Abstract
Changes in transcript accumulation for cell wall-modifying proteins were examined in excised soybean root pieces colonized by soybean cyst nematodes (SCN), Heterodera glycines, using RT-PCR and soybean Affymetrix GeneChips. Sequence-specific PCR primer pairs were prepared from sequence data for core sequences in the GenBank soybean database and consensus sequences derived from the assembly of soybean ESTs. In addition, to identify previously uncharacterized soybean transcripts, degenerate primers were prepared for conserved motifs in cellulases (endo-1,4-beta-glucanases, EGases) and polygalacturonases (PGs) and these were used to amplify segments of transcripts that were then extended with 3' and 5' RACE. Several novel EGase and PG transcripts were identified. Gene expression patterns were determined by real-time RT-PCR for 11 EGases, three expansins (EXPs), 14 PGs, two pectate lyases (PLs), and two xyloglucan endotransglucosylase/hydrolases (XTHs) in soybean roots inoculated with SCN, non-inoculated roots, serial dissections of root tips, leaf abscission zones, flowers, apical buds, and expanding leaves. A large number of genes associated with cell wall modifications are strongly up-regulated in root pieces colonized by SCN. However, in contrast to most of the transcripts for cell wall proteins, two XTH transcripts were specifically down-regulated in the colonized root pieces. Gene expression in serial dissections of root tips (0-2 mm, and 2-7 mm) and whole roots indicate that the SCN up-regulated genes are associated with a wide range of developmental processes in roots. Also of interest, many of the cDNAs examined were up-regulated in petiole abscission zones induced to abscise with ethylene.
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Claims 4
Claim · model-written summary
A large number of cell wall modification genes were strongly up-regulated in soybean root pieces colonized by SCN.
- Taxon
- soybean
Glycine maxe0 - Organ / stage
- root piecese1
- Interactor
- soybean cyst nematodes
Heterodera glycinese0pest - Outcome
- genes associated with cell wall modificationse1
- Direction
- strongly up-regulatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“excised soybean root pieces colonized by soybean cyst nematodes (SCN), Heterodera glycines”
e1“A large number of genes associated with cell wall modifications are strongly up-regulated in root pieces colonized by SCN”
Claim · model-written summary
Two XTH transcripts were specifically down-regulated in the colonized soybean root pieces.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- colonized root piecese2
- Gene
- XTHe2
- Outcome
- XTH transcriptse2
- Direction
- down-regulatede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“excised soybean root pieces colonized by soybean cyst nematodes (SCN), Heterodera glycines”
e2“two XTH transcripts were specifically down-regulated in the colonized root pieces”
Claim · model-written summary
SCN up-regulated genes were associated with a wide range of developmental processes in soybean roots.
- Taxon
- soybeanGlycine maxe0
- Interactor
- SCNe3pest
- Outcome
- developmental processes in rootse3
- Direction
- up-regulatede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“excised soybean root pieces colonized by soybean cyst nematodes (SCN), Heterodera glycines”
e3“the SCN up-regulated genes are associated with a wide range of developmental processes in roots”
Claim · model-written summary
Many cDNAs were up-regulated in soybean petiole abscission zones induced to abscise with ethylene.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- petiole abscission zonese4
- Treatment
- ethylenee4
- Outcome
- cDNAse4
- Direction
- up-regulatede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“excised soybean root pieces colonized by soybean cyst nematodes (SCN), Heterodera glycines”
e4“many of the cDNAs examined were up-regulated in petiole abscission zones induced to abscise with ethylene”