Abstract
We previously showed that during protoplast isolation, an oxidative burst occurred and the generation of active oxygen species was differentially mediated in tobacco (Nicotiana tabacum) and grapevine (Vitis vinifera), accompanied by significant quantitative differences (A.K. Papadakis, K.A. Roubelakis-Angelakis [1999] Plant Physiol 127: 197-205). We have now further tested if the expression of totipotency in protoplasts is related to the activity of cellular antioxidant machinery during protoplast culture. Totipotent (T) tobacco protoplasts had 2-fold lower contents of intracellular O2*- and H2O2 and 7-fold lower levels of O2*- and H2O2 in the culture medium, compared with non-totipotent (NT) tobacco protoplasts. Addition of alkaline dimethylsulfoxide, known to generate O2*-, resulted in isolation of tobacco protoplasts with reduced viability and cell division potential during subsequent culture. Active oxygen species levels decreased in tobacco and grapevine protoplasts during culturing, although higher contents of O2*- and H2O2 were still found in NT- compared with T-tobacco protoplasts, after 8 d in culture. In T-tobacco protoplasts, the reduced forms of ascorbate and glutathione predominated, whereas in NT-tobacco and grapevine protoplasts, the oxidized forms predominated. In addition, T-tobacco protoplasts exhibited severalfold lower lipid peroxidation than NT-tobacco and grapevine protoplasts. Furthermore, several antioxidant enzyme activities were increased in T-tobacco protoplasts. Superoxide dismutase activity increased in tobacco, but not in grapevine protoplasts during culturing due to the increased expression of cytoplasmic Cu/Zn-superoxide dismutase. The increase was only sustained in T-tobacco protoplasts for d 8. Together, these results suggest that suppressed expression of totipotency in protoplasts is correlated with reduced activity of the cellular antioxidant machinery.
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Claims 15
Claim · model-written summary
Totipotent tobacco protoplasts had twofold lower intracellular reactive oxygen species than non-totipotent ones.
- Taxon
- tobacco
Nicotiana tabacume0 - Material
- Totipotent (T) tobacco protoplastse0
- Outcome
- intracellular O2*- and H2O2e0
- Direction
- lowere0
- Value
- 2-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Totipotent (T) tobacco protoplasts had 2-fold lower contents of intracellular O2*- and H2O2”
Claim · model-written summary
Totipotent tobacco protoplasts had sevenfold lower culture-medium reactive oxygen species.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- levels of O2*- and H2O2 in the culture mediume0
- Direction
- lowere0
- Value
- 7-folde0
- Comparator
- non-totipotent (NT) tobacco protoplastse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“7-fold lower levels of O2*- and H2O2 in the culture medium, compared with non-totipotent (NT) tobacco protoplasts”
Claim · model-written summary
Alkaline dimethylsulfoxide reduced tobacco protoplast viability and cell division potential.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Addition of alkaline dimethylsulfoxidee0
- Outcome
- viability and cell division potentiale0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of alkaline dimethylsulfoxide, known to generate O2*-, resulted in isolation of tobacco protoplasts with reduced viability and cell division potential during subsequent culture”
Claim · model-written summary
Active oxygen species decreased in tobacco protoplast culture.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- Active oxygen species levelse0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Active oxygen species levels decreased in tobacco and grapevine protoplasts during culturing”
Claim · model-written summary
Active oxygen species decreased in grapevine protoplast culture.
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Active oxygen species levels decreased in tobacco and grapevine protoplasts during culturing”
Claim · model-written summary
Non-totipotent tobacco protoplasts retained higher reactive oxygen species after eight days.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- NT-e0
- Outcome
- contents of O2*- and H2O2e0
- Direction
- highere0
- Qualifier
- after 8 d in culturee0
- Comparator
- T-tobacco protoplastse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher contents of O2*- and H2O2 were still found in NT- compared with T-tobacco protoplasts, after 8 d in culture”
Claim · model-written summary
Reduced ascorbate and glutathione predominated in totipotent tobacco protoplasts.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- T-tobacco protoplastse0
- Outcome
- reduced forms of ascorbate and glutathionee0
- Direction
- predominatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In T-tobacco protoplasts, the reduced forms of ascorbate and glutathione predominated”
Claim · model-written summary
Oxidized ascorbate and glutathione predominated in non-totipotent tobacco protoplasts.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- NT-tobaccoe0
- Outcome
- oxidized formse0
- Direction
- predominatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“whereas in NT-tobacco and grapevine protoplasts, the oxidized forms predominated”
Claim · model-written summary
Oxidized ascorbate and glutathione predominated in grapevine protoplasts.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“whereas in NT-tobacco and grapevine protoplasts, the oxidized forms predominated”
Claim · model-written summary
Totipotent tobacco protoplasts had lower lipid peroxidation than non-totipotent tobacco and grapevine protoplasts.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- T-tobacco protoplastse0
- Outcome
- lipid peroxidatione0
- Direction
- lowere0
- Value
- severalfolde0
- Comparator
- NT-tobacco and grapevine protoplastse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“T-tobacco protoplasts exhibited severalfold lower lipid peroxidation than NT-tobacco and grapevine protoplasts”
Claim · model-written summary
Several antioxidant enzyme activities increased in totipotent tobacco protoplasts.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- T-tobacco protoplastse0
- Outcome
- antioxidant enzyme activitiese0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“several antioxidant enzyme activities were increased in T-tobacco protoplasts”
Claim · model-written summary
Superoxide dismutase activity increased in cultured tobacco protoplasts.
- Taxon
- tobaccoNicotiana tabacume0
- Gene
- Superoxide dismutasee0
- Outcome
- Superoxide dismutase activitye0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Superoxide dismutase activity increased in tobacco, but not in grapevine protoplasts during culturing”
Claim · model-written summary
Superoxide dismutase activity did not increase in grapevine protoplasts during culture.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Superoxide dismutase activity increased in tobacco, but not in grapevine protoplasts during culturing”
Claim · model-written summary
Increased cytoplasmic Cu/Zn-superoxide dismutase expression accounted for tobacco enzyme activity.
- Gene
- cytoplasmic Cu/Zn-superoxide dismutasee0
- Outcome
- increased expression of cytoplasmic Cu/Zn-superoxide dismutasee0
Not reportedTaxon
Verbatim
e0“due to the increased expression of cytoplasmic Cu/Zn-superoxide dismutase”
Claim · model-written summary
The superoxide dismutase activity increase persisted to day eight only in totipotent tobacco protoplasts.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- T-tobacco protoplastse0
- Outcome
- The increasee0
- Direction
- sustainede0
- Qualifier
- for d 8e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The increase was only sustained in T-tobacco protoplasts for d 8”
The same questions in other papers
dimethyl sulfoxide · cytology · Nicotiana tabacum (tobacco)Results agree
1 paper in the same direction as this paper
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.