Abstract
A full-length drought-responsive gene Ccrboh, encoding the respiratory burst oxidase homologue (rboh), was cloned in Citrullus colocynthis, a very drought-tolerant cucurbit species. The robh protein, also named NADPH oxidase, is conserved in plants and animals, and functions in the production of reactive oxygen species (ROS). The Ccrboh gene accumulated in a tissue-specific pattern when C. colocynthis was treated with PEG, abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), or NaCl, while the homologous rboh gene did not show any change in C. lanatus var. lanatus, cultivated watermelon, during drought. Grafting experiments were conducted using C. colocynthis or C. lanatus as the rootstock or scion. Results showed that the rootstock significantly affects gene expression in the scion, and some signals might be transported from the root to the shoot. Ccrboh in C. colocynthis was found to function early during plant development, reaching high mRNA transcript levels 3 d after germination. The subcellular location of Ccrboh was investigated by transient expression of the 35S::Ccrboh::GFP fusion construct in protoplasts. The result confirmed that Ccrboh is a transmembrane protein. Our data suggest that Ccrboh might be functionally important during the acclimation of plants to stress and also in plant development. It holds great promise for improving drought tolerance of other cucurbit species.
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Claims 7
Claim · model-written summary
A full-length drought-responsive Ccrboh gene was cloned in Citrullus colocynthis.
- Taxon
- Citrullus colocynthis
Citrullus colocynthise0 - Gene
- Ccrbohe0
- Outcome
- full-length drought-responsive gene Ccrbohe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A full-length drought-responsive gene Ccrboh, encoding the respiratory burst oxidase homologue (rboh), was cloned in Citrullus colocynthis”
Claim · model-written summary
Ccrboh accumulation showed tissue-specific patterns under PEG, ABA, SA, JA and NaCl treatments.
- Taxon
- C. colocynthisCitrullus colocynthise0
- Treatment
- PEG, abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), or NaCle0
- Gene
- Ccrbohe0
- Outcome
- Ccrboh genee0
- Direction
- accumulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Ccrboh gene accumulated in a tissue-specific pattern when C. colocynthis was treated with PEG, abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), or NaCl”
Claim · model-written summary
The homologous rboh gene showed no change in cultivated watermelon during drought.
- Taxon
- C. lanatus var. lanatus
Citrullus lanatuse0 - Treatment
- droughte0
- Gene
- rbohe0
- Outcome
- rboh genee0
- Direction
- did not show any changee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the homologous rboh gene did not show any change in C. lanatus var. lanatus, cultivated watermelon, during drought”
Claim · model-written summary
Rootstock significantly affected gene expression in the scion.
- Taxon
- Citrullus colocynthisCitrullus colocynthist0
- Treatment
- rootstocke0
- Outcome
- gene expression in the scione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the rootstock significantly affects gene expression in the scion”
t0“Citrullus colocynthis”
Claim · model-written summary
Ccrboh transcript levels were high three days after germination.
- Taxon
- C. colocynthisCitrullus colocynthise0
- Gene
- Ccrbohe0
- Outcome
- mRNA transcript levelse0
- Direction
- highe0
- Qualifier
- 3 d after germinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ccrboh in C. colocynthis was found to function early during plant development, reaching high mRNA transcript levels 3 d after germination”
Claim · model-written summary
Ccrboh was confirmed to be a transmembrane protein.
- Taxon
- Citrullus colocynthisCitrullus colocynthist0
- Gene
- Ccrbohe0
- Outcome
- transmembrane proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The result confirmed that Ccrboh is a transmembrane protein”
t0“Citrullus colocynthis”
Claim · model-written summary
The authors suggest Ccrboh may matter for stress acclimation and plant development.
- Taxon
- Citrullus colocynthisCitrullus colocynthist0
- Gene
- Ccrbohe0
- Outcome
- acclimation of plants to stress and also in plant developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ccrboh might be functionally important during the acclimation of plants to stress and also in plant development”
t0“Citrullus colocynthis”
The same questions in other papers
drought · molecular: expression · Citrullus lanatus (watermelon)Positive and negative results
2 in the opposite direction
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.