Abstract
Somatic embryogenesis from leaf midrib explants of Dendranthema grandiflora Tzvelev. `Iridon' cultured on modified Murashige and Skoog basal medium (MSB) containing 1.0 mg 2,4-D and 0.2 mg BA/liter was influenced by light and sucrose concentration. Somatic embryos formed directly from explants when cultured on medium containing 9% to 18% sucrose and incubated first in the dark for 28 days, followed by 10 days in light, and then returned to the dark for 14 days. Embryogenesis did not occur in continuous darkness and was drastically reduced when explants were incubated in light only. The most embryos were formed on medium containing either 12% or 15% sucrose; lower concentrations stimulated shoot and root development. Light also mediated embryogenesis from leaf explants of 'other cultivars. White-opaque or occasionally light-green cotyledon-stage somatic embryos germinated on MSB medium without growth regulators but containing 3% sucrose. Twelve of the 23 cultivars evaluated produced somatic embryos, but plants were recovered from only five. Regenerated plants were phenotypically similar to parent plants in growth habit, leaf morphology, and flower color. Chemical names used: N- (phenylmethyl)-1 H- purine-6-amine (BA); (2,4-dichlorophenoxy) acetic acid (2,4-D).
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Claims 10
Claim · model-written summary
Somatic embryos formed directly from explants under 9% to 18% sucrose with dark, light, then dark incubation.
- Taxon
- Dendranthema grandiflora
Chrysanthemum ×morifoliumt0 - Treatment
- 9% to 18% sucrosee0
- Outcome
- Somatic embryos formed directly from explantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Somatic embryos formed directly from explants when cultured on medium containing 9% to 18% sucrose and incubated first in the dark for 28 days, followed by 10 days in light, and then returned to the dark for 14 days”
t0“Dendranthema grandiflora”
Claim · model-written summary
Embryogenesis did not occur in continuous darkness.
- Taxon
- Dendranthema grandifloraChrysanthemum ×morifoliumt0
- Treatment
- continuous darknesse0
- Outcome
- Embryogenesis did not occure0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Embryogenesis did not occur in continuous darkness”
t0“Dendranthema grandiflora”
Claim · model-written summary
Light-only incubation drastically reduced embryogenesis.
- Taxon
- Dendranthema grandifloraChrysanthemum ×morifoliumt0
- Treatment
- light onlye0
- Outcome
- Embryogenesise0
- Direction
- drastically reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Embryogenesis did not occur in continuous darkness and was drastically reduced when explants were incubated in light only”
t0“Dendranthema grandiflora”
Claim · model-written summary
The most embryos formed at 12% or 15% sucrose.
- Taxon
- Dendranthema grandifloraChrysanthemum ×morifoliumt0
- Treatment
- 12% or 15% sucrosee0
- Outcome
- embryose0
- Direction
- moste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The most embryos were formed on medium containing either 12% or 15% sucrose”
t0“Dendranthema grandiflora”
Claim · model-written summary
Lower sucrose concentrations stimulated shoot and root development.
- Taxon
- Dendranthema grandifloraChrysanthemum ×morifoliumt0
- Treatment
- lower concentrationse0
- Outcome
- shoot and root developmente0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lower concentrations stimulated shoot and root development”
t0“Dendranthema grandiflora”
Claim · model-written summary
Light mediated embryogenesis from leaf explants of other cultivars.
- Taxon
- Dendranthema grandifloraChrysanthemum ×morifoliumt0
- Treatment
- Lighte0
- Outcome
- embryogenesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Light also mediated embryogenesis from leaf explants of 'other cultivars”
t0“Dendranthema grandiflora”
Claim · model-written summary
Somatic embryos germinated on regulator-free MSB medium with 3% sucrose.
- Taxon
- Dendranthema grandifloraChrysanthemum ×morifoliumt0
- Treatment
- MSB medium without growth regulators but containing 3% sucrosee0
- Outcome
- somatic embryos germinatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“White-opaque or occasionally light-green cotyledon-stage somatic embryos germinated on MSB medium without growth regulators but containing 3% sucrose”
t0“Dendranthema grandiflora”
Claim · model-written summary
Twelve of 23 cultivars produced somatic embryos.
- Taxon
- Dendranthema grandifloraChrysanthemum ×morifoliumt0
- Outcome
- produced somatic embryose0
- Value
- Twelve of the 23 cultivarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Twelve of the 23 cultivars evaluated produced somatic embryos”
t0“Dendranthema grandiflora”
Claim · model-written summary
Plants were recovered from only five cultivars.
- Taxon
- Dendranthema grandifloraChrysanthemum ×morifoliumt0
- Outcome
- plants were recoverede0
- Value
- fivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plants were recovered from only five”
t0“Dendranthema grandiflora”
Claim · model-written summary
Regenerated plants resembled parent plants in growth habit, leaf morphology and flower color.
- Taxon
- Dendranthema grandifloraChrysanthemum ×morifoliumt0
- Outcome
- phenotypically similar to parent plants in growth habit, leaf morphology, and flower colore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regenerated plants were phenotypically similar to parent plants in growth habit, leaf morphology, and flower color”
t0“Dendranthema grandiflora”