Abstract
We previously demonstrated that amounts of Cat1 RNA in developing immature maize (Zea mays L.) embryos change in parallel with endogenous abscisic acid (ABA) content. In excised immature embryos, addition of ABA leads to a large increase in Cat1 RNA accumulation. The Cat1 transcript, however, also accumulates to high amounts in scutella of germinating embryos, where ABA content is low and decreasing. Here we show that application of ABA to germinated embryos no longer results in the up-regulation of the Cat1 transcript accumulation that is seen during embryogenesis. This suggests that factors other than ABA control Cat1 expression at this developmental stage. Using band-shift and southwestern analyses, we show that the change in sensitivity to ABA is paralleled by changes in nuclear proteins binding to a 28-bp region of the Cat1 promoter in vitro. One protein (CAT1BP-20) shows increased accumulation in the absence of ABA, suggesting that a repressor-mediated mechanism accounts for at least a portion of the ABA regulation of Cat1.
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Claims 4
Claim · model-written summary
ABA application did not upregulate Cat1 transcripts in germinated embryos.
- Organ / stage
- germinated embryose0
- Treatment
- ABAe0
- Gene
- Cat1e0
- Outcome
- Cat1 transcript accumulatione0
- Direction
- no longer results in the up-regulatione0
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“application of ABA to germinated embryos no longer results in the up-regulation of the Cat1 transcript accumulation”
t0“maize”
Claim · model-written summary
Nuclear protein binding to a Cat1 promoter region changed alongside ABA sensitivity.
- Treatment
- ABAe0
- Gene
- Cat1e0
- Outcome
- nuclear proteins binding to a 28-bp region of the Cat1 promotere0
- Setting
- in vitroe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the change in sensitivity to ABA is paralleled by changes in nuclear proteins binding to a 28-bp region of the Cat1 promoter in vitro”
t0“maize”
Claim · model-written summary
CAT1BP-20 accumulation increased without ABA.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One protein (CAT1BP-20) shows increased accumulation in the absence of ABA”
t0“maize”
Claim · model-written summary
The authors suggest a repressor-mediated contribution to ABA regulation of Cat1.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting that a repressor-mediated mechanism accounts for at least a portion of the ABA regulation of Cat1”
t0“maize”
The same questions in other papers
(+)-abscisic acid · molecular: expression · Zea mays (maize)Results differ
13 papers in the same direction as this paper · 37 in the opposite direction
- positive
- positive
- no effect
47 papers more in this group
See all 51 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.