Abstract
Nonrace specific disease resistance 1 (NDR1) is a conserved downstream regulator of resistance (R) protein-derived signaling. We identified two NDR1-like sequences (GmNDR1a, b) from soybean, and investigated their roles in R-mediated resistance and pathogen effector detection. Silencing GmNDR1a and b in soybean shows that these genes are required for resistance derived from the Rpg1-b, Rpg3, and Rpg4 loci, against Pseudomonas syringae (Psg) expressing avrB, avrB2 and avrD1, respectively. Immunoprecipitation assays show that the GmNDR1 proteins interact with the AvrB2 and AvrD1 Psg effectors. This correlates with the enhanced virulence of Psg avrB2 and Psg avrD1 in GmNDR1-silenced rpg3 rpg4 plants, even though these strains are not normally more virulent on plants lacking cognate R loci. The GmNDR1 proteins interact with GmRIN4 proteins, but not with AvrB, or its cognate R protein Rpg1-b. However, the GmNDR1 proteins promote AvrB-independent activation of Rpg1-b when coexpressed with a phosphomimic derivative of GmRIN4b. The role of GmNDR1 proteins in Rpg1-b activation, their direct interactions with AvrB2/AvrD1, and a putative role in the virulence activities of Avr effectors, provides the first experimental evidence in support of the proposed role for NDR1 in transducing extracellular pathogen-derived signals.
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Claims 7
Claim · model-written summary
Two soybean NDR1-like sequences, GmNDR1a and b, were identified.
- Taxon
- soybean
Glycine maxe0 - Gene
- GmNDR1a, be0
- Outcome
- two NDR1-like sequencese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We identified two NDR1-like sequences (GmNDR1a, b) from soybean”
Claim · model-written summary
Silencing GmNDR1a and b showed their requirement for Rpg1-b, Rpg3 and Rpg4 mediated resistance to Psg strains.
- Taxon
- soybeanGlycine maxe0
- Treatment
- Silencing GmNDR1a and be0
- Interactor
- Pseudomonas syringae
Pseudomonas syringaee0pathogen - Gene
- GmNDR1a and be0
- Outcome
- resistance derived from the Rpg1-b, Rpg3, and Rpg4 locie0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing GmNDR1a and b in soybean shows that these genes are required for resistance derived from the Rpg1-b, Rpg3, and Rpg4 loci, against Pseudomonas syringae (Psg) expressing avrB, avrB2 and avrD1, respectively.”
Claim · model-written summary
GmNDR1 proteins interacted with AvrB2 and AvrD1 effectors.
- Taxon
- soybeanGlycine maxt0
- Interactor
- Psge0pathogen
- Gene
- GmNDR1 proteinse0
- Outcome
- interact with the AvrB2 and AvrD1 Psg effectorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the GmNDR1 proteins interact with the AvrB2 and AvrD1 Psg effectors.”
t0“Soybean”
Claim · model-written summary
Psg avrB2 and avrD1 had enhanced virulence in GmNDR1-silenced rpg3 rpg4 plants.
- Taxon
- soybeanGlycine maxt0
- Treatment
- GmNDR1-silenced rpg3 rpg4 plantse0
- Interactor
- Psge0pathogen
- Outcome
- virulence of Psg avrB2 and Psg avrD1e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhanced virulence of Psg avrB2 and Psg avrD1 in GmNDR1-silenced rpg3 rpg4 plants”
t0“Soybean”
Claim · model-written summary
GmNDR1 proteins interacted with GmRIN4 proteins.
- Taxon
- soybeanGlycine maxt0
- Gene
- GmNDR1 proteinse0
- Outcome
- interact with GmRIN4 proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The GmNDR1 proteins interact with GmRIN4 proteins”
t0“Soybean”
Claim · model-written summary
GmNDR1 proteins did not interact with AvrB or Rpg1-b.
- Taxon
- soybeanGlycine maxt0
- Gene
- Rpg1-be0
- Outcome
- not with AvrB, or its cognate R protein Rpg1-be0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not with AvrB, or its cognate R protein Rpg1-b.”
t0“Soybean”
Claim · model-written summary
GmNDR1 proteins promoted AvrB-independent Rpg1-b activation with phosphomimic GmRIN4b.
- Taxon
- soybeanGlycine maxt0
- Treatment
- coexpressed with a phosphomimic derivative of GmRIN4be0
- Gene
- GmNDR1 proteinse0
- Outcome
- activation of Rpg1-be0
- Direction
- promotee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the GmNDR1 proteins promote AvrB-independent activation of Rpg1-b when coexpressed with a phosphomimic derivative of GmRIN4b.”
t0“Soybean”
The same questions in other papers
loss of function · Pseudomonas syringae · Glycine max (soybean)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.