Abstract
Herbaspirillum seropedicae is an endophytic diazotrophic bacterium, which associates with important agricultural plants. In the present study, we have investigated the attachment to and internal colonization of Phaseolus vulgaris roots by the H. seropedicae wild-type strain SMR1 and by a strain of H. seropedicae expressing a red fluorescent protein (DsRed) to track the bacterium in the plant tissues. Two-day-old P. vulgaris roots were incubated at 30°C for 15 min with 6 x 10(8) CFU/mL H. seropedicae SMR1 or RAM4. Three days after inoculation, 4 x 10(4) cells of endophytic H. seropedicae SMR1 were recovered per gram of fresh root, and 9 days after inoculation the number of endophytes increased to 4 x 10(6) CFU/g. The identity of the recovered bacteria was confirmed by amplification and sequencing of the 16SrRNA gene. Furthermore, confocal microscopy of P. vulgaris roots inoculated with H. seropedicae RAM4 showed that the bacterial cells were attached to the root surface 15 min after inoculation; fluorescent bacteria were visible in the internal tissues after 24 h and were found in the central cylinder after 72 h, showing that H. seropedicae RAM4 is capable of colonizing the roots of the dicotyledon P. vulgaris. Determination of dry weight of common bean inoculated with H. seropedicae SMR1 suggested that this bacterium has a negative effect on the growth of P. vulgaris.
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Claims 6
Claim · model-written summary
Three days after inoculation, 4 x 10(4) cells of endophytic H. seropedicae SMR1 were recovered per gram of fresh P. vulgaris root.
- Taxon
- Phaseolus vulgaris
Phaseolus vulgarise1 - Interactor
- H. seropedicae SMR1Herbaspirillum seropedicaee2symbiont
- Outcome
- endophytic H. seropedicae SMR1 were recovered per gram of fresh roote2
- Value
- 4 x 10(4) cellse2
- Qualifier
- Three days after inoculatione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“internal colonization of Phaseolus vulgaris roots by the H. seropedicae wild-type strain SMR1”
e2“Three days after inoculation, 4 x 10(4) cells of endophytic H. seropedicae SMR1 were recovered per gram of fresh root”
Claim · model-written summary
The number of endophytes of H. seropedicae increased to 4 x 10(6) CFU/g in P. vulgaris roots 9 days after inoculation.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise1
- Interactor
- H. seropedicaeHerbaspirillum seropedicaee1symbiont
- Outcome
- number of endophytese3
- Direction
- increasede3
- Value
- 4 x 10(6) CFU/ge3
- Qualifier
- 9 days after inoculatione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“internal colonization of Phaseolus vulgaris roots by the H. seropedicae wild-type strain SMR1”
e3“9 days after inoculation the number of endophytes increased to 4 x 10(6) CFU/g”
Claim · model-written summary
H. seropedicae RAM4 cells were attached to the root surface of P. vulgaris 15 min after inoculation.
- Taxon
- P. vulgarisPhaseolus vulgarise7
- Interactor
- H. seropedicae RAM4Herbaspirillum seropedicaee7symbiont
- Outcome
- attached to the root surfacee4
- Value
- 15 mine4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“the bacterial cells were attached to the root surface 15 min after inoculation”
e7“H. seropedicae RAM4 is capable of colonizing the roots of the dicotyledon P. vulgaris”
Claim · model-written summary
Fluorescent H. seropedicae RAM4 bacteria were visible in the internal tissues of P. vulgaris roots after 24 h.
- Taxon
- P. vulgarisPhaseolus vulgarise7
- Organ / stage
- internal tissuese5
- Interactor
- H. seropedicae RAM4Herbaspirillum seropedicaee7symbiont
- Outcome
- visible in the internal tissuese5
- Value
- 24 he5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“fluorescent bacteria were visible in the internal tissues after 24 h”
e7“H. seropedicae RAM4 is capable of colonizing the roots of the dicotyledon P. vulgaris”
Claim · model-written summary
H. seropedicae RAM4 bacteria were found in the central cylinder of P. vulgaris roots after 72 h.
- Taxon
- P. vulgarisPhaseolus vulgarise7
- Organ / stage
- central cylindere5
- Interactor
- H. seropedicae RAM4Herbaspirillum seropedicaee7symbiont
- Outcome
- found in the central cylindere5
- Value
- 72 he5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“fluorescent bacteria were visible in the internal tissues after 24 h and were found in the central cylinder after 72 h”
e7“H. seropedicae RAM4 is capable of colonizing the roots of the dicotyledon P. vulgaris”
Claim · model-written summary
Inoculation with H. seropedicae SMR1 suggested a negative effect on the dry weight of common bean.
- Taxon
- common beanPhaseolus vulgarise6
- Treatment
- inoculated with H. seropedicae SMR1e6
- Interactor
- H. seropedicae SMR1Herbaspirillum seropedicaee6symbiont
- Outcome
- dry weighte6
- Direction
- negative effecte6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Determination of dry weight of common bean inoculated with H. seropedicae SMR1 suggested that this bacterium has a negative effect on the growth of P. vulgaris”
The same questions in other papers
Herbaspirillum seropedicae · growth · Phaseolus vulgaris (common bean)Results agree
1 paper in the same direction as this paper
- negative
inoculation · growth · Phaseolus vulgaris (common bean)Results differ
19 papers in the same direction as this paper · 106 in the opposite direction
- positive
- positive
- positive
122 papers more in this group
See all 126 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.