Abstract
The shiitake mushroom (Lentinula edodes) has been used in research involving the prevention and the control of human and plant diseases. In cucumber plants, treated with aqueous extracts from mushroom basidiocarps, there was a reduction in anthracnose severity caused by Colletotrichum lagenarium, and an increase in peroxidase activity in the leaves. With the aim of obtaining molecules of agronomic interest, the crude aqueous extract from L. edodes basidiocarp was fractioned with ammonium sulfate. The fraction corresponding to 40-80% of saturation (p40-80), the most effective in reducing anthracnose on cucumber cotyledons, was submitted to anion exchange chromatography (AEC). After AEC, six protein peaks were obtained and the peak V, containing 34% of the proteins present in p40-80, induced peroxidase increase in the cucumber cotyledons besides reducing anthracnose severity. Separation of peak V proteins by SDS-gel electrophoresis revealed the presence of more than one band in the gel. Thus, a partial purification of elicitors present in the L. edodes basidiocarp was achieved.
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Claims 9
Claim · model-written summary
Aqueous mushroom extract reduced cucumber anthracnose severity.
- Taxon
- cucumber
Cucumis sativuse0 - Treatment
- aqueous extracts from mushroom basidiocarpse0
- Interactor
- Colletotrichum lagenariume0pathogen
- Outcome
- anthracnose severitye0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In cucumber plants, treated with aqueous extracts from mushroom basidiocarps, there was a reduction in anthracnose severity caused by Colletotrichum lagenarium”
t0“Lentinula edodes basidiocarps”
Claim · model-written summary
Aqueous mushroom extract increased leaf peroxidase activity in cucumber.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- leavese0
- Treatment
- aqueous extracts from mushroom basidiocarpse0
- Gene
- peroxidasee0
- Outcome
- peroxidase activitye0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In cucumber plants, treated with aqueous extracts from mushroom basidiocarps, there was a reduction in anthracnose severity caused by Colletotrichum lagenarium, and an increase in peroxidase activity in the leaves.”
Claim · model-written summary
The p40-80 fraction was the most effective fraction for reducing cucumber anthracnose.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- cotyledonse0
- Treatment
- p40-80e0
- Outcome
- anthracnosee0
- Direction
- reducinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fraction corresponding to 40-80% of saturation (p40-80), the most effective in reducing anthracnose on cucumber cotyledons”
Claim · model-written summary
Anion exchange chromatography of p40-80 yielded six protein peaks.
- Taxon
- cucumberCucumis sativust0
- Treatment
- AECe0
- Outcome
- protein peakse0
- Value
- six protein peakse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After AEC, six protein peaks were obtained”
e1“The fraction corresponding to 40-80% of saturation (p40-80)”
t0“cucumber seedlings”
Claim · model-written summary
Peak V contained 34% of the proteins in p40-80.
- Taxon
- cucumberCucumis sativust0
- Material
- peak Ve0
- Outcome
- proteins present in p40-80e0
- Value
- 34%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“peak V, containing 34% of the proteins present in p40-80”
t0“cucumber seedlings”
Claim · model-written summary
Peak V induced peroxidase increase in cucumber cotyledons.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- cotyledonse0
- Treatment
- peak Ve0
- Gene
- peroxidasee0
- Outcome
- peroxidasee0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“peak V, containing 34% of the proteins present in p40-80, induced peroxidase increase in the cucumber cotyledons”
Claim · model-written summary
Peak V reduced cucumber anthracnose severity.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- peak Ve0
- Interactor
- Colletotrichum lagenariumt0pathogen
- Outcome
- anthracnose severitye0
- Direction
- reducinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“peak V, containing 34% of the proteins present in p40-80, induced peroxidase increase in the cucumber cotyledons besides reducing anthracnose severity”
t0“Colletotrichum lagenarium”
Claim · model-written summary
SDS gel electrophoresis showed more than one protein band in peak V.
- Taxon
- cucumberCucumis sativust0
- Material
- peak Ve0
- Outcome
- more than one band in the gele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Separation of peak V proteins by SDS-gel electrophoresis revealed the presence of more than one band in the gel.”
t0“cucumber seedlings”
Claim · model-written summary
The study achieved partial purification of elicitors from L. edodes basidiocarps.
- Taxon
- cucumberCucumis sativust0
- Outcome
- partial purification of elicitorse0
- Value
- partiale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a partial purification of elicitors present in the L. edodes basidiocarp was achieved”
t0“cucumber seedlings”
The same questions in other papers
botanical · physiology: antioxidant enzymes · Cucumis sativus (cucumber)Positive and negative results
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- no effect
- positive
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See all 14 papers →botanical · disease · Cucumis sativus (cucumber)Positive and negative results
48 papers in the same direction as this paper · 8 in the opposite direction
- no effect
- negative
- negative
53 papers more in this group
See all 57 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.