Abstract
Efficient plant regeneration system has been developed from the nodal segments of chrysanthemum (Chrysanthemum morifolium L). Nodal segments, after being sterilized with 1.0% mercuric chloride for three minutes, were inoculated in Murashige and Skoog (MS) media with varied concentrations of indole acetic acid (IAA), benzylaminopurine (BAP) and their combinations. Different parameters including shoot initiation percentage, average number of shoots per explant, length of shoots (cm), number of leaves per shoot and number of nodes per shoot were studied during the course of study. Intermediate level (0.3 mg/l) of IAA exceeded all the other concentrations of IAA by producing 80.0 % shoot initiation, an average of 4.0 shoots per explants, 5.1 cm long shoots, 11.3 leaves and 5.6 nodes per shoot, when used alone. Similarly, intermediate level of BAP (1.0 mg/l) showed its supremacy over all the other concentrations as it produced 100% shoot initiation, 4.9 shoots per explant, 5.8 cm long shoots, 13.4 leaves and 6.3 nodes per shoot, when used alone. When the combination of different concentrations of IAA and BAP were used, significant results regarding the regeneration of chrysanthemum plantlets were also achieved. MS media supplemented with lower concentrations of IAA (0.1 and 0.2 mg/l) along with intermediate levels of BAP (1.0 and 2.0 mg/l) had a favorable effect on the regeneration of chrysanthemum plantlets using nodal segments of chrysanthemum, as compared to other concentrations and combinations. Satisfactory rooting response was obtained in half strength MS media supplemented with 0.2 mg/l indole butyric acid (IBA), followed by 0.2 mg/l naphthalene acetic acid (NAA) and IAA, respectively.
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Claims 12
Claim · model-written summary
IAA at 0.3 mg/l alone gave 80.0% shoot initiation, higher than other IAA concentrations.
- Taxon
- chrysanthemum
.jpeg?width=60)
Chrysanthemume0 - Organ / stage
- nodal segmentse0
- Treatment
- Intermediate level (0.3 mg/l) of IAAe1
- Outcome
- shoot initiatione1
- Direction
- exceedede1
- Value
- 80.0 %e1
- Comparator
- all the other concentrations of IAAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpeg?width=500)
Verbatim
e0“Efficient plant regeneration system has been developed from the nodal segments of chrysanthemum (Chrysanthemum morifolium L)”
e1“Intermediate level (0.3 mg/l) of IAA exceeded all the other concentrations of IAA by producing 80.0 % shoot initiation, an average of 4.0 shoots per explants, 5.1 cm long shoots, 11.3 leaves and 5.6 nodes per shoot, when used alone”
Claim · model-written summary
IAA at 0.3 mg/l alone gave an average of 4.0 shoots per explant, higher than other IAA concentrations.
- Taxon
- chrysanthemumChrysanthemume0
- Organ / stage
- nodal segmentse0
- Treatment
- Intermediate level (0.3 mg/l) of IAAe1
- Outcome
- shoots per explantse1
- Direction
- exceedede1
- Value
- 4.0e1
- Comparator
- all the other concentrations of IAAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Efficient plant regeneration system has been developed from the nodal segments of chrysanthemum (Chrysanthemum morifolium L)”
e1“Intermediate level (0.3 mg/l) of IAA exceeded all the other concentrations of IAA by producing 80.0 % shoot initiation, an average of 4.0 shoots per explants, 5.1 cm long shoots, 11.3 leaves and 5.6 nodes per shoot, when used alone”
Claim · model-written summary
IAA at 0.3 mg/l alone gave shoots 5.1 cm long, higher than other IAA concentrations.
