Abstract
SGT1 (Suppressor of G2 allele of SKP1) is required to maintain plant disease Resistance (R) proteins with Nucleotide-Binding (NB) and Leucine-Rich Repeat (LRR) domains in an inactive but signaling-competent state. SGT1 is an integral component of a multi-protein network that includes RACK1, Rac1, RAR1, Rboh, HSP90 and HSP70, and in rice the Mitogen-Activated Protein Kinase (MAPK), OsMAPK6. Tobacco (Nicotiana tabacum) N protein, which belongs to the Toll-Interleukin Receptor (TIR)-NB-LRR class of R proteins, confers resistance to Tobacco Mosaic Virus (TMV). Following transient expression in planta, we analyzed the functional relationship between SGT1, SIPK - a tobacco MAPK6 ortholog - and N, using mass spectrometry, confocal microscopy and pathogen assays. Here, we show that tobacco SGT1 undergoes specific phosphorylation in a canonical MAPK target-motif by SIPK. Mutation of this motif to mimic SIPK phosphorylation leads to an increased proportion of cells displaying SGT1 nuclear accumulation and impairs N-mediated resistance to TMV, as does phospho-null substitution at the same residue. Forced nuclear localization of SGT1 causes N to be confined to nuclei. Our data suggest that one mode of regulating nucleocytoplasmic partitioning of R proteins is by maintaining appropriate levels of SGT1 phosphorylation catalyzed by plant MAPK.
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Claims 6
Claim · model-written summary
Tobacco SGT1 undergoes specific phosphorylation at a canonical MAPK target motif by SIPK.
- Taxon
- tobacco
Nicotiana tabacumt0 - Gene
- SGT1e1
- Outcome
- specific phosphorylatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco N receptor”
e1“tobacco SGT1 undergoes specific phosphorylation in a canonical MAPK target-motif by SIPK”
Claim · model-written summary
Mimicking SIPK phosphorylation of the SGT1 motif increases the proportion of cells with SGT1 nuclear accumulation.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Mutation of this motif to mimic SIPK phosphorylatione2
- Gene
- SGT1e2
- Outcome
- proportion of cells displaying SGT1 nuclear accumulatione2
- Direction
- increasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco N receptor”
e2“Mutation of this motif to mimic SIPK phosphorylation leads to an increased proportion of cells displaying SGT1 nuclear accumulation”
Claim · model-written summary
Mimicking SIPK phosphorylation of SGT1 impairs N-mediated resistance to TMV.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Mutation of this motif to mimic SIPK phosphorylatione3
- Interactor
- TMV
Tobacco mosaic viruse3pathogen - Outcome
- N-mediated resistance to TMVe3
- Direction
- impairse3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco N receptor”
e3“Mutation of this motif to mimic SIPK phosphorylation leads to an increased proportion of cells displaying SGT1 nuclear accumulation and impairs N-mediated resistance to TMV”
Claim · model-written summary
Phospho-null substitution at the same SGT1 residue also impairs N-mediated resistance to TMV.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- phospho-null substitution at the same residuee4
- Interactor
- TMVTobacco mosaic viruse4pathogen
- Outcome
- N-mediated resistance to TMVe4
- Direction
- impairse4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco N receptor”
e4“impairs N-mediated resistance to TMV, as does phospho-null substitution at the same residue”
Claim · model-written summary
Forced nuclear localization of SGT1 confines N to nuclei.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Forced nuclear localization of SGT1e5
- Gene
- SGT1e5
- Outcome
- N to be confined to nucleie5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco N receptor”
e5“Forced nuclear localization of SGT1 causes N to be confined to nuclei”
Claim · model-written summary
Regulating SGT1 phosphorylation levels by plant MAPK may be one mode of regulating nucleocytoplasmic partitioning of R proteins.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- SGT1e6
- Outcome
- nucleocytoplasmic partitioning of R proteinse6
- Qualifier
- maintaining appropriate levels of SGT1 phosphorylatione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco N receptor”
e6“Our data suggest that one mode of regulating nucleocytoplasmic partitioning of R proteins is by maintaining appropriate levels of SGT1 phosphorylation catalyzed by plant MAPK”
The same questions in other papers
loss of function · Tobacco mosaic virus · Nicotiana tabacum (tobacco)Positive and negative results
11 papers in the same direction as this paper · 6 in the opposite direction
- negative
- negative
- positive
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See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.