Abstract
Among the dramatic changes occurring during grape berry (Vitis vinifera) development, those affecting the flavonoid pathway have provoked a number of investigations in the last 10 years. In addition to producing several compounds involved in the protection of the berry and the dissemination of the seeds, final products of this pathway also play a critical role in berry and wine quality. In this article, we describe the cloning and functional characterization of VvMYB5b, a cDNA isolated from a grape berry (V. vinifera 'Cabernet Sauvignon') library. VvMYB5b encodes a protein belonging to the R2R3-MYB family of transcription factors and displays significant similarity with VvMYB5a, another MYB factor recently shown to regulate flavonoid synthesis in grapevine. The ability of VvMYB5a and VvMYB5b to activate the grapevine promoters of several structural genes of the flavonoid pathway was confirmed by transient expression of the corresponding cDNAs in grape cells. Overexpression of VvMYB5b in tobacco (Nicotiana tabacum) leads to an up-regulation of genes encoding enzymes of the flavonoid pathway and results in the accumulation of anthocyanin- and proanthocyanidin-derived compounds. The ability of VvMYB5b to regulate particularly the anthocyanin and the proanthocyanidin pathways is discussed in relation to other recently characterized MYB transcription factors in grapevine. Taken together, data presented in this article give insight into the transcriptional mechanisms associated with the regulation of the flavonoid pathway throughout grape berry development.
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Claims 5
Claim · model-written summary
VvMYB5b encodes an R2R3-MYB transcription factor similar to VvMYB5a.
- Taxon
- Vitis vinifera
Vitis viniferae1 - Gene
- VvMYB5be0
- Outcome
- protein belonging to the R2R3-MYB family of transcription factorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VvMYB5b encodes a protein belonging to the R2R3-MYB family of transcription factors and displays significant similarity with VvMYB5a”
e1“grape berry (Vitis vinifera)”
Claim · model-written summary
VvMYB5a activated promoters of several flavonoid pathway genes in grape cells.
- Treatment
- transient expression of the corresponding cDNAse0
- Gene
- VvMYB5ae0
- Outcome
- grapevine promoters of several structural genes of the flavonoid pathwaye0
- Direction
- activatee0
VitisNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ability of VvMYB5a and VvMYB5b to activate the grapevine promoters of several structural genes of the flavonoid pathway was confirmed by transient expression of the corresponding cDNAs in grape cells”
Claim · model-written summary
VvMYB5b activated promoters of several flavonoid pathway genes in grape cells.
- Treatment
- transient expression of the corresponding cDNAse0
- Gene
- VvMYB5be0
- Outcome
- grapevine promoters of several structural genes of the flavonoid pathwaye0
- Direction
- activatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ability of VvMYB5a and VvMYB5b to activate the grapevine promoters of several structural genes of the flavonoid pathway was confirmed by transient expression of the corresponding cDNAs in grape cells”
Claim · model-written summary
VvMYB5b overexpression upregulated flavonoid pathway enzyme genes in tobacco.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- Overexpression of VvMYB5be0
- Gene
- VvMYB5be0
- Outcome
- genes encoding enzymes of the flavonoid pathwaye0
- Direction
- up-regulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of VvMYB5b in tobacco (Nicotiana tabacum) leads to an up-regulation of genes encoding enzymes of the flavonoid pathway”
Claim · model-written summary
VvMYB5b overexpression caused accumulation of anthocyanin- and proanthocyanidin-derived compounds in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Overexpression of VvMYB5be0
- Gene
- VvMYB5be0
- Outcome
- accumulation of anthocyanin- and proanthocyanidin-derived compoundse0
- Direction
- accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of VvMYB5b in tobacco (Nicotiana tabacum) leads to an up-regulation of genes encoding enzymes of the flavonoid pathway and results in the accumulation of anthocyanin- and proanthocyanidin-derived compounds”
The same questions in other papers
transgenic · molecular: expression · VitisPositive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.