Paper · 1979 · Journal of Experimental Botany

Peroxidase and Senescence

H. BIRECKA; M. J. CHASKES; J. GOLDSTEIN

11
claims
19
verbatim quotes
27
citations
2
research areas
—
Not open access

Abstract

Kinetin and a, á-dipyridyl prevented the rapid decrease of chlorophyll content in detached oat leaves senescing in the dark. In the light, detachment caused a 27–40% rise in peroxidase activity and kinetin enhanced the enzyme in the segments by about 80%. Darkness prevented any detachment-induced rise of the activity and decreased the stimulating action of kinetin and mechanical injury. The effect of dipyridyl on peroxidase activity in the dark was similar to that of kinetin. Kinetin enhanced the same distinctive isoperoxidases under light and dark conditions. Neither horseradish peroxidase nor that extracted from oat leaves showed any ability to hydroxylate free proline in vitro. A system which supposedly led to peroxidase-catalysed proline hydroxylation yielded small amounts of hydroxyproline in the absence of the enzyme. Staining with Fast Blue BB salt in the presence of IAA as a substrate after electrophoresis indicated that all detected oat isoperoxidases had an IAA oxidase activity visually paralleling their peroxidase activity. Crude extracts contained IAA oxidase inhibitors that could be partially or fully removed by dialysis. The possible significance of the rise in peroxidase activity during senescence is discussed.

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Claims 11

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Kinetin prevented chlorophyll loss in detached oat leaves senescing in darkness.

Treatment
Kinetine0
Outcome
chlorophyll contente0
Direction
preventede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Avena sativa
Avena sativa · TaxonSendelbach ( talk ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Kinetin and a, á-dipyridyl prevented the rapid decrease of chlorophyll content in detached oat leaves senescing in the dark.”

✓ located verbatim in the source · abstract, characters 0–124 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Dipyridyl prevented chlorophyll loss in detached oat leaves senescing in darkness.

Treatment
a, á-dipyridyle0
Outcome
chlorophyll contente0
Direction
preventede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Avena sativa
Avena sativa · TaxonSendelbach ( talk ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Kinetin and a, á-dipyridyl prevented the rapid decrease of chlorophyll content in detached oat leaves senescing in the dark.”

✓ located verbatim in the source · abstract, characters 0–124 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

In light, detachment raised peroxidase activity by 27–40%.

Treatment
detachmente0
Outcome
peroxidase activitye0
Direction
risee0
Value
27–40%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Avena sativa
Avena sativa · TaxonSendelbach ( talk ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“In the light, detachment caused a 27–40% rise in peroxidase activity”

✓ located verbatim in the source · abstract, characters 125–193 · show in text ↑
e7

“detached oat leaves senescing in the dark.”

✓ located verbatim in the source · abstract, characters 82–124 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

In light, kinetin enhanced peroxidase activity by about 80%.

Treatment
kinetine0
Outcome
peroxidase activitye1
Direction
enhancede0
Value
about 80%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Avena sativa
Avena sativa · TaxonSendelbach ( talk ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“kinetin enhanced the enzyme in the segments by about 80%.”

✓ located verbatim in the source · abstract, characters 198–255 · show in text ↑
e1

“peroxidase activity and kinetin enhanced the enzyme in the segments by about 80%.”

✓ located verbatim in the source · abstract, characters 174–255 · show in text ↑
e7

“detached oat leaves senescing in the dark.”

✓ located verbatim in the source · abstract, characters 82–124 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Darkness prevented the detachment-related rise in peroxidase and reduced stimulation by kinetin and injury.

Outcome
activitye0
Setting
Darknesse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Avena sativa
Avena sativa · TaxonSendelbach ( talk ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Darkness prevented any detachment-induced rise of the activity and decreased the stimulating action of kinetin and mechanical injury.”

✓ located verbatim in the source · abstract, characters 256–389 · show in text ↑
e7

“detached oat leaves senescing in the dark.”

✓ located verbatim in the source · abstract, characters 82–124 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Dipyridyl affected dark peroxidase activity similarly to kinetin.

Treatment
dipyridyle0
Outcome
peroxidase activitye0
Direction
similare0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Avena sativa
Avena sativa · TaxonSendelbach ( talk ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The effect of dipyridyl on peroxidase activity in the dark was similar to that of kinetin.”

✓ located verbatim in the source · abstract, characters 390–480 · show in text ↑
e7

“detached oat leaves senescing in the dark.”

✓ located verbatim in the source · abstract, characters 82–124 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Kinetin enhanced the same distinctive isoperoxidases in light and dark.

Treatment
Kinetine0
Outcome
isoperoxidasese0
Direction
enhancede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Avena sativa
Avena sativa · TaxonSendelbach ( talk ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Kinetin enhanced the same distinctive isoperoxidases under light and dark conditions.”

✓ located verbatim in the source · abstract, characters 481–566 · show in text ↑
e7

“detached oat leaves senescing in the dark.”

✓ located verbatim in the source · abstract, characters 82–124 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

Neither horseradish nor oat peroxidase hydroxylated free proline in vitro.

Outcome
hydroxylate free prolinee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Avena sativa
Avena sativa · TaxonSendelbach ( talk ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Neither horseradish peroxidase nor that extracted from oat leaves showed any ability to hydroxylate free proline in vitro.”

✓ located verbatim in the source · abstract, characters 567–689 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

The putative peroxidase-catalyzed system produced some hydroxyproline without enzyme.

Outcome
hydroxyprolinee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Avena sativa
Avena sativa · TaxonSendelbach ( talk ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“A system which supposedly led to peroxidase-catalysed proline hydroxylation yielded small amounts of hydroxyproline in the absence of the enzyme.”

✓ located verbatim in the source · abstract, characters 690–835 · show in text ↑
e7

“detached oat leaves senescing in the dark.”

✓ located verbatim in the source · abstract, characters 82–124 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

All detected oat isoperoxidases had IAA oxidase activity paralleling peroxidase activity.

Outcome
IAA oxidase activitye0
Direction
parallelinge0
Comparator
peroxidase activitye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Avena sativa
Avena sativa · TaxonSendelbach ( talk ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“all detected oat isoperoxidases had an IAA oxidase activity visually paralleling their peroxidase activity.”

✓ located verbatim in the source · abstract, characters 943–1050 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Crude extracts contained IAA oxidase inhibitors removable by dialysis.

Treatment
dialysise0
Outcome
IAA oxidase inhibitorse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Avena sativa
Avena sativa · TaxonSendelbach ( talk ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Crude extracts contained IAA oxidase inhibitors that could be partially or fully removed by dialysis.”

✓ located verbatim in the source · abstract, characters 1051–1152 · show in text ↑
e7

“detached oat leaves senescing in the dark.”

✓ located verbatim in the source · abstract, characters 82–124 · show in text ↑

kinetin · physiology: pigments · Avena sativa (oats)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.