Abstract
We isolated two cDNA clones (OsCLC-1 and OsCLC-2) homologous to tobacco CLC-Nt1, which encodes a voltage-gated chloride channel, from rice (Oryza sativa L. ssp. japonica, cv. Nipponbare). The deduced amino acid sequences were highly conserved (87.9% identity with each other). Southern blot analysis of the rice genomic DNA revealed that OsCLC-1 and OsCLC-2 were single-copy genes on chromosomes 4 and 2, respectively. OsCLC-1 was expressed in most tissues, whereas OsCLC-2 was expressed only in the roots, nodes, internodes and leaf sheaths. The level of expression of OsCLC-1, but not of OsCLC-2, was increased by treatment with NaCl. Both genes could partly substitute for GEF1, which encodes the sole chloride channel in yeast, by restoring growth under ionic stress. These results indicate that both genes are chloride channel genes. The proteins from both genes were immunochemically detected in the tonoplast fraction. Tagged synthetic green fluorescent protein which was fused to OsCLC-1 or OsCLC-2 localized in the vacuolar membranes. These results indicate that the proteins may play a role in the transport of chloride ions across the vacuolar membrane. We isolated loss-of-function mutants of both genes from a panel of rice mutants produced by the insertion of a retrotransposon, Tos17, in the exon region, and found inhibition of growth at all life stages.
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Claims 12
Claim · model-written summary
OsCLC-1 and OsCLC-2 cDNA clones were isolated from rice.
- Taxon
- rice
Oryza sativae0 - Material
- Nipponbaree0
- Gene
- OsCLC-1 and OsCLC-2e0
- Outcome
- two cDNA clonese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We isolated two cDNA clones (OsCLC-1 and OsCLC-2) homologous to tobacco CLC-Nt1, which encodes a voltage-gated chloride channel, from rice (Oryza sativa L. ssp. japonica, cv. Nipponbare).”
Claim · model-written summary
The deduced OsCLC-1 and OsCLC-2 amino acid sequences shared 87.9% identity.
- Taxon
- RiceOryza sativat0
- Gene
- OsCLC-1 and OsCLC-2e1
- Outcome
- identitye0
- Value
- 87.9%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The deduced amino acid sequences were highly conserved (87.9% identity with each other).”
e1“two cDNA clones (OsCLC-1 and OsCLC-2)”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
OsCLC-1 was a single-copy gene on chromosome 4.
- Taxon
- RiceOryza sativat0
- Gene
- OsCLC-1e0
- Outcome
- single-copy genese0
- Value
- 4e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsCLC-1 and OsCLC-2 were single-copy genes on chromosomes 4 and 2, respectively.”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
OsCLC-2 was a single-copy gene on chromosome 2.
- Taxon
- RiceOryza sativat0
- Gene
- OsCLC-2e0
- Outcome
- single-copy genese0
- Value
- 2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsCLC-1 and OsCLC-2 were single-copy genes on chromosomes 4 and 2, respectively.”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
OsCLC-1 was expressed in most tissues.
- Taxon
- RiceOryza sativat0
- Gene
- OsCLC-1e0
- Outcome
- expressed in most tissuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsCLC-1 was expressed in most tissues”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
OsCLC-2 expression was confined to roots, nodes, internodes, and leaf sheaths.
- Taxon
- RiceOryza sativat0
- Organ / stage
- roots, nodes, internodes and leaf sheathse0
- Gene
- OsCLC-2e0
- Outcome
- expressed only in the roots, nodes, internodes and leaf sheathse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsCLC-2 was expressed only in the roots, nodes, internodes and leaf sheaths.”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
NaCl increased OsCLC-1 expression.
- Taxon
- RiceOryza sativat0
- Treatment
- NaCle0
- Gene
- OsCLC-1e0
- Outcome
- expression of OsCLC-1e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The level of expression of OsCLC-1, but not of OsCLC-2, was increased by treatment with NaCl.”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
NaCl did not increase OsCLC-2 expression.
- Taxon
- RiceOryza sativat0
- Treatment
- NaCle0
- Gene
- OsCLC-2e0
- Outcome
- level of expressione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The level of expression of OsCLC-1, but not of OsCLC-2, was increased by treatment with NaCl.”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
Both rice genes partially restored yeast growth under ionic stress when substituting for GEF1.
- Taxon
- RiceOryza sativat0
- Treatment
- Both genese0
- Gene
- GEF1e0
- Outcome
- restoring growthe0
- Direction
- restoringe0
- Qualifier
- under ionic stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both genes could partly substitute for GEF1, which encodes the sole chloride channel in yeast, by restoring growth under ionic stress.”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
Proteins from both genes were detected in the tonoplast fraction.
- Taxon
- RiceOryza sativat0
- Organ / stage
- tonoplast fractione0
- Outcome
- detected in the tonoplast fractione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The proteins from both genes were immunochemically detected in the tonoplast fraction.”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
OsCLC-1 and OsCLC-2 fusion proteins localized in vacuolar membranes.
- Taxon
- RiceOryza sativat0
- Organ / stage
- vacuolar membranese0
- Gene
- OsCLC-1 or OsCLC-2e0
- Outcome
- localized in the vacuolar membranese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tagged synthetic green fluorescent protein which was fused to OsCLC-1 or OsCLC-2 localized in the vacuolar membranes.”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
Loss-of-function mutants of both genes had inhibited growth at all life stages.
- Taxon
- riceOryza sativae0
- Material
- loss-of-function mutantse0
- Organ / stage
- all life stagese0
- Treatment
- insertion of a retrotransposon, Tos17e0
- Outcome
- growthe0
- Direction
- inhibitione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We isolated loss-of-function mutants of both genes from a panel of rice mutants produced by the insertion of a retrotransposon, Tos17, in the exon region, and found inhibition of growth at all life stages.”
The same questions in other papers
sodium chloride · molecular: expression · Oryza sativa (rice)Positive and negative results
3 papers in the same direction as this paper · 16 in the opposite direction
- positive
- positive
- positive
16 papers more in this group
See all 20 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.