Abstract
Brassinosteroids (BRs) are essential for plant growth and development; however, their roles in the regulation of stomatal opening or closure remain obscure. Here, the mechanism underlying BR-induced stomatal movements is studied. The effects of 24-epibrassinolide (EBR) on the stomatal apertures of tomato (Solanum lycopersicum) were measured by light microscopy using epidermal strips of wild type (WT), the abscisic acid (ABA)-deficient notabilis (not) mutant, and plants silenced for SlBRI1, SlRBOH1 and SlGSH1. EBR induced stomatal opening within an appropriate range of concentrations, whereas high concentrations of EBR induced stomatal closure. EBR-induced stomatal movements were closely related to dynamic changes in H(2)O(2) and redox status in guard cells. The stomata of SlRBOH1-silenced plants showed a significant loss of sensitivity to EBR. However, ABA deficiency abolished EBR-induced stomatal closure but did not affect EBR-induced stomatal opening. Silencing of SlGSH1, the critical gene involved in glutathione biosynthesis, disrupted glutathione redox homeostasis and abolished EBR-induced stomatal opening. The results suggest that transient H(2)O(2) production is essential for poising the cellular redox status of glutathione, which plays an important role in BR-induced stomatal opening. However, a prolonged increase in H(2)O(2) facilitated ABA signalling and stomatal closure.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
24-epibrassinolide (EBR) induced stomatal opening in tomato within an appropriate range of concentrations.
- Taxon
- Solanum lycopersicum
Solanum lycopersicumt0 - Treatment
- EBRe0
- Outcome
- stomatal openinge0
- Direction
- inducede0
- Qualifier
- appropriate range of concentrationse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum lycopersicum”
e0“EBR induced stomatal opening within an appropriate range of concentrations”
Claim · model-written summary
High concentrations of EBR induced stomatal closure in tomato.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Treatment
- high concentrations of EBRe0
- Outcome
- stomatal closuree0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum lycopersicum”
e0“high concentrations of EBR induced stomatal closure”
Claim · model-written summary
EBR-induced stomatal movements were closely related to dynamic changes in H2O2 and redox status in guard cells.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Organ / stage
- guard cellse0
- Treatment
- EBRe0
- Compound
- H(2)O(2)e0
- Outcome
- stomatal movementse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum lycopersicum”
e0“EBR-induced stomatal movements were closely related to dynamic changes in H(2)O(2) and redox status in guard cells”
Claim · model-written summary
The stomata of SlRBOH1-silenced plants showed a significant loss of sensitivity to EBR.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Material
- SlRBOH1-silenced plantse0
- Treatment
- EBRe0
- Gene
- SlRBOH1e0
- Outcome
- sensitivity to EBRe0
- Direction
- losse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum lycopersicum”
e0“The stomata of SlRBOH1-silenced plants showed a significant loss of sensitivity to EBR”
Claim · model-written summary
ABA deficiency abolished EBR-induced stomatal closure.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Material
- notabilis (not) mutante1
- Treatment
- ABA deficiencye0
- Outcome
- EBR-induced stomatal closuree0
- Direction
- abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum lycopersicum”
e0“ABA deficiency abolished EBR-induced stomatal closure”
e1“the abscisic acid (ABA)-deficient notabilis (not) mutant”
Claim · model-written summary
ABA deficiency did not affect EBR-induced stomatal opening.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Material
- notabilis (not) mutante2
- Treatment
- ABA deficiencye1
- Outcome
- EBR-induced stomatal openinge0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum lycopersicum”
e0“did not affect EBR-induced stomatal opening”
e1“ABA deficiency abolished EBR-induced stomatal closure”
e2“the abscisic acid (ABA)-deficient notabilis (not) mutant”
Claim · model-written summary
Silencing of SlGSH1, a gene of glutathione biosynthesis, disrupted glutathione redox homeostasis.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Treatment
- Silencing of SlGSH1e0
- Gene
- SlGSH1e0
- Outcome
- glutathione redox homeostasise0
- Direction
- disruptede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum lycopersicum”
e0“Silencing of SlGSH1, the critical gene involved in glutathione biosynthesis, disrupted glutathione redox homeostasis”
Claim · model-written summary
Silencing of SlGSH1 abolished EBR-induced stomatal opening.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Treatment
- Silencing of SlGSH1e0
- Gene
- SlGSH1e0
- Outcome
- EBR-induced stomatal openinge1
- Direction
- abolishede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum lycopersicum”
e0“Silencing of SlGSH1”
e1“abolished EBR-induced stomatal opening”
Claim · model-written summary
Transient H2O2 production is essential for poising the cellular glutathione redox status, which plays an important role in BR-induced stomatal opening.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Compound
- H(2)O(2)e0
- Outcome
- BR-induced stomatal openinge0
- Qualifier
- poising the cellular redox status of glutathionee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum lycopersicum”
e0“transient H(2)O(2) production is essential for poising the cellular redox status of glutathione, which plays an important role in BR-induced stomatal opening”
Claim · model-written summary
A prolonged increase in H2O2 facilitated ABA signalling and stomatal closure.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Compound
- H(2)O(2)e0
- Outcome
- ABA signalling and stomatal closuree0
- Qualifier
- prolonged increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum lycopersicum”
e0“a prolonged increase in H(2)O(2) facilitated ABA signalling and stomatal closure”
The same questions in other papers
(+)-abscisic acid · physiology: gas exchange · Solanum lycopersicum (tomato)Positive and negative results
1 paper in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.