Abstract
The ascomycete fungus Verticillium dahliae causes vascular wilt diseases in hundreds of dicotyledonous plant species. However, thus far, only few V. dahliae effectors have been identified, and regulators of pathogenicity remain unknown. In this study, we investigated the role of the V. dahliae homolog of Sge1, a transcriptional regulator that was previously implicated in pathogenicity and effector gene expression in Fusarium oxysporum. We show that V. dahliae Sge1 (VdSge1) is required for radial growth and production of asexual conidiospores, because VdSge1 deletion strains display reduced radial growth and reduced conidia production. Furthermore, we show that VdSge1 deletion strains have lost pathogenicity on tomato. Remarkably, VdSge1 is not required for induction of Ave1, the recently identified V. dahliae effector that activates resistance mediated by the Ve1 immune receptor in tomato. Further assessment of the role of VdSge1 in the induction of the nine most highly in-planta-induced genes that encode putative effectors revealed differential activity. Although the expression of one putative effector gene in addition to Ave1 was not affected by VdSge1 deletion, VdSge1 appeared to be required for the expression of six putative effector genes, whereas two of the putative effectors genes were found to be negatively regulated by VdSge1. In conclusion, our data suggest that VdSge1 differentially regulates V. dahliae effector gene expression.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
VdSge1 deletion reduced fungal radial growth.
- Taxon
- tomato
Solanum lycopersicume1 - Treatment
- VdSge1 deletion strainse0
- Interactor
- Verticillium dahliae
Verticillium dahliaet0pathogen - Gene
- VdSge1e0
- Outcome
- radial growthe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VdSge1 deletion strains display reduced radial growth and reduced conidia production”
e1“pathogenicity on tomato”
t0“Verticillium dahliae”
Claim · model-written summary
VdSge1 deletion reduced fungal conidia production.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- VdSge1 deletion strainse0
- Interactor
- Verticillium dahliaeVerticillium dahliaet0pathogen
- Gene
- VdSge1e0
- Outcome
- conidia productione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VdSge1 deletion strains display reduced radial growth and reduced conidia production”
e1“pathogenicity on tomato”
t0“Verticillium dahliae”
Claim · model-written summary
VdSge1 deletion strains lost pathogenicity on tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- VdSge1 deletion strainse0
- Gene
- VdSge1e0
- Outcome
- pathogenicity on tomatoe0
- Direction
- loste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VdSge1 deletion strains have lost pathogenicity on tomato”
Claim · model-written summary
VdSge1 was not required for Ave1 induction.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- VdSge1e0
- Outcome
- induction of Ave1e0
- Direction
- not requirede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VdSge1 is not required for induction of Ave1”
e1“pathogenicity on tomato”
Claim · model-written summary
Deletion did not affect expression of one additional putative effector gene.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- VdSge1 deletione0
- Gene
- VdSge1e0
- Outcome
- expression of one putative effector genee0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of one putative effector gene in addition to Ave1 was not affected by VdSge1 deletion”
e1“pathogenicity on tomato”
Claim · model-written summary
VdSge1 was required for expression of six putative effector genes.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- VdSge1e0
- Outcome
- expression of six putative effector genese0
- Direction
- requirede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VdSge1 appeared to be required for the expression of six putative effector genes”
e1“pathogenicity on tomato”
Claim · model-written summary
VdSge1 negatively regulated two putative effector genes.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- VdSge1e0
- Outcome
- two of the putative effectors genese0
- Direction
- negatively regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two of the putative effectors genes were found to be negatively regulated by VdSge1”
e1“pathogenicity on tomato”
The same questions in other papers
loss of function · disease · Solanum lycopersicum (tomato)Positive and negative results
62 papers in the same direction as this paper · 92 in the opposite direction
- negative
- negative
- no effect
151 papers more in this group
See all 155 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.