Abstract
The Zn/Cd-transporting ATPase, HMA2, has N- and C-terminal domains that can bind Zn ions with high affinity. Mutant derivatives were generated to determine the significance of these domains to HMA2 function in planta. Mutant derivatives, with and without a C-terminal GFP tag, were expressed from the HMA2 promoter in transgenic hma2,hma4, Zn-deficient, plants to test for functionality. A deletion mutant lacking the C-terminal 244 amino acids rescued most of the hma2,hma4 Zn-deficiency phenotypes with the exception of embryo or seed development. Root-to-shoot Cd translocation was fully rescued. The GFP-tagged derivative was partially mis-localized in the root pericycle cells in which it was expressed. Deletion derivatives lacking the C-terminal 121 and 21 amino acids rescued all phenotypes and localized normally. N-terminal domain mutants localized normally but failed to complement the hma2,hma4 phenotypes. These observations suggest that the N-terminal domain of HMA2 is essential for function in planta while the C-terminal domain, although not essential for function, may contain a signal important for the subcellular localization of the protein.
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Claims 8
Claim · model-written summary
A deletion lacking the C-terminal 244 amino acids of HMA2 rescued most Zn-deficiency phenotypes of hma2,hma4 plants
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Treatment
- deletion mutant lacking the C-terminal 244 amino acidse0
- Gene
- hma2,hma4e0
- Outcome
- Zn-deficiency phenotypese0
- Direction
- rescued moste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Arabidopsis thaliana”
e0“A deletion mutant lacking the C-terminal 244 amino acids rescued most of the hma2,hma4 Zn-deficiency phenotypes with the exception of embryo or seed development”
Claim · model-written summary
The 244-amino-acid C-terminal deletion did not rescue embryo or seed development
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- deletion mutant lacking the C-terminal 244 amino acidse0
- Gene
- hma2,hma4e0
- Outcome
- embryo or seed developmente0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Arabidopsis thaliana”
e0“A deletion mutant lacking the C-terminal 244 amino acids rescued most of the hma2,hma4 Zn-deficiency phenotypes with the exception of embryo or seed development”
Claim · model-written summary
Root-to-shoot Cd translocation was fully rescued by the C-terminal 244-amino-acid deletion
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- deletion mutant lacking the C-terminal 244 amino acidse0
- Outcome
- Root-to-shoot Cd translocatione1
- Direction
- fully rescuede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Arabidopsis thaliana”
e0“A deletion mutant lacking the C-terminal 244 amino acids rescued most of the hma2,hma4 Zn-deficiency phenotypes”
e1“Root-to-shoot Cd translocation was fully rescued”
Claim · model-written summary
The GFP-tagged HMA2 derivative was partially mis-localized in root pericycle cells
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- root pericycle cellse2
- Treatment
- GFP-tagged derivativee2
- Outcome
- mis-localizede2
- Direction
- partiallye2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Arabidopsis thaliana”
e2“The GFP-tagged derivative was partially mis-localized in the root pericycle cells in which it was expressed”
Claim · model-written summary
Deletions lacking the C-terminal 121 and 21 amino acids rescued all phenotypes and localized normally
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Deletion derivatives lacking the C-terminal 121 and 21 amino acidse3
- Outcome
- phenotypese3
- Direction
- rescued alle3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Arabidopsis thaliana”
e3“Deletion derivatives lacking the C-terminal 121 and 21 amino acids rescued all phenotypes and localized normally”
Claim · model-written summary
N-terminal domain mutants localized normally
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- N-terminal domain mutantse4
- Outcome
- localized normallye4
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Arabidopsis thaliana”
e4“N-terminal domain mutants localized normally but failed to complement the hma2,hma4 phenotypes”
Claim · model-written summary
N-terminal domain mutants failed to complement the hma2,hma4 phenotypes
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- N-terminal domain mutantse4
- Outcome
- hma2,hma4 phenotypese4
- Direction
- failed to complemente4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Arabidopsis thaliana”
e4“N-terminal domain mutants localized normally but failed to complement the hma2,hma4 phenotypes”
Claim · model-written summary
The N-terminal domain of HMA2 is essential for function in planta
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- HMA2e5
- Outcome
- function in plantae5
- Qualifier
- essentiale5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in Arabidopsis thaliana”
e5“the N-terminal domain of HMA2 is essential for function in planta”
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