction
- diminishinge2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three plasma membrane intrinsic proteins (PIPs) or aquaporins were cloned from Phaseolus vulgaris plants”
e1“In the roots, only the drought treatment raised the expression of the three PIP genes examined”
e2“while at the same time diminishing PIP2 protein abundance and L”
Claim · model-written summary
ABA raised root PIP1 protein abundance and root hydraulic conductance (L).
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise0
- Organ / stage
- roote1
- Treatment
- ABAe1
- Outcome
- PIP1 protein abundance and Le1
- Direction
- raisede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three plasma membrane intrinsic proteins (PIPs) or aquaporins were cloned from Phaseolus vulgaris plants”
e1“On the other hand, ABA raised both root PIP1 protein abundance and L”
Claim · model-written summary
The rise of PvPIP2;1 expression and PIP1 abundance in leaves of drought-subjected P. vulgaris was correlated with a decline in transpiration rate.
- Taxon
- P. vulgarisPhaseolus vulgarise0
- Organ / stage
- leavese0
- Treatment
- subjected to droughte0
- Gene
- PvPIP2;1e0
- Outcome
- transpiration ratee0
- Direction
- declinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rise of PvPIP2;1 gene expression and PIP1 protein abundance in the leaves of P. vulgaris plants subjected to drought was correlated with a decline in the transpiration rate”
The same questions in other papers
drought · physiology: water · Phaseolus vulgaris (common bean)Results differ
15 papers in the same direction as this paper · 8 in the opposite direction
- negative
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- positive
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