Abstract
myo-Inositol is a precursor for cell wall components, is used as a backbone of myo-inositol trisphosphate (Ins(1,4,5)P(3)) and phosphatidylinositol phosphate signaling molecules, and is debated about whether it is also a precursor in an alternate ascorbic acid synthesis pathway. Plants control inositol homeostasis by regulation of key enzymes involved in myo-inositol synthesis and catabolism. Recent transcriptional profiling data indicate up-regulation of the myo-inositol oxygenase (MIOX) genes under conditions in which energy or nutrients are limited. To test whether the MIOX genes are required for responses to low energy, we first examined MIOX2 and MIOX4 gene expression regulation by energy/nutrient conditions. We found that both MIOX2 and MIOX4 expression are suppressed by exogenous glucose addition in the shoot, but not in the root. Both genes were abundantly expressed during low energy/nutrient conditions. Loss-of-function mutants in MIOX genes contain alterations in myo-inositol levels and growth changes in the root. Miox2 mutants can be complemented with a MIOX2:green fluorescent protein fusion. Further we show here that MIOX2 is a cytoplasmic protein, while MIOX4 is present mostly in the cytoplasm, but also occasionally in the nucleus. Together, these data suggest that MIOX catabolism in the shoot may influence root growth responses during low energy/nutrient conditions.
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Claims 7
Claim · model-written summary
Exogenous glucose suppresses MIOX2 and MIOX4 expression in the shoot.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Organ / stage
- shoote1
- Treatment
- exogenous glucose additione1
- Gene
- MIOX2 and MIOX4e1
- Outcome
- MIOX2 and MIOX4 expressione1
- Direction
- suppressede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Arabidopsis thaliana”
e1“We found that both MIOX2 and MIOX4 expression are suppressed by exogenous glucose addition in the shoot, but not in the root”
Claim · model-written summary
Exogenous glucose does not suppress MIOX2 and MIOX4 expression in the root.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- roote1
- Treatment
- exogenous glucose additione1
- Gene
- MIOX2 and MIOX4e1
- Outcome
- MIOX2 and MIOX4 expressione1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Arabidopsis thaliana”
e1“We found that both MIOX2 and MIOX4 expression are suppressed by exogenous glucose addition in the shoot, but not in the root”
Claim · model-written summary
MIOX2 and MIOX4 are abundantly expressed under low energy and nutrient conditions.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- low energy/nutrient conditionse2
- Gene
- MIOX2 and MIOX4e1
- Outcome
- MIOX2 and MIOX4 expressione1
- Direction
- abundantly expressede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Arabidopsis thaliana”
e1“We found that both MIOX2 and MIOX4 expression are suppressed by exogenous glucose addition in the shoot, but not in the root”
e2“Both genes were abundantly expressed during low energy/nutrient conditions”
Claim · model-written summary
Loss-of-function MIOX mutants show altered myo-inositol levels.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- Loss-of-function mutants in MIOX genese3
- Outcome
- myo-inositol levelse3
- Direction
- alterationse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Arabidopsis thaliana”
e3“Loss-of-function mutants in MIOX genes contain alterations in myo-inositol levels and growth changes in the root”
Claim · model-written summary
Loss-of-function MIOX mutants show growth changes in the root.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- Loss-of-function mutants in MIOX genese3
- Organ / stage
- roote3
- Outcome
- growthe3
- Direction
- changese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Arabidopsis thaliana”
e3“Loss-of-function mutants in MIOX genes contain alterations in myo-inositol levels and growth changes in the root”
Claim · model-written summary
MIOX2 is a cytoplasmic protein.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- MIOX2e4
- Outcome
- cytoplasmic proteine4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Arabidopsis thaliana”
e4“MIOX2 is a cytoplasmic protein”
Claim · model-written summary
MIOX4 is present mostly in the cytoplasm and occasionally in the nucleus.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- MIOX4e5
- Outcome
- present mostly in the cytoplasme5
- Qualifier
- occasionally in the nucleuse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Arabidopsis thaliana”
e5“MIOX4 is present mostly in the cytoplasm, but also occasionally in the nucleus”
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glucose · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
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