Abstract
In a previous study, we isolated a new harpin protein, PopW, from the bacterium Ralstonia solanacearum ZJ3721 that can induce a hypersensitive response in tobacco, Nicotiana tabacum, leaves. In the current study, we demonstrate that, in a greenhouse experiment, PopW induced tobacco-acquired resistance against the Tobacco mosaic virus (TMV) with a biocontrol efficacy of 80.9 to 97.4% at a concentration as low as 25 μg/ml in both PopW-treated and neighboring leaves. The resistance induced by PopW is systemic acquired resistance mediated by salicylic acid, which was certified by the development of resistance being accompanied by the expression of the pathogenesis-related-1 gene (PR1) 8 h after PopW was sprayed onto the tobacco leaves. In addition, hydrogen peroxide began to accumulate 10 h after PopW spraying, peaking at 24 h with a maximum concentration of 1.97 μM/g fresh weight. The activities of phenylalanine ammonia lyase (EC4.3.1.5), polyphenoloxidase (EC1.14.18.1), and peroxidase (EC1.11.1.7) also increased, peaking at different times in the PopW-treated tobacco leaves. PopW also reduced the level of TMV disease in field trials with a biocontrol efficacy of 45.2%. Furthermore, PopW both increased tobacco yield (by 30.4 more than in control plants) and improved tobacco foliar quality, with an increase of 50.2% in the number of first-class tobacco leaves from treated compared with untreated plants. All of these results indicate that the new harpin protein PopW has the potential to be an effective biocontrol agent against TMV in tobacco.
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Claims 8
Claim · model-written summary
PopW induced tobacco resistance against TMV in greenhouse leaves, with 80.9 to 97.4% biocontrol efficacy.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- PopWe0
- Interactor
- Tobacco mosaic virus
Tobacco mosaic viruse0pathogen - Outcome
- tobacco-acquired resistance against the Tobacco mosaic virus (TMV)e0
- Direction
- inducede0
- Value
- 80.9 to 97.4%e0
- Setting
- greenhousee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in a greenhouse experiment, PopW induced tobacco-acquired resistance against the Tobacco mosaic virus (TMV) with a biocontrol efficacy of 80.9 to 97.4% at a concentration as low as 25 μg/ml in both PopW-treated and neighboring leaves.”
Claim · model-written summary
PopW-induced resistance was described as systemic acquired resistance mediated by salicylic acid.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- PopWe0
- Gene
- pathogenesis-related-1 gene (PR1)e0
- Compound
- salicylic acide0
- Outcome
- systemic acquired resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The resistance induced by PopW is systemic acquired resistance mediated by salicylic acid, which was certified by the development of resistance being accompanied by the expression of the pathogenesis-related-1 gene (PR1) 8 h after PopW was sprayed onto the tobacco leaves.”
Claim · model-written summary
Hydrogen peroxide accumulated after PopW spraying and peaked at 1.97 μM/g fresh weight after 24 h.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- PopW sprayinge0
- Outcome
- hydrogen peroxidee0
- Direction
- accumulatee0
- Value
- 1.97 μM/g fresh weighte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hydrogen peroxide began to accumulate 10 h after PopW spraying, peaking at 24 h with a maximum concentration of 1.97 μM/g fresh weight.”
t0“Resistance in Tobacco Under Greenhouse”
Claim · model-written summary
Phenylalanine ammonia lyase, polyphenoloxidase and peroxidase activities increased in PopW-treated tobacco leaves.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- PopW-treatede0
- Outcome
- activities of phenylalanine ammonia lyase (EC4.3.1.5), polyphenoloxidase (EC1.14.18.1), and peroxidase (EC1.11.1.7)e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activities of phenylalanine ammonia lyase (EC4.3.1.5), polyphenoloxidase (EC1.14.18.1), and peroxidase (EC1.11.1.7) also increased, peaking at different times in the PopW-treated tobacco leaves.”
Claim · model-written summary
PopW reduced TMV disease in field trials with 45.2% biocontrol efficacy.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- PopWe0
- Interactor
- TMVTobacco mosaic viruse0pathogen
- Outcome
- TMV diseasee0
- Direction
- reducede0
- Value
- 45.2%e0
- Setting
- field trialse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PopW also reduced the level of TMV disease in field trials with a biocontrol efficacy of 45.2%.”
t0“Resistance in Tobacco Under Greenhouse”
Claim · model-written summary
PopW increased tobacco yield by the reported 30.4 relative to controls.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- PopWe0
- Outcome
- tobacco yielde0
- Direction
- increasede0
- Value
- 30.4e0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PopW both increased tobacco yield (by 30.4 more than in control plants) and improved tobacco foliar quality, with an increase of 50.2% in the number of first-class tobacco leaves from treated compared with untreated plants.”
Claim · model-written summary
PopW increased the number of first-class tobacco leaves by 50.2% versus untreated plants.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- PopWe0
- Outcome
- number of first-class tobacco leavese0
- Direction
- increasee0
- Value
- 50.2%e0
- Comparator
- untreated plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PopW both increased tobacco yield (by 30.4 more than in control plants) and improved tobacco foliar quality, with an increase of 50.2% in the number of first-class tobacco leaves from treated compared with untreated plants.”
Claim · model-written summary
The authors propose PopW as a potential effective biocontrol agent against TMV in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Interactor
- TMVTobacco mosaic viruse0pathogen
- Outcome
- effective biocontrol agent against TMVe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the new harpin protein PopW has the potential to be an effective biocontrol agent against TMV in tobacco.”
The same questions in other papers
application · physiology: oxidative · Nicotiana tabacum (tobacco)Positive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.