Abstract
The phosphoinositide pathway and inositol-1,4,5-trisphosphate (InsP(3)) are implicated in plant responses to stress. To determine the downstream consequences of altered InsP(3)-mediated signaling, we generated transgenic Arabidopsis thaliana plants expressing the mammalian type I inositol polyphosphate 5-phosphatase (InsP 5-ptase), which specifically hydrolyzes soluble inositol phosphates and terminates the signal. Rapid transient Ca(2+) responses to a cold or salt stimulus were reduced by approximately 30% in these transgenic plants. Drought stress studies revealed, surprisingly, that the InsP 5-ptase plants lost less water and exhibited increased drought tolerance. The onset of the drought stress was delayed in the transgenic plants, and abscisic acid (ABA) levels increased less than in the wild-type plants. Stomatal bioassays showed that transgenic guard cells were less responsive to the inhibition of opening by ABA but showed an increased sensitivity to ABA-induced closure. Transcript profiling revealed that the drought-inducible ABA-independent transcription factor DREB2A and a subset of DREB2A-regulated genes were basally upregulated in the InsP 5-ptase plants, suggesting that InsP(3) is a negative regulator of these DREB2A-regulated genes. These results indicate that the drought tolerance of the InsP 5-ptase plants is mediated in part via a DREB2A-dependent pathway and that constitutive dampening of the InsP(3) signal reveals unanticipated interconnections between signaling pathways.
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Claims 10
Claim · model-written summary
InsP 5-ptase plants lost less water under drought stress.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Material
- InsP 5-ptase plantse2
- Treatment
- drought stresse2
- Outcome
- watere2
- Direction
- lost lesse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we generated transgenic Arabidopsis thaliana plants expressing the mammalian type I inositol polyphosphate 5-phosphatase”
e2“Drought stress studies revealed, surprisingly, that the InsP 5-ptase plants lost less water and exhibited increased drought tolerance”
Claim · model-written summary
InsP 5-ptase plants had increased drought tolerance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- InsP 5-ptase plantse2
- Treatment
- drought stresse2
- Outcome
- drought tolerancee2
- Direction
- increasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we generated transgenic Arabidopsis thaliana plants expressing the mammalian type I inositol polyphosphate 5-phosphatase”
e2“Drought stress studies revealed, surprisingly, that the InsP 5-ptase plants lost less water and exhibited increased drought tolerance”
Claim · model-written summary
The onset of drought stress was delayed in the transgenic plants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- drought stresse3
- Outcome
- onset of the drought stresse3
- Direction
- delayede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we generated transgenic Arabidopsis thaliana plants expressing the mammalian type I inositol polyphosphate 5-phosphatase”
e3“The onset of the drought stress was delayed in the transgenic plants, and abscisic acid (ABA) levels increased less than in the wild-type plants”
Claim · model-written summary
ABA levels increased less under drought in the transgenic plants than in wild-type plants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- drought stresse3
- Outcome
- abscisic acid (ABA) levelse3
- Direction
- increased lesse3
- Comparator
- wild-type plantse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we generated transgenic Arabidopsis thaliana plants expressing the mammalian type I inositol polyphosphate 5-phosphatase”
e3“The onset of the drought stress was delayed in the transgenic plants, and abscisic acid (ABA) levels increased less than in the wild-type plants”
Claim · model-written summary
Transient Ca2+ responses to a cold or salt stimulus were reduced by approximately 30% in the transgenic plants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- a cold or salt stimuluse1
- Outcome
- Ca(2+) responsese1
- Direction
- reducede1
- Value
- approximately 30%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we generated transgenic Arabidopsis thaliana plants expressing the mammalian type I inositol polyphosphate 5-phosphatase”
e1“Rapid transient Ca(2+) responses to a cold or salt stimulus were reduced by approximately 30% in these transgenic plants”
Claim · model-written summary
Transgenic guard cells were less responsive to the inhibition of stomatal opening by ABA.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Organ / stage
- guard cellse4
- Treatment
- ABAe4
- Outcome
- less responsive to the inhibition of opening by ABAe4
- Direction
- lesse4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we generated transgenic Arabidopsis thaliana plants expressing the mammalian type I inositol polyphosphate 5-phosphatase”
e4“Stomatal bioassays showed that transgenic guard cells were less responsive to the inhibition of opening by ABA but showed an increased sensitivity to ABA-induced closure”
Claim · model-written summary
Transgenic guard cells showed increased sensitivity to ABA-induced closure.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Organ / stage
- guard cellse4
- Treatment
- ABAe4
- Outcome
- sensitivity to ABA-induced closuree4
- Direction
- increasede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we generated transgenic Arabidopsis thaliana plants expressing the mammalian type I inositol polyphosphate 5-phosphatase”
e4“Stomatal bioassays showed that transgenic guard cells were less responsive to the inhibition of opening by ABA but showed an increased sensitivity to ABA-induced closure”
Claim · model-written summary
DREB2A and a subset of DREB2A-regulated genes were basally upregulated in the InsP 5-ptase plants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- InsP 5-ptase plantse5
- Gene
- DREB2Ae5
- Outcome
- DREB2A and a subset of DREB2A-regulated genese5
- Direction
- upregulatede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we generated transgenic Arabidopsis thaliana plants expressing the mammalian type I inositol polyphosphate 5-phosphatase”
e5“the drought-inducible ABA-independent transcription factor DREB2A and a subset of DREB2A-regulated genes were basally upregulated in the InsP 5-ptase plants”
Claim · model-written summary
InsP3 is a negative regulator of DREB2A-regulated genes.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- DREB2Ae6
- Compound
- InsP(3)e6
- Outcome
- DREB2A-regulated genese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we generated transgenic Arabidopsis thaliana plants expressing the mammalian type I inositol polyphosphate 5-phosphatase”
e6“suggesting that InsP(3) is a negative regulator of these DREB2A-regulated genes”
Claim · model-written summary
The drought tolerance of the InsP 5-ptase plants is mediated in part via a DREB2A-dependent pathway.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- DREB2Ae7
- Outcome
- drought tolerancee7
- Qualifier
- mediated in part via a DREB2A-dependent pathwaye7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we generated transgenic Arabidopsis thaliana plants expressing the mammalian type I inositol polyphosphate 5-phosphatase”
e7“the drought tolerance of the InsP 5-ptase plants is mediated in part via a DREB2A-dependent pathway”
The same questions in other papers
drought · tolerance: abiotic · Arabidopsis thaliana (thale cress)Results agree
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- positive
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See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.