Abstract
Lemon pistil explants were obtained by cutting just above the region of the hypogynous disc (A type explant) or at the base of the pistil (B type explant) and cultured on test medium containing Murashige and Skoog salts, 50 g sucrose/L, 100 mg myo-inositol/L, 5 mg thiamine–HCl/L, and 0.5 mg kinetin/L, plus or minus supplements. Under appropriate conditions an abscission zone formed and styles abscised after 6–8 days of culture; in the field stylar abscission occurred 12–15 days postanthesis. Abscission in A type explants was markedly inhibited by 9 μM 2,4-dichlorophenoxyacetic acid but was unaffected by indole-3-acetic, 1-naphthaleneacetic, gibberellic, abscisic, caffeic, or p-coumaric acids. The response to 2,4-dichlorophenoxyacetic acid was reduced in B type explants. In an atmosphere containing 35–200 ppm ethylene, cell division occurred in the zone of stylar abscission producing a proliferating callus, and the content of cellulase increased from 0.6 to 53.7 enzyme units/g fresh weight compared with fresh explants. Stylar abscission was inhibited by 2,4-dichlorophenoxyacetic acid in A type explants of Washington navel orange, Valencia orange, and mandarin pistils, but not of grapefruit pistils. B type explants of Washington navel orange and mandarin pistils were less responsive to 2,4-dichlorophenoxyacetic acid.
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Claims 10
Claim · model-written summary
Cultured lemon styles abscised after 6–8 days under suitable conditions.
- Taxon
- Lemon
Citrus ×limont0 - Organ / stage
- stylese0
- Outcome
- styles abscisede0
- Value
- 6–8 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under appropriate conditions an abscission zone formed and styles abscised after 6–8 days of culture”
t0“Lemon pistil explants”
Claim · model-written summary
Field stylar abscission occurred 12–15 days postanthesis.
CitrusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the field stylar abscission occurred 12–15 days postanthesis”
t0“Citrus”
Claim · model-written summary
2,4-dichlorophenoxyacetic acid markedly inhibited abscission in A type lemon explants.
- Taxon
- LemonCitrus ×limont0
- Material
- A type explantse0
- Treatment
- 9 μM 2,4-dichlorophenoxyacetic acide0
- Outcome
- Abscissione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Abscission in A type explants was markedly inhibited by 9 μM 2,4-dichlorophenoxyacetic acid”
t0“Lemon pistil explants”
Claim · model-written summary
Other tested acids did not affect abscission in A type explants.
- Taxon
- LemonCitrus ×limont1
- Treatment
- indole-3-acetic, 1-naphthaleneacetic, gibberellic, abscisic, caffeic, or p-coumaric acidse0
- Outcome
- Abscissiont0
- Direction
- unaffectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was unaffected by indole-3-acetic, 1-naphthaleneacetic, gibberellic, abscisic, caffeic, or p-coumaric acids”
t0“Abscission in A type explants”
t1“Lemon pistil explants”
Claim · model-written summary
The 2,4-dichlorophenoxyacetic acid response was reduced in B type explants.
- Taxon
- LemonCitrus ×limont0
- Material
- B type explantse0
- Outcome
- response to 2,4-dichlorophenoxyacetic acide0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The response to 2,4-dichlorophenoxyacetic acid was reduced in B type explants”
t0“Lemon pistil explants”
Claim · model-written summary
Ethylene induced cell division and proliferating callus in the stylar abscission zone.
- Organ / stage
- zone of stylar abscissione0
- Treatment
- 35–200 ppm ethylenee0
- Outcome
- cell division occurred in the zone of stylar abscission producing a proliferating calluse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In an atmosphere containing 35–200 ppm ethylene, cell division occurred in the zone of stylar abscission producing a proliferating callus”
t0“Citrus”
Claim · model-written summary
Under ethylene, cellulase content rose from 0.6 to 53.7 enzyme units/g fresh weight versus fresh explants.
- Treatment
- 35–200 ppm ethylenet0
- Gene
- cellulasee0
- Outcome
- content of cellulasee0
- Direction
- increasede0
- Value
- 53.7 enzyme units/g fresh weighte0
- Comparator
- fresh explantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the content of cellulase increased from 0.6 to 53.7 enzyme units/g fresh weight compared with fresh explants”
t0“35–200 ppm ethylene”
t1“Citrus”
Claim · model-written summary
2,4-dichlorophenoxyacetic acid inhibited abscission in A type Washington navel orange, Valencia orange and mandarin pistils.
- Taxon
- Washington navel orange, Valencia orange, and mandarine0
- Material
- A type explantse0
- Treatment
- 2,4-dichlorophenoxyacetic acide0
- Outcome
- Stylar abscissione0
- Direction
- inhibitede0
Not reportedComparator
Verbatim
e0“Stylar abscission was inhibited by 2,4-dichlorophenoxyacetic acid in A type explants of Washington navel orange, Valencia orange, and mandarin pistils”
Claim · model-written summary
Grapefruit A type pistils did not show abscission inhibition by 2,4-dichlorophenoxyacetic acid.
- Taxon
- grapefruit
_Pompelino_Grapefruit_002.jpg?width=60)
Citrus ×paradisie0 - Material
- A type explantse1
- Treatment
- 2,4-dichlorophenoxyacetic acide1
- Outcome
- Stylar abscissione1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Pompelino_Grapefruit_002.jpg?width=500)
Verbatim
e0“but not of grapefruit pistils”
e1“Stylar abscission was inhibited by 2,4-dichlorophenoxyacetic acid in A type explants of Washington navel orange, Valencia orange, and mandarin pistils”
Claim · model-written summary
B type Washington navel orange and mandarin explants were less responsive to 2,4-dichlorophenoxyacetic acid.
Verbatim
e0“B type explants of Washington navel orange and mandarin pistils were less responsive to 2,4-dichlorophenoxyacetic acid”
The same questions in other papers
2,4-D · yield · Citrus x limon (lemon)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
2,4-D · yield · Citrus x paradisi (grapefruit)Positive and negative results
4 in the opposite direction
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.