Abstract
Abstract Polyphenoloxidase (EC 1.10.3.1) in eggplant ( Solatium melongena L) was purified by ammonium sulphate fractionation, DEAE‐Cellulofine and DEAE‐Toyopearl chromatography and Sephadex G‐100 gel filtration. The enzyme was purified about 110‐fold with a recovery of 5%. The purified enzyme more quickly oxidised chlorogenic acid (5‐caffeoylquinic acid, IUPAC) than 10 other substrates used. The K m value for the enzyme was found to be 0·50 mM with respect to chlorogenic acid; the optimum pH of the enzyme was about 4 with enzyme stability between pH 5 and 8. The enzyme was completely inactivated after heat treatment at 75°C for 30 min or 80°C for 5 min. Sodium metabisulphite, potassium cyanide and sodium fluoride markedly inhibited the enzyme activity.
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Claims 11
Claim · model-written summary
Eggplant polyphenoloxidase was purified about 110‐fold with 5% recovery.
- Taxon
- eggplant
Solanum melongenat0 - Gene
- polyphenoloxidaset0
- Outcome
- purifiede0
- Value
- 110‐folde0
- Qualifier
- 5%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The enzyme was purified about 110‐fold with a recovery of 5%.”
t0“polyphenoloxidase in eggplant (Solanum melongena)”
Claim · model-written summary
Eggplant polyphenoloxidase was recovered at 5%.
- Taxon
- eggplantSolanum melongenat0
- Gene
- polyphenoloxidaset0
- Outcome
- recoverye0
- Value
- 5%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The enzyme was purified about 110‐fold with a recovery of 5%.”
t0“polyphenoloxidase in eggplant (Solanum melongena)”
Claim · model-written summary
The enzyme oxidized chlorogenic acid more quickly than the 10 other tested substrates.
- Taxon
- eggplantSolanum melongenat0
- Gene
- polyphenoloxidaset0
- Compound
- chlorogenic acide0
- Outcome
- oxidised chlorogenic acide0
- Direction
- more quicklye0
- Comparator
- 10 other substratese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The purified enzyme more quickly oxidised chlorogenic acid (5‐caffeoylquinic acid, IUPAC) than 10 other substrates used.”
t0“polyphenoloxidase in eggplant (Solanum melongena)”
Claim · model-written summary
The enzyme K m for chlorogenic acid was 0·50 mM.
- Taxon
- eggplantSolanum melongenat0
- Gene
- polyphenoloxidaset0
- Compound
- chlorogenic acide0
- Outcome
- K m valuee0
- Value
- 0·50 mMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The K m value for the enzyme was found to be 0·50 mM with respect to chlorogenic acid”
t0“polyphenoloxidase in eggplant (Solanum melongena)”
Claim · model-written summary
The optimum pH of the enzyme was about 4.
- Taxon
- eggplantSolanum melongenat0
- Gene
- polyphenoloxidaset0
- Outcome
- optimum pHe0
- Value
- about 4e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the optimum pH of the enzyme was about 4”
t0“polyphenoloxidase in eggplant (Solanum melongena)”
Claim · model-written summary
The enzyme was stable between pH 5 and 8.
- Taxon
- eggplantSolanum melongenat0
- Gene
- polyphenoloxidaset0
- Outcome
- enzyme stabilitye0
- Value
- pH 5 and 8e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enzyme stability between pH 5 and 8”
t0“polyphenoloxidase in eggplant (Solanum melongena)”
Claim · model-written summary
Heating at 75°C for 30 minutes completely inactivated the enzyme.
- Taxon
- eggplantSolanum melongenat0
- Treatment
- heat treatment at 75°C for 30 mine0
- Gene
- polyphenoloxidaset0
- Outcome
- enzymee0
- Direction
- inactivatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The enzyme was completely inactivated after heat treatment at 75°C for 30 min or 80°C for 5 min.”
t0“polyphenoloxidase in eggplant (Solanum melongena)”
Claim · model-written summary
Heating at 80°C for 5 minutes completely inactivated the enzyme.
- Taxon
- eggplantSolanum melongenat0
- Treatment
- 80°C for 5 mine0
- Gene
- polyphenoloxidaset0
- Outcome
- enzymee0
- Direction
- inactivatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The enzyme was completely inactivated after heat treatment at 75°C for 30 min or 80°C for 5 min.”
t0“polyphenoloxidase in eggplant (Solanum melongena)”
Claim · model-written summary
Sodium metabisulphite markedly inhibited enzyme activity.
- Taxon
- eggplantSolanum melongenat0
- Treatment
- Sodium metabisulphitee0
- Gene
- polyphenoloxidaset0
- Outcome
- enzyme activitye0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sodium metabisulphite, potassium cyanide and sodium fluoride markedly inhibited the enzyme activity.”
t0“polyphenoloxidase in eggplant (Solanum melongena)”
Claim · model-written summary
Potassium cyanide markedly inhibited enzyme activity.
- Taxon
- eggplantSolanum melongenat0
- Treatment
- potassium cyanidee0
- Gene
- polyphenoloxidaset0
- Outcome
- enzyme activitye0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sodium metabisulphite, potassium cyanide and sodium fluoride markedly inhibited the enzyme activity.”
t0“polyphenoloxidase in eggplant (Solanum melongena)”
Claim · model-written summary
Sodium fluoride markedly inhibited enzyme activity.
- Taxon
- eggplantSolanum melongenat0
- Treatment
- sodium fluoridee0
- Gene
- polyphenoloxidaset0
- Outcome
- enzyme activitye0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sodium metabisulphite, potassium cyanide and sodium fluoride markedly inhibited the enzyme activity.”
t0“polyphenoloxidase in eggplant (Solanum melongena)”
The same questions in other papers
heat · physiology: enzyme activity · Solanum melongena (eggplant)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.