- Taxon
- chrysanthemumChrysanthemume0
- Organ / stage
- nodal segmentse0
- Treatment
- Intermediate level (0.3 mg/l) of IAAe1
- Outcome
- long shootse1
- Direction
- exceedede1
- Value
- 5.1 cme1
- Comparator
- all the other concentrations of IAAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Efficient plant regeneration system has been developed from the nodal segments of chrysanthemum (Chrysanthemum morifolium L)”
e1“Intermediate level (0.3 mg/l) of IAA exceeded all the other concentrations of IAA by producing 80.0 % shoot initiation, an average of 4.0 shoots per explants, 5.1 cm long shoots, 11.3 leaves and 5.6 nodes per shoot, when used alone”
Claim · model-written summary
IAA at 0.3 mg/l alone gave 11.3 leaves, higher than other IAA concentrations.
- Taxon
- chrysanthemumChrysanthemume0
- Organ / stage
- nodal segmentse0
- Treatment
- Intermediate level (0.3 mg/l) of IAAe1
- Outcome
- leavese1
- Direction
- exceedede1
- Value
- 11.3e1
- Comparator
- all the other concentrations of IAAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Efficient plant regeneration system has been developed from the nodal segments of chrysanthemum (Chrysanthemum morifolium L)”
e1“Intermediate level (0.3 mg/l) of IAA exceeded all the other concentrations of IAA by producing 80.0 % shoot initiation, an average of 4.0 shoots per explants, 5.1 cm long shoots, 11.3 leaves and 5.6 nodes per shoot, when used alone”
Claim · model-written summary
IAA at 0.3 mg/l alone gave 5.6 nodes per shoot, higher than other IAA concentrations.
- Taxon
- chrysanthemumChrysanthemume0
- Organ / stage
- nodal segmentse0
- Treatment
- Intermediate level (0.3 mg/l) of IAAe1
- Outcome
- nodes per shoote1
- Direction
- exceedede1
- Value
- 5.6e1
- Comparator
- all the other concentrations of IAAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Efficient plant regeneration system has been developed from the nodal segments of chrysanthemum (Chrysanthemum morifolium L)”
e1“Intermediate level (0.3 mg/l) of IAA exceeded all the other concentrations of IAA by producing 80.0 % shoot initiation, an average of 4.0 shoots per explants, 5.1 cm long shoots, 11.3 leaves and 5.6 nodes per shoot, when used alone”
Claim · model-written summary
BAP at 1.0 mg/l alone gave 100% shoot initiation, higher than other BAP concentrations.
- Taxon
- chrysanthemumChrysanthemume0
- Organ / stage
- nodal segmentse0
- Treatment
- intermediate level of BAP (1.0 mg/l)e1
- Outcome
- shoot initiatione1
- Direction
- supremacye1
- Value
- 100%e1
- Comparator
- all the other concentrationse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Efficient plant regeneration system has been developed from the nodal segments of chrysanthemum (Chrysanthemum morifolium L)”
e1“Similarly, intermediate level of BAP (1.0 mg/l) showed its supremacy over all the other concentrations as it produced 100% shoot initiation, 4.9 shoots per explant, 5.8 cm long shoots, 13.4 leaves and 6.3 nodes per shoot, when used alone”
Claim · model-written summary
BAP at 1.0 mg/l alone gave 4.9 shoots per explant, higher than other BAP concentrations.
- Taxon
- chrysanthemumChrysanthemume0
- Organ / stage
- nodal segmentse0
- Treatment
- intermediate level of BAP (1.0 mg/l)e1
- Outcome
- shoots per explante1
- Direction
- supremacye1
- Value
- 4.9e1
- Comparator
- all the other concentrationse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Efficient plant regeneration system has been developed from the nodal segments of chrysanthemum (Chrysanthemum morifolium L)”
e1“Similarly, intermediate level of BAP (1.0 mg/l) showed its supremacy over all the other concentrations as it produced 100% shoot initiation, 4.9 shoots per explant, 5.8 cm long shoots, 13.4 leaves and 6.3 nodes per shoot, when used alone”
Claim · model-written summary
BAP at 1.0 mg/l alone gave 5.8 cm long shoots, higher than other BAP concentrations.
