Abstract
Oxygen uptake rates are increased when concentrated ammonium instead of nitrate is used as sole N source. Several explanations for this increased respiration have been suggested, but the underlying mechanisms are still unclear. To investigate possible factors responsible for this respiratory increase, we measured the O₂ uptake rate, activity and transcript level of respiratory components, and concentration of adenylates using Arabidopsis thaliana shoots grown in media containing various N sources. The O₂ uptake rate was correlated with concentrations of ammonium and ATP in shoots, but not related to the ammonium assimilation. The capacity of the ATP-coupling cytochrome pathway (CP) and its related genes were up-regulated when concentrated ammonium was sole N source, whereas the ATP-uncoupling alternative oxidase did not influence the extent of the respiratory increase. Our results suggest that the ammonium-dependent increase of the O₂ uptake rate can be explained by the up-regulation of the CP, which may be related to the ATP consumption by the plasma-membrane H+ -ATPase.
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Claims 8
Claim · model-written summary
Shoot oxygen uptake correlated with ammonium concentration.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Organ / stage
- shootse0
- Compound
- ammoniume0
- Outcome
- O₂ uptake ratee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The O₂ uptake rate was correlated with concentrations of ammonium and ATP in shoots”
e1“Arabidopsis thaliana shoots”
Claim · model-written summary
Shoot oxygen uptake correlated with ATP concentration.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Organ / stage
- shootse0
- Compound
- ATPe0
- Outcome
- O₂ uptake ratee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The O₂ uptake rate was correlated with concentrations of ammonium and ATP in shoots”
e1“Arabidopsis thaliana shoots”
Claim · model-written summary
Shoot oxygen uptake was unrelated to ammonium assimilation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Organ / stage
- shootse0
- Outcome
- O₂ uptake ratee0
- Direction
- not relatede0
- Qualifier
- ammonium assimilatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The O₂ uptake rate was correlated with concentrations of ammonium and ATP in shoots, but not related to the ammonium assimilation.”
e1“Arabidopsis thaliana shoots”
Claim · model-written summary
Concentrated ammonium as sole nitrogen source increased ATP-coupling cytochrome pathway capacity.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- concentrated ammoniume0
- Outcome
- capacity of the ATP-coupling cytochrome pathwaye0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The capacity of the ATP-coupling cytochrome pathway (CP) and its related genes were up-regulated when concentrated ammonium was sole N source”
e1“Arabidopsis thaliana shoots”
Claim · model-written summary
Concentrated ammonium as sole nitrogen source up-regulated genes related to the cytochrome pathway.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- concentrated ammoniume0
- Outcome
- related genese0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The capacity of the ATP-coupling cytochrome pathway (CP) and its related genes were up-regulated when concentrated ammonium was sole N source”
e1“Arabidopsis thaliana shoots”
Claim · model-written summary
Alternative oxidase did not influence the extent of the respiratory increase.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- alternative oxidasee0
- Outcome
- extent of the respiratory increasee0
- Direction
- did not influencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ATP-uncoupling alternative oxidase did not influence the extent of the respiratory increase”
e1“Arabidopsis thaliana shoots”
Claim · model-written summary
The authors propose cytochrome pathway up-regulation explains ammonium-dependent oxygen uptake increase.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Outcome
- O₂ uptake ratee0
- Qualifier
- up-regulation of the CPe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ammonium-dependent increase of the O₂ uptake rate can be explained by the up-regulation of the CP”
e1“Arabidopsis thaliana shoots”
Claim · model-written summary
The authors suggest cytochrome pathway up-regulation may relate to ATP consumption by plasma-membrane H+ -ATPase.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- plasma-membrane H+ -ATPasee0
- Outcome
- ATP consumptione0
- Qualifier
- may be relatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which may be related to the ATP consumption by the plasma-membrane H+ -ATPase”
e1“Arabidopsis thaliana shoots”
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See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.