Abstract
Abstract It is proposed that desiccation tolerance in the embryo of seeds depends upon the capacity to repair damage to genomic DNA when the desiccated embryo is rehydrated. From a study of imbibed and hydrated embryos of rye ( Secale cereale ) and wild oat ( Avena fatua ) evidence is provided that it is neither the extent of water uptake by the cells, the ensuing stability of the DNA to desiccation, nor the onset of S-phase DNA synthesis in the first cell cycle of germination that determines whether the desiccated embryo will survive. It is shown that when α- and β-polymerases of DNA repair are inhibited by aphidicolin and dideoxythymidine-5'-triphosphate, respectively, a γ-irradiation-induced DNA fragmentation cannot be fully repaired. It is shown that in hydrated embryos, at a stage when desiccation tolerance is lost, embryo cells still repair irradiation-induced damage, but this repaired DNA is unstable to desiccation and cannot be rerepaired when water is again made available. The failure to re-repair on rehydration appears to be critical to embryo survival and successful germination.
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Claims 6
Claim · model-written summary
The authors propose that desiccation tolerance of the seed embryo depends on its capacity to repair damage to genomic DNA when rehydrated.
- Taxon
- rye ( Secale cereale ) and wild oat ( Avena fatua )
Avena fatua
Secale cerealee1 - Organ / stage
- embryo of seedse0
- Outcome
- desiccation tolerancee0
- Qualifier
- capacity to repair damage to genomic DNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It is proposed that desiccation tolerance in the embryo of seeds depends upon the capacity to repair damage to genomic DNA”
e1“rye ( Secale cereale ) and wild oat ( Avena fatua )”
Claim · model-written summary
In imbibed and hydrated rye and wild oat embryos, neither the extent of water uptake, the stability of the DNA to desiccation, nor the onset of S-phase DNA synthesis determines whether the desiccated embryo survives.
- Taxon
- rye ( Secale cereale ) and wild oat ( Avena fatua )Avena fatuaSecale cerealee1
- Outcome
- whether the desiccated embryo will survivee0
- Qualifier
- extent of water uptake by the cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it is neither the extent of water uptake by the cells, the ensuing stability of the DNA to desiccation, nor the onset of S-phase DNA synthesis in the first cell cycle of germination that determines whether the desiccated embryo will survive”
e1“rye ( Secale cereale ) and wild oat ( Avena fatua )”
Claim · model-written summary
When DNA repair polymerases alpha and beta are inhibited by aphidicolin and dideoxythymidine triphosphate, gamma-irradiation-induced DNA fragmentation cannot be fully repaired.
- Taxon
- rye ( Secale cereale ) and wild oat ( Avena fatua )Avena fatuaSecale cerealee1
- Treatment
- aphidicolin and dideoxythymidine-5'-triphosphatee0
- Outcome
- DNA fragmentatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when α- and β-polymerases of DNA repair are inhibited by aphidicolin and dideoxythymidine-5'-triphosphate, respectively, a γ-irradiation-induced DNA fragmentation cannot be fully repaired”
e1“rye ( Secale cereale ) and wild oat ( Avena fatua )”
Claim · model-written summary
In hydrated embryos at a stage when desiccation tolerance is lost, embryo cells still repair irradiation-induced DNA damage.
- Taxon
- rye ( Secale cereale ) and wild oat ( Avena fatua )Avena fatuaSecale cerealee1
- Outcome
- embryo cells still repair irradiation-induced damagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“embryo cells still repair irradiation-induced damage”
e1“rye ( Secale cereale ) and wild oat ( Avena fatua )”
Claim · model-written summary
In hydrated embryos at the stage when desiccation tolerance is lost, the repaired DNA is unstable to desiccation and cannot be rerepaired when water is again made available.
- Taxon
- rye ( Secale cereale ) and wild oat ( Avena fatua )Avena fatuaSecale cerealee1
- Outcome
- this repaired DNA is unstable to desiccation and cannot be rerepairede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this repaired DNA is unstable to desiccation and cannot be rerepaired when water is again made available”
e1“rye ( Secale cereale ) and wild oat ( Avena fatua )”
Claim · model-written summary
The failure to re-repair DNA on rehydration appears to be critical to embryo survival and successful germination.
- Taxon
- rye ( Secale cereale ) and wild oat ( Avena fatua )Avena fatuaSecale cerealee1
- Outcome
- embryo survival and successful germinatione0
- Qualifier
- failure to re-repair on rehydratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The failure to re-repair on rehydration appears to be critical to embryo survival and successful germination”
e1“rye ( Secale cereale ) and wild oat ( Avena fatua )”