Abstract
Development of a reliable in vitro regeneration protocol is necessary to facilitate genetic transformation of faba bean. However, leaching of phenolics from the explants of most genotypes of faba bean to the culture medium causes browning, and eventually kills the explants, hindering in vitro regeneration. This study is aimed to minimize the effect of phenolics and to identify the most suitable types of explants for in vitro regeneration. We pre-treated faba bean seeds in polyvinylpyrrolidone (PVP), then cultured different types of explants on tissue culture media supplemented with an adsorbent (activated charcoal) and antioxidants (ascorbic acid, cysteine and silver nitrate). Our results showed that treating the over night soaked seed (after removing the seed coat) with PVP solution (1000 mg/l) for 1 h, followed by culturing in Murashige and Skoog medium (MS medium) with 3% (w/v) sucrose, 0.8% (w/v) agar, 2 mg/l 6-benzylaminopurine and 2 mg/l thidiazuron, supplemented with ascorbic acid (1 mg/l) or activated charcoal (10 g/l), greatly reduced lethal browning in explants and improved shoot regeneration. The shoots rooted on half-strength MS medium supplemented with 0.5 mg/l -naphthaleneacetic acid. The cotyledonary node is the most suitable type of explant for regeneration. Regenerated plantlets were successfully established in pots and set seeds in the green house.
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Claims 5
Claim · model-written summary
Ascorbic acid or activated charcoal in the medium greatly reduced lethal browning in faba bean explants after PVP seed pre-treatment.
- Taxon
- faba bean
Vicia fabat0 - Organ / stage
- explantse1
- Treatment
- ascorbic acid (1 mg/l) or activated charcoal (10 g/l)e1
- Outcome
- lethal browning in explantse1
- Direction
- reducede1
- Qualifier
- PVP solution (1000 mg/l)e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in vitro plantlet regeneration of faba bean”
e1“treating the over night soaked seed (after removing the seed coat) with PVP solution (1000 mg/l) for 1 h, followed by culturing in Murashige and Skoog medium (MS medium) with 3% (w/v) sucrose, 0.8% (w/v) agar, 2 mg/l 6-benzylaminopurine and 2 mg/l thidiazuron, supplemented with ascorbic acid (1 mg/l) or activated charcoal (10 g/l), greatly reduced lethal browning in explants and improved shoot regeneration”
Claim · model-written summary
Ascorbic acid or activated charcoal in the medium improved shoot regeneration of faba bean after PVP seed pre-treatment.
- Taxon
- faba beanVicia fabat0
- Treatment
- ascorbic acid (1 mg/l) or activated charcoal (10 g/l)e1
- Outcome
- shoot regeneratione1
- Direction
- improvede1
- Qualifier
- PVP solution (1000 mg/l)e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in vitro plantlet regeneration of faba bean”
e1“treating the over night soaked seed (after removing the seed coat) with PVP solution (1000 mg/l) for 1 h, followed by culturing in Murashige and Skoog medium (MS medium) with 3% (w/v) sucrose, 0.8% (w/v) agar, 2 mg/l 6-benzylaminopurine and 2 mg/l thidiazuron, supplemented with ascorbic acid (1 mg/l) or activated charcoal (10 g/l), greatly reduced lethal browning in explants and improved shoot regeneration”
Claim · model-written summary
The cotyledonary node is the most suitable faba bean explant type for regeneration.
- Taxon
- faba beanVicia fabat0
- Organ / stage
- cotyledonary nodee2
- Outcome
- regeneratione2
- Qualifier
- the most suitable type of explante2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in vitro plantlet regeneration of faba bean”
e2“The cotyledonary node is the most suitable type of explant for regeneration”
Claim · model-written summary
Regenerated faba bean plantlets were successfully established in pots and set seeds in the greenhouse.
- Taxon
- faba beanVicia fabat0
- Outcome
- successfully established in pots and set seedse3
- Setting
- green housee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in vitro plantlet regeneration of faba bean”
e3“Regenerated plantlets were successfully established in pots and set seeds in the green house”
Claim · model-written summary
Shoots of faba bean rooted on half-strength MS medium supplemented with 0.5 mg/l naphthaleneacetic acid.
- Taxon
- faba beanVicia fabat0
- Treatment
- half-strength MS medium supplemented with 0.5 mg/l -naphthaleneacetic acide4
- Outcome
- shoots rootede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in vitro plantlet regeneration of faba bean”
e4“The shoots rooted on half-strength MS medium supplemented with 0.5 mg/l -naphthaleneacetic acid”
The same questions in other papers
ascorbic acid · growth · Vicia faba (faba bean)Positive and negative results
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- negative
- positive
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See all 6 papers →charcoal · growth · Vicia faba (faba bean)Results agree
2 papers in the same direction as this paper
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ascorbic acid · disease · Vicia faba (faba bean)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.