Abstract
A blue light (cryptochrome) photoreceptor from Arabidopsis, cry1, has been identified recently and shown to mediate a number of blue light-dependent phenotypes. Similar to phytochrome, the cryptochrome photoreceptors are encoded by a gene family of homologous members with considerable amino acid sequence similarity within the N-terminal chromophore binding domain. The two members of the Arabidopsis cryptochrome gene family (CRY1 and CRY2) overlap in function, but their proteins differ in stability: cry2 is rapidly degraded under light fluences (green, blue, and UV) that activate the photoreceptor, but cry1 is not. Here, we demonstrate by overexpression in transgenic plants of cry1 and cry2 fusion constructs that their domains are functionally interchangeable. Hybrid receptor proteins mediate functions similar to cry1 and include inhibition of hypocotyl elongation and blue light-dependent anthocyanin accumulation; differences in activity appear to be correlated with differing protein stability. Because cry2 accumulates to high levels under low-light intensities, it may have greater significance in wild-type plants under conditions when light is limited.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Overexpressed cry1 and cry2 fusion constructs in transgenic Arabidopsis plants have functionally interchangeable domains.
- Taxon
- Arabidopsis
Arabidopsise0 - Material
- transgenic plantse1
- Treatment
- overexpression in transgenic plants of cry1 and cry2 fusion constructse1
- Outcome
- functionally interchangeablee1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A blue light (cryptochrome) photoreceptor from Arabidopsis, cry1”
e1“by overexpression in transgenic plants of cry1 and cry2 fusion constructs that their domains are functionally interchangeable”
Claim · model-written summary
Hybrid cry1/cry2 receptor proteins inhibited hypocotyl elongation in Arabidopsis, a function similar to cry1.
- Taxon
- ArabidopsisArabidopsise0
- Material
- Hybrid receptor proteinse2
- Treatment
- overexpression in transgenic plants of cry1 and cry2 fusion constructse1
- Outcome
- hypocotyl elongatione2
- Direction
- inhibitione2
- Comparator
- cry1e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A blue light (cryptochrome) photoreceptor from Arabidopsis, cry1”
e1“by overexpression in transgenic plants of cry1 and cry2 fusion constructs that their domains are functionally interchangeable”
e2“Hybrid receptor proteins mediate functions similar to cry1 and include inhibition of hypocotyl elongation”
Claim · model-written summary
Hybrid cry1/cry2 receptor proteins promoted blue light-dependent anthocyanin accumulation in Arabidopsis, a function similar to cry1.
- Taxon
- ArabidopsisArabidopsise0
- Material
- Hybrid receptor proteinse2
- Treatment
- overexpression in transgenic plants of cry1 and cry2 fusion constructse1
- Outcome
- anthocyanin accumulatione3
- Direction
- increaseinferrede3
- Comparator
- cry1e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A blue light (cryptochrome) photoreceptor from Arabidopsis, cry1”
e1“by overexpression in transgenic plants of cry1 and cry2 fusion constructs that their domains are functionally interchangeable”
e2“Hybrid receptor proteins mediate functions similar to cry1 and include inhibition of hypocotyl elongation”
e3“blue light-dependent anthocyanin accumulation”
Claim · model-written summary
Differences in activity of the hybrid proteins appear to be correlated with differing protein stability.
- Taxon
- ArabidopsisArabidopsise0
- Material
- Hybrid receptor proteinse2
- Outcome
- activitye4
- Qualifier
- protein stabilitye4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A blue light (cryptochrome) photoreceptor from Arabidopsis, cry1”
e2“Hybrid receptor proteins mediate functions similar to cry1 and include inhibition of hypocotyl elongation”
e4“differences in activity appear to be correlated with differing protein stability”
Claim · model-written summary
cry2 accumulates to high levels under low-light intensities in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- cry2 accumulates to high levelse5
- Qualifier
- under low-light intensitiese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A blue light (cryptochrome) photoreceptor from Arabidopsis, cry1”
e5“Because cry2 accumulates to high levels under low-light intensities”