Abstract
Metal transport from the cytosol to the vacuole is thought to be an important component of ion tolerance and of a plant's potential for use in phytoremediation. The Arabidopsis antiporter CAX2 (calcium exchanger 2) may be a key mediator of this process. CAX2 expression in yeast suppressed both Ca(2+) and Mn(2+) growth defects. A peptide-specific antibody to the antiporter reacted with a 39-kD protein from plant vacuolar membranes. Tobacco (Nicotiana tabacum) plants expressing CAX2 accumulated more Ca(2+), Cd(2+), and Mn(2+) and were more tolerant to elevated Mn(2+) levels. Expression of CAX2 in tobacco increased Cd(2+) and Mn(2+) transport in isolated root tonoplast vesicles. These results suggest that CAX2 has a broad substrate range and modulation of this transporter may be an important component of future strategies to improve plant ion tolerance.
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Claims 6
Claim · model-written summary
CAX2 expression suppressed calcium and manganese growth defects in yeast.
- Taxon
- Tobacco
Nicotiana tabacumt0 - Gene
- CAX2e0
- Outcome
- growth defectse0
- Direction
- suppressede0
- Qualifier
- in yeaste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“CAX2 expression in yeast suppressed both Ca(2+) and Mn(2+) growth defects”
Claim · model-written summary
An antiporter antibody reacted with a 39-kD vacuolar membrane protein.
- Taxon
- TobaccoNicotiana tabacumt0
- Outcome
- reacted with a 39-kD protein from plant vacuolar membranese0
- Value
- 39-kDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“A peptide-specific antibody to the antiporter reacted with a 39-kD protein from plant vacuolar membranes”
Claim · model-written summary
CAX2 tobacco accumulated more calcium, cadmium and manganese.
- Taxon
- TobaccoNicotiana tabacume0
- Treatment
- expressing CAX2e0
- Gene
- CAX2e0
- Outcome
- Ca(2+), Cd(2+), and Mn(2+)e0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tobacco (Nicotiana tabacum) plants expressing CAX2 accumulated more Ca(2+), Cd(2+), and Mn(2+)”
Claim · model-written summary
CAX2 tobacco was more tolerant of elevated manganese.
- Taxon
- TobaccoNicotiana tabacume0
- Treatment
- expressing CAX2e0
- Gene
- CAX2e0
- Outcome
- tolerant to elevated Mn(2+) levelse0
- Direction
- more tolerante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tobacco (Nicotiana tabacum) plants expressing CAX2 accumulated more Ca(2+), Cd(2+), and Mn(2+) and were more tolerant to elevated Mn(2+) levels”
Claim · model-written summary
CAX2 expression raised cadmium and manganese transport in root tonoplast vesicles.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- root tonoplast vesiclese0
- Treatment
- Expression of CAX2e0
- Gene
- CAX2e0
- Outcome
- Cd(2+) and Mn(2+) transporte0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of CAX2 in tobacco increased Cd(2+) and Mn(2+) transport in isolated root tonoplast vesicles”
Claim · model-written summary
The results suggest CAX2 transports a broad range of substrates.
- Taxon
- TobaccoNicotiana tabacumt0
- Gene
- CAX2e0
- Outcome
- broad substrate rangee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“These results suggest that CAX2 has a broad substrate range”
The same questions in other papers
transgenic · nutrient: content · Nicotiana tabacum (tobacco)Positive and negative results
10 papers in the same direction as this paper · 3 in the opposite direction
- positive
- negative
- negative
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.