Abstract
Abstract The addition of exogenous proline (10 mm) to Na100-saline culture medium, modified LS medium (Linsmaier and Skoog 1965: Physiol. Plant., 18, 100–127) with 100 mm NaCl promoted the growth of tobacco (Nicotiana tabacum L., cv. Bright Yellow-2) suspension cells unadapted to salt stress without maintaining a high ratio of K+ to Na+ ions under salinity conditions. The addition of exogenous glutamic acid or alanine were not comparable to that of exogenous proline. The proline contents of the NaCl-unadapted cells became much higher when the cells were grown in Na100-saline culture medium with 10 mm proline than when the cells were cultured without proline. The accumulation of K+, Na+, counter ions was sufficient to compensate for the increase of the water potential of the cells caused by salinity. These results suggest that exogenous proline does not act as a nitrogen store and that proline may act as a protectant for enzymes and membranes against salt inactivation rather than as a compatible solute in tobacco suspension cells.
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Claims 7
Claim · model-written summary
Adding 10 mm exogenous proline to Na100-saline culture medium promoted growth of tobacco Bright Yellow-2 suspension cells unadapted to salt stress.
- Taxon
- tobacco
Nicotiana tabacume2 - Material
- Bright Yellow-2e2
- Organ / stage
- suspension cellse2
- Treatment
- exogenous proline (10 mm)e0
- Outcome
- growthe2
- Direction
- promotede2
- Qualifier
- unadapted to salt stresse2
- Setting
- Na100-saline culture mediume1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of exogenous proline (10 mm)”
e1“to Na100-saline culture medium”
e2“promoted the growth of tobacco (Nicotiana tabacum L., cv. Bright Yellow-2) suspension cells unadapted to salt stress”
Claim · model-written summary
Exogenous proline promoted growth without maintaining a high K+ to Na+ ratio under salinity in tobacco cells.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- exogenous proline (10 mm)e0
- Outcome
- ratio of K+ to Na+ ionse2
- Direction
- without maintaininge2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of exogenous proline (10 mm)”
e1“tobacco (Nicotiana tabacum L., cv. Bright Yellow-2)”
e2“without maintaining a high ratio of K+ to Na+ ions under salinity conditions”
Claim · model-written summary
Exogenous glutamic acid or alanine were not comparable to exogenous proline in their effect on tobacco cells under salinity.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- exogenous glutamic acid or alaninee1
- Outcome
- not comparablee1
- Direction
- not comparablee1
- Comparator
- exogenous prolinee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco (Nicotiana tabacum L., cv. Bright Yellow-2)”
e1“The addition of exogenous glutamic acid or alanine were not comparable to that of exogenous proline”
Claim · model-written summary
Proline content of NaCl-unadapted tobacco cells became much higher in saline medium with 10 mm proline than in cells cultured without proline.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- 10 mm prolinee1
- Outcome
- proline contentse1
- Direction
- much highere1
- Comparator
- cultured without prolinee1
- Setting
- Na100-saline culture mediume1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco (Nicotiana tabacum L., cv. Bright Yellow-2)”
e1“The proline contents of the NaCl-unadapted cells became much higher when the cells were grown in Na100-saline culture medium with 10 mm proline than when the cells were cultured without proline”
Claim · model-written summary
Accumulation of K+, Na+ and counter ions was sufficient to compensate for the salinity-caused increase in water potential of the tobacco cells.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- accumulation of K+, Na+, counter ionse1
- Qualifier
- sufficient to compensate for the increase of the water potential of the cells caused by salinitye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco (Nicotiana tabacum L., cv. Bright Yellow-2)”
e1“The accumulation of K+, Na+, counter ions was sufficient to compensate for the increase of the water potential of the cells caused by salinity”
Claim · model-written summary
The authors suggest that exogenous proline does not act as a nitrogen store in tobacco cells.
- Taxon
- tobaccoNicotiana tabacume0
- Compound
- prolinee1
- Outcome
- nitrogen storee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco (Nicotiana tabacum L., cv. Bright Yellow-2)”
e1“exogenous proline does not act as a nitrogen store”
Claim · model-written summary
The authors suggest that proline may act as a protectant for enzymes and membranes against salt inactivation rather than as a compatible solute in tobacco suspension cells.
- Taxon
- tobaccoNicotiana tabacume0
- Compound
- prolinee0
- Outcome
- protectant for enzymes and membranes against salt inactivatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“proline may act as a protectant for enzymes and membranes against salt inactivation rather than as a compatible solute in tobacco suspension cells”
The same questions in other papers
proline · physiology: osmolytes · Nicotiana tabacum (tobacco)Results agree
2 papers in the same direction as this paper
- positive
- positive
proline · growth · Nicotiana tabacum (tobacco)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.