Abstract
Antisense- or RNAi-mediated suppression of the biosynthesis of nutritionally inferior storage proteins is a promising strategy for improving the amino acid profile of seeds. However, the potential pleiotropic effects of this on interconnected pathways and the agronomic quality traits need to be addressed. In the current study, a transcriptomic analysis of an antisense C-hordein line of barley was performed, using a grain-specific cDNA array. The C-hordein antisense line is characterized by marked changes in storage protein and amino acid profiles, while the seed weight is within the normal range and no external morphological irregularities were observed. The results of the transcriptome analysis showed excellent correlation with data on changes in the relative proportions of storage proteins and amino acid composition. The antisense line had a lower C-hordein level and down-regulated transcript encoding C-hordein. The production of the S-rich B/gamma- and D-hordeins was increased and significantly higher steady-state expression levels of the corresponding genes were observed. The increased synthesis of S-rich hordeins appeared to increase the demand for sulphur and the S-rich amino acids (cysteine and methionine), resulting in an up-regulation of key genes in the appropriate biosynthetic pathways. This study demonstrated the utility of the grain-specific cDNA microarray analysis to detect perturbations induced by antisense suppression of plant processes.
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Claims 11
Claim · model-written summary
The C-hordein antisense barley line shows marked changes in storage protein and amino acid profiles.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaret0 - Material
- C-hordein antisense linee1
- Gene
- C-hordeine1
- Outcome
- storage protein and amino acid profilese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“in the barley grain”
e1“The C-hordein antisense line is characterized by marked changes in storage protein and amino acid profiles”
Claim · model-written summary
Seed weight of the C-hordein antisense barley line is within the normal range.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- seed weighte2
- Qualifier
- within the normal rangee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in the barley grain”
e2“the seed weight is within the normal range”
Claim · model-written summary
The C-hordein antisense barley line shows no external morphological irregularities.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- external morphological irregularitiese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in the barley grain”
e3“no external morphological irregularities were observed”
Claim · model-written summary
Transcriptome analysis of the C-hordein antisense barley line correlates excellently with changes in storage protein and amino acid composition.
- Taxon
- barleyHordeum vulgaret0
- Material
- C-hordein antisense linee1
- Outcome
- relative proportions of storage proteins and amino acid compositione4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in the barley grain”
e1“The C-hordein antisense line is characterized by marked changes in storage protein and amino acid profiles”
e4“The results of the transcriptome analysis showed excellent correlation with data on changes in the relative proportions of storage proteins and amino acid composition”
Claim · model-written summary
The C-hordein antisense barley line has a lower C-hordein protein level.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- antisense linee5
- Gene
- C-hordeine5
- Outcome
- C-hordein levele5
- Direction
- lowere5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in the barley grain”
e5“The antisense line had a lower C-hordein level and down-regulated transcript encoding C-hordein”
Claim · model-written summary
The C-hordein antisense barley line has down-regulated transcript encoding C-hordein.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- antisense linee5
- Gene
- C-hordeine5
- Outcome
- transcript encoding C-hordeine5
- Direction
- down-regulatede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in the barley grain”
e5“The antisense line had a lower C-hordein level and down-regulated transcript encoding C-hordein”
Claim · model-written summary
Production of the S-rich B/gamma- and D-hordeins is increased in the barley grain.
- Taxon
- barleyHordeum vulgaret0
- Gene
- B/gamma- and D-hordeinse1
- Outcome
- production of the S-rich B/gamma- and D-hordeinse1
- Direction
- increasede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in the barley grain”
e1“The production of the S-rich B/gamma- and D-hordeins was increased”
Claim · model-written summary
Steady-state expression levels of the genes for the S-rich B/gamma- and D-hordeins are significantly higher in the barley grain.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- steady-state expression levels of the corresponding genese1
- Direction
- highere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in the barley grain”
e1“significantly higher steady-state expression levels of the corresponding genes were observed”
Claim · model-written summary
Increased synthesis of S-rich hordeins appears to increase the demand for sulphur and the S-rich amino acids cysteine and methionine in barley grain.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- demand for sulphur and the S-rich amino acids (cysteine and methionine)e7
- Qualifier
- The increased synthesis of S-rich hordeinse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in the barley grain”
e7“The increased synthesis of S-rich hordeins appeared to increase the demand for sulphur and the S-rich amino acids (cysteine and methionine), resulting in an up-regulation of key genes in the appropriate biosynthetic pathways”
Claim · model-written summary
Increased synthesis of S-rich hordeins appears to result in up-regulation of key genes in the appropriate biosynthetic pathways in barley grain.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- up-regulation of key genes in the appropriate biosynthetic pathwayse7
- Qualifier
- The increased synthesis of S-rich hordeinse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in the barley grain”
e7“The increased synthesis of S-rich hordeins appeared to increase the demand for sulphur and the S-rich amino acids (cysteine and methionine), resulting in an up-regulation of key genes in the appropriate biosynthetic pathways”
Claim · model-written summary
The grain-specific cDNA microarray analysis is useful for detecting perturbations induced by antisense suppression in barley.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- utility of the grain-specific cDNA microarray analysise1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in the barley grain”
e1“This study demonstrated the utility of the grain-specific cDNA microarray analysis to detect perturbations induced by antisense suppression of plant processes”
The same questions in other papers
loss of function · molecular: expression · Hordeum vulgare (barley)Positive and negative results
3 papers in the same direction as this paper · 9 in the opposite direction
- no effect
- negative
- negative
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.