Abstract
Gibberellin (GA) biosynthesis is regulated by feedback control providing a mechanism for GA homeostasis in plants. However, regulatory elements involved in the feedback control are not known. In this report, we show that a rice (Oryza sativa) YABBY1 (YAB1) gene had a similar expression pattern as key rice GA biosynthetic genes GA3ox2 and GA20ox2. Overexpression of YAB1 in transgenic rice resulted in a semidwarf phenotype that could be fully rescued by applied GA. Quantification of the endogenous GA content revealed increases of GA(20) and decreases of GA(1) levels in the overexpression plants, in which the transcripts of the biosynthetic gene GA3ox2 were decreased. Cosuppression of YAB1 in transgenic plants induced expression of GA3ox2. The repression of GA3ox2 could be obtained upon treatment by dexamethasone of transgenic plants expressing a YAB1-glucocorticoid receptor fusion. Importantly, we show that YAB1 bound to a GA-responsive element within the GA3ox2 promoter. In addition, the expression of YAB1 was deregulated in GA biosynthesis and signaling mutants and could be either transiently induced by GA or repressed by a GA inhibitor. Finally, either overexpression or cosuppression of YAB1 impaired GA-mediated repression of GA3ox2. These data together suggest that YAB1 is involved in the feedback regulation of GA biosynthesis in rice.
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Claims 14
Claim · model-written summary
YAB1 expression resembled expression of GA3ox2 and GA20ox2.
- Taxon
- rice
Oryza sativae0 - Gene
- YABBY1 (YAB1)e0
- Outcome
- similar expression patterne0
- Comparator
- GA3ox2 and GA20ox2e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a rice (Oryza sativa) YABBY1 (YAB1) gene had a similar expression pattern as key rice GA biosynthetic genes GA3ox2 and GA20ox2”
Claim · model-written summary
YAB1 overexpression caused a semidwarf phenotype.
- Taxon
- riceOryza sativae0
- Treatment
- Overexpression of YAB1e0
- Gene
- YAB1e0
- Outcome
- semidwarf phenotypee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of YAB1 in transgenic rice resulted in a semidwarf phenotype”
Claim · model-written summary
Applied GA fully rescued the YAB1 overexpression semidwarf phenotype.
- Taxon
- riceOryza sativae0
- Treatment
- applied GAe0
- Gene
- YAB1e0
- Outcome
- semidwarf phenotypee0
- Direction
- fully rescuede0
- Comparator
- Overexpression of YAB1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of YAB1 in transgenic rice resulted in a semidwarf phenotype that could be fully rescued by applied GA”
Claim · model-written summary
YAB1 overexpression increased GA(20).
- Taxon
- RiceOryza sativat0
- Treatment
- overexpressione0
- Outcome
- GA(20)e0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increases of GA(20) and decreases of GA(1) levels in the overexpression plants”
t0“Rice”
Claim · model-written summary
YAB1 overexpression decreased GA(1).
- Taxon
- RiceOryza sativat0
- Treatment
- overexpressione0
- Outcome
- GA(1) levelse0
- Direction
- decreasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increases of GA(20) and decreases of GA(1) levels in the overexpression plants”
t0“Rice”
Claim · model-written summary
GA3ox2 transcripts decreased in YAB1 overexpression plants.
- Taxon
- RiceOryza sativat0
- Treatment
- overexpression plantse1
- Gene
- GA3ox2e0
- Outcome
- transcripts of the biosynthetic gene GA3ox2e0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcripts of the biosynthetic gene GA3ox2 were decreased”
e1“overexpression plants”
t0“Rice”
Claim · model-written summary
YAB1 cosuppression induced GA3ox2 expression.
- Taxon
- RiceOryza sativat0
- Treatment
- Cosuppression of YAB1e0
- Gene
- GA3ox2e0
- Outcome
- expression of GA3ox2e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cosuppression of YAB1 in transgenic plants induced expression of GA3ox2”
t0“Rice”
Claim · model-written summary
Dexamethasone treatment repressed GA3ox2 in YAB1-glucocorticoid receptor plants.
- Taxon
- RiceOryza sativat0
- Treatment
- treatment by dexamethasonee0
- Gene
- GA3ox2e0
- Outcome
- GA3ox2e0
- Direction
- repressione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The repression of GA3ox2 could be obtained upon treatment by dexamethasone of transgenic plants expressing a YAB1-glucocorticoid receptor fusion”
t0“Rice”
Claim · model-written summary
YAB1 bound a GA-responsive element in the GA3ox2 promoter.
- Taxon
- RiceOryza sativat0
- Gene
- YAB1e0
- Outcome
- bound to a GA-responsive element within the GA3ox2 promotere0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“YAB1 bound to a GA-responsive element within the GA3ox2 promoter”
t0“Rice”
Claim · model-written summary
YAB1 expression was deregulated in GA biosynthesis and signaling mutants.
- Taxon
- RiceOryza sativat0
- Material
- GA biosynthesis and signaling mutantse0
- Gene
- YAB1e0
- Outcome
- expression of YAB1 was deregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of YAB1 was deregulated in GA biosynthesis and signaling mutants”
t0“Rice”
Claim · model-written summary
GA transiently induced YAB1 expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transiently induced by GA”
e1“the expression of YAB1”
t0“Rice”
Claim · model-written summary
A GA inhibitor repressed YAB1 expression.
- Taxon
- RiceOryza sativat0
- Treatment
- GA inhibitore0
- Gene
- YAB1e1
- Outcome
- expression of YAB1e1
- Direction
- repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“repressed by a GA inhibitor”
e1“the expression of YAB1”
t0“Rice”
Claim · model-written summary
YAB1 overexpression impaired GA-mediated GA3ox2 repression.
- Taxon
- RiceOryza sativat0
- Treatment
- overexpressione0
- Gene
- GA3ox2e0
- Outcome
- GA-mediated repression of GA3ox2e0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“either overexpression or cosuppression of YAB1 impaired GA-mediated repression of GA3ox2”
t0“Rice”
Claim · model-written summary
YAB1 cosuppression impaired GA-mediated GA3ox2 repression.
- Taxon
- RiceOryza sativat0
- Treatment
- cosuppression of YAB1e0
- Gene
- GA3ox2e0
- Outcome
- GA-mediated repression of GA3ox2e0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“either overexpression or cosuppression of YAB1 impaired GA-mediated repression of GA3ox2”
t0“Rice”
The same questions in other papers
Gibberellic acid · molecular: expression · Oryza sativa (rice)Positive and negative results
17 papers in the same direction as this paper · 7 in the opposite direction
- no effect
- no effect
- no effect
21 papers more in this group
See all 25 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.