Abstract
Abiotic stresses are major environmental factors that affect agricultural productivity worldwide. NAC transcription factors play pivotal roles in abiotic stress signaling in plants. As a staple crop, wheat production is severely constrained by abiotic stresses whereas only a few NAC transcription factors have been characterized functionally. To promote the application of NAC genes in wheat improvement by biotechnology, a novel NAC gene designated TaNAC67 was characterized in common wheat. To determine its role, transgenic Arabidopsis overexpressing TaNAC67-GFP controlled by the CaMV-35S promoter was generated and subjected to various abiotic stresses for morphological and physiological assays. Gene expression showed that TaNAC67 was involved in response to drought, salt, cold and ABA treatments. Localization assays revealed that TaNAC67 localized in the nucleus. Morphological analysis indicated the transgenics had enhanced tolerances to drought, salt and freezing stresses, simultaneously supported by enhanced expression of multiple abiotic stress responsive genes and improved physiological traits, including strengthened cell membrane stability, retention of higher chlorophyll contents and Na(+) efflux rates, improved photosynthetic potential, and enhanced water retention capability. Overexpression of TaNAC67 resulted in pronounced enhanced tolerances to drought, salt and freezing stresses, therefore it has potential for utilization in transgenic breeding to improve abiotic stress tolerance in crops.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
TaNAC67 expression responded to drought, salt, cold and ABA treatments.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- TaNAC67e0
- Outcome
- response to drought, salt, cold and ABA treatmentse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaNAC67 was involved in response to drought, salt, cold and ABA treatments.”
t0“Arabidopsis”
Claim · model-written summary
TaNAC67 localized in the nucleus.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- TaNAC67e0
- Outcome
- localized in the nucleuse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaNAC67 localized in the nucleus.”
t0“Arabidopsis”
Claim · model-written summary
TaNAC67 overexpressing Arabidopsis had enhanced tolerance to drought, salt and freezing.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- transgenicse0
- Gene
- TaNAC67t0
- Outcome
- tolerances to drought, salt and freezing stressese0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transgenics had enhanced tolerances to drought, salt and freezing stresses”
t0“TaNAC67 Confers Enhanced Multi-Abiotic Stress Tolerances in Arabidopsis”
Claim · model-written summary
TaNAC67 transgenics showed enhanced expression of multiple abiotic stress responsive genes.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- transgenicse0
- Outcome
- expression of multiple abiotic stress responsive genese0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transgenics had enhanced tolerances to drought, salt and freezing stresses, simultaneously supported by enhanced expression of multiple abiotic stress responsive genes”
t0“Arabidopsis”
Claim · model-written summary
TaNAC67 transgenics had strengthened cell membrane stability.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- transgenicse0
- Outcome
- cell membrane stabilitye0
- Direction
- strengthenede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transgenics had enhanced tolerances to drought, salt and freezing stresses, simultaneously supported by enhanced expression of multiple abiotic stress responsive genes and improved physiological traits, including strengthened cell membrane stability”
t0“Arabidopsis”
Claim · model-written summary
TaNAC67 transgenics retained higher chlorophyll contents and Na(+) efflux rates.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- transgenicse0
- Outcome
- chlorophyll contents and Na(+) efflux ratese0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transgenics had enhanced tolerances to drought, salt and freezing stresses, simultaneously supported by enhanced expression of multiple abiotic stress responsive genes and improved physiological traits, including strengthened cell membrane stability, retention of higher chlorophyll contents and Na(+) efflux rates”
t0“Arabidopsis”
Claim · model-written summary
TaNAC67 transgenics showed improved photosynthetic potential and water retention capability.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- transgenicse0
- Outcome
- photosynthetic potential, and enhanced water retention capabilitye0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transgenics had enhanced tolerances to drought, salt and freezing stresses, simultaneously supported by enhanced expression of multiple abiotic stress responsive genes and improved physiological traits, including strengthened cell membrane stability, retention of higher chlorophyll contents and Na(+) efflux rates, improved photosynthetic potential, and enhanced water retention capability”
t0“Arabidopsis”
Claim · model-written summary
The authors propose TaNAC67 for transgenic breeding of abiotic stress tolerant crops.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- utilization in transgenic breeding to improve abiotic stress tolerance in cropse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it has potential for utilization in transgenic breeding to improve abiotic stress tolerance in crops.”
t0“Arabidopsis”