- Taxon
- chrysanthemumChrysanthemume0
- Organ / stage
- nodal segmentse0
- Treatment
- intermediate level of BAP (1.0 mg/l)e1
- Outcome
- long shootse1
- Direction
- supremacye1
- Value
- 5.8 cme1
- Comparator
- all the other concentrationse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Efficient plant regeneration system has been developed from the nodal segments of chrysanthemum (Chrysanthemum morifolium L)”
e1“Similarly, intermediate level of BAP (1.0 mg/l) showed its supremacy over all the other concentrations as it produced 100% shoot initiation, 4.9 shoots per explant, 5.8 cm long shoots, 13.4 leaves and 6.3 nodes per shoot, when used alone”
Claim · model-written summary
BAP at 1.0 mg/l alone gave 13.4 leaves, higher than other BAP concentrations.
- Taxon
- chrysanthemumChrysanthemume0
- Organ / stage
- nodal segmentse0
- Treatment
- intermediate level of BAP (1.0 mg/l)e1
- Outcome
- leavese1
- Direction
- supremacye1
- Value
- 13.4e1
- Comparator
- all the other concentrationse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Efficient plant regeneration system has been developed from the nodal segments of chrysanthemum (Chrysanthemum morifolium L)”
e1“Similarly, intermediate level of BAP (1.0 mg/l) showed its supremacy over all the other concentrations as it produced 100% shoot initiation, 4.9 shoots per explant, 5.8 cm long shoots, 13.4 leaves and 6.3 nodes per shoot, when used alone”
Claim · model-written summary
BAP at 1.0 mg/l alone gave 6.3 nodes per shoot, higher than other BAP concentrations.
- Taxon
- chrysanthemumChrysanthemume0
- Organ / stage
- nodal segmentse0
- Treatment
- intermediate level of BAP (1.0 mg/l)e1
- Outcome
- nodes per shoote1
- Direction
- supremacye1
- Value
- 6.3e1
- Comparator
- all the other concentrationse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Efficient plant regeneration system has been developed from the nodal segments of chrysanthemum (Chrysanthemum morifolium L)”
e1“Similarly, intermediate level of BAP (1.0 mg/l) showed its supremacy over all the other concentrations as it produced 100% shoot initiation, 4.9 shoots per explant, 5.8 cm long shoots, 13.4 leaves and 6.3 nodes per shoot, when used alone”
Claim · model-written summary
Lower IAA (0.1 and 0.2 mg/l) with intermediate BAP (1.0 and 2.0 mg/l) had a favorable effect on regeneration compared with other concentrations and combinations.
- Taxon
- chrysanthemumChrysanthemume1
- Organ / stage
- nodal segmentse1
- Treatment
- lower concentrations of IAA (0.1 and 0.2 mg/l) along with intermediate levels of BAP (1.0 and 2.0 mg/l)e1
- Outcome
- regeneration of chrysanthemum plantletse1
- Direction
- favorable effecte1
- Comparator
- other concentrations and combinationse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Efficient plant regeneration system has been developed from the nodal segments of chrysanthemum (Chrysanthemum morifolium L)”
e1“MS media supplemented with lower concentrations of IAA (0.1 and 0.2 mg/l) along with intermediate levels of BAP (1.0 and 2.0 mg/l) had a favorable effect on the regeneration of chrysanthemum plantlets using nodal segments of chrysanthemum, as compared to other concentrations and combinations”
Claim · model-written summary
Half-strength MS with 0.2 mg/l IBA gave a satisfactory rooting response, followed by NAA and IAA.
- Taxon
- chrysanthemumChrysanthemume0
- Treatment
- 0.2 mg/l indole butyric acid (IBA)e1
- Outcome
- rooting responsee1
- Qualifier
- Satisfactorye1
- Comparator
- 0.2 mg/l naphthalene acetic acid (NAA) and IAAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Efficient plant regeneration system has been developed from the nodal segments of chrysanthemum (Chrysanthemum morifolium L)”
e1“Satisfactory rooting response was obtained in half strength MS media supplemented with 0.2 mg/l indole butyric acid (IBA), followed by 0.2 mg/l naphthalene acetic acid (NAA) and IAA, respectively”
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