Abstract
Fusarium oxysporum f. sp. fragariae is a fungal pathogen causing Fusarium wilt on strawberry. Polymerase chain reaction (PCR) primers that can discriminate F. oxysporum f. sp. fragariae from nonpathogenic F. oxysporum would greatly assist pathogen identification. In order to develop a molecular diagnostic tool for this pathogen, transposable elements in the pathogen were characterized and used for designing a specific set of PCR primers. Portions of the transposable elements Fot3, Han, Hop, Hornet1, and Skippy were detected in all 33 strains of F. oxysporum f. sp. fragariae tested by PCR, whereas Foxy was detected in 32 strains and Impala sequences were detected in 30 strains. Two types of sequences were detected for Hop, two types for Impala, and three types for Skippy. The genomic region between Han and Skippy was amplified by an inter-retrotransposon amplified polymorphism technique, and PCR primers (FofraF and FofraR) to specifically identify F. oxysporum f. sp. fragariae were designed from this region. The developed PCR primers discriminated F. oxysporum f. sp. fragariae strains from nonpathogenic F. oxysporum strains and five other formae speciales. Conidia of F. oxysporum f. sp. fragariae could be detected in brown lowland-type soil by PCR using the primers. After preculturing the soil sample on FoG2 medium, 1 × 10 2 conidia/g of soil could be detected; without preculturing, 1 × 10 3 conidia/g of soil were detected.
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Claims 10
Claim · model-written summary
Fot3, Han, Hop, Hornet1 and Skippy were detected in all 33 pathogen strains.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassae0 - Interactor
- F. oxysporum f. sp. fragariae
Fusarium oxysporum f. sp. fragariaee1pathogen - Outcome
- Fot3, Han, Hop, Hornet1, and Skippy were detectede1
- Value
- 33 strainse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“Fusarium wilt on strawberry”
e1“Fot3, Han, Hop, Hornet1, and Skippy were detected in all 33 strains of F. oxysporum f. sp. fragariae tested by PCR”
Claim · model-written summary
Foxy was detected in 32 pathogen strains.
- Taxon
- strawberryFragaria ×ananassae0
- Outcome
- Foxy was detectede1
- Value
- 32 strainse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fusarium wilt on strawberry”
e1“Foxy was detected in 32 strains”
Claim · model-written summary
Impala was detected in 30 pathogen strains.
- Taxon
- strawberryFragaria ×ananassae0
- Outcome
- Impala sequences were detectede1
- Value
- 30 strainse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fusarium wilt on strawberry”
e1“Impala sequences were detected in 30 strains”
Claim · model-written summary
Two Hop sequence types were detected.
- Taxon
- strawberryFragaria ×ananassae0
- Outcome
- types of sequences were detected for Hope1
- Value
- Two typese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fusarium wilt on strawberry”
e1“Two types of sequences were detected for Hop”
Claim · model-written summary
Two Impala sequence types were detected.
- Taxon
- strawberryFragaria ×ananassae0
- Outcome
- types for Impalae1
- Value
- two typese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fusarium wilt on strawberry”
e1“two types for Impala”
Claim · model-written summary
Three Skippy sequence types were detected.
- Taxon
- strawberryFragaria ×ananassae0
- Outcome
- types for Skippye1
- Value
- three typese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fusarium wilt on strawberry”
e1“three types for Skippy”
Claim · model-written summary
FofraF and FofraR primers were developed from the Han-Skippy region.
- Taxon
- strawberryFragaria ×ananassae0
- Interactor
- F. oxysporum f. sp. fragariaeFusarium oxysporum f. sp. fragariaee1pathogen
- Outcome
- PCR primers (FofraF and FofraR)e1
- Comparator
- F. oxysporum f. sp. fragariaee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fusarium wilt on strawberry”
e1“PCR primers (FofraF and FofraR) to specifically identify F. oxysporum f. sp. fragariae were designed from this region”
Claim · model-written summary
Developed primers discriminated pathogen strains from nonpathogenic strains and five formae speciales.
- Taxon
- strawberryFragaria ×ananassae0
- Interactor
- F. oxysporum f. sp. fragariaeFusarium oxysporum f. sp. fragariaee1pathogen
- Outcome
- discriminated F. oxysporum f. sp. fragariae strainse1
- Comparator
- nonpathogenic F. oxysporum strains and five other formae specialese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fusarium wilt on strawberry”
e1“The developed PCR primers discriminated F. oxysporum f. sp. fragariae strains from nonpathogenic F. oxysporum strains and five other formae speciales”
Claim · model-written summary
With preculture, primers detected 1 × 10 2 conidia/g of soil.
- Taxon
- strawberryFragaria ×ananassae0
- Outcome
- conidia/g of soil could be detectede1
- Value
- 1 × 10 2 conidia/g of soile1
- Qualifier
- After preculturing the soil sample on FoG2 mediume1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fusarium wilt on strawberry”
e1“After preculturing the soil sample on FoG2 medium, 1 × 10 2 conidia/g of soil could be detected”
Claim · model-written summary
Without preculture, primers detected 1 × 10 3 conidia/g of soil.
- Taxon
- strawberryFragaria ×ananassae0
- Outcome
- conidia/g of soil were detectede1
- Value
- 1 × 10 3 conidia/g of soile1
- Qualifier
- without preculturinge1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fusarium wilt on strawberry”
e1“without preculturing, 1 × 10 3 conidia/g of soil were detected”