Abstract
We have previously determined that phospholipase D (PLD) is activated by abscisic acid (ABA), and this activation is required for the ABA response of the cereal aleurone cell. In this study, ABA-stimulated PLD activity was reconstituted in vitro in microsomal membranes prepared from aleurone protoplasts. The transient nature (20 min) and degree (1.5- to 2-fold) of activation in vitro were similar to that measured in vivo. Stimulation by ABA was only apparent in the membrane fraction and was associated with a fraction enriched in plasma membrane. These results suggest that an ABA receptor system and elements linking it to PLD activation are associated with the aleurone plasma membrane. The activation of PLD in vitro by ABA was dependent on the presence of GTP. Addition of GTPgammaS transiently stimulated PLD in an ABA-independent manner, whereas treatment with GDPbetaS or pertussis toxin blocked the PLD activation by ABA. Application of pertussis toxin to intact aleurone protoplasts inhibited the ability of ABA to activate PLD as well as antagonizing the ability of ABA to down-regulate gibberellic acid-stimulated alpha-amylase production. All of these data support the hypothesis that ABA stimulation of PLD activity occurs at the plasma membrane and is mediated by G-protein activity.
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Claims 10
Claim · model-written summary
ABA activation of PLD lasted 20 min and was 1.5 to 2 fold in vitro, similar to in vivo.
- Taxon
- Barley
.jpg?width=60)
Hordeum vulgaret0 - Outcome
- activation in vitroe0
- Value
- 1.5- to 2-folde0
- Qualifier
- 20 mine0
- Comparator
- in vivoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The transient nature (20 min) and degree (1.5- to 2-fold) of activation in vitro were similar to that measured in vivo.”
t0“Barley”
Claim · model-written summary
ABA stimulated PLD only in a membrane fraction enriched in plasma membrane.
- Taxon
- BarleyHordeum vulgaret0
- Organ / stage
- plasma membranee0
- Treatment
- ABAe0
- Outcome
- Stimulatione0
- Direction
- Stimulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Stimulation by ABA was only apparent in the membrane fraction and was associated with a fraction enriched in plasma membrane.”
t0“Barley”
Claim · model-written summary
ABA activation of PLD depended on GTP.
- Taxon
- BarleyHordeum vulgaret0
- Gene
- PLDe0
- Compound
- GTPe0
- Outcome
- activation of PLDe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activation of PLD in vitro by ABA was dependent on the presence of GTP.”
t0“Barley”
Claim · model-written summary
GTPgammaS transiently stimulated PLD without ABA.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- GTPgammaSe0
- Outcome
- PLDe0
- Direction
- stimulatede0
- Comparator
- ABA-independente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of GTPgammaS transiently stimulated PLD in an ABA-independent manner”
t0“Barley”
Claim · model-written summary
GDPbetaS blocked ABA activation of PLD.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- GDPbetaSe0
- Outcome
- PLD activatione0
- Direction
- blockede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment with GDPbetaS or pertussis toxin blocked the PLD activation by ABA”
t0“Barley”
Claim · model-written summary
Pertussis toxin blocked ABA activation of PLD.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- pertussis toxine0
- Outcome
- PLD activatione0
- Direction
- blockede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment with GDPbetaS or pertussis toxin blocked the PLD activation by ABA”
t0“Barley”
Claim · model-written summary
Pertussis toxin inhibited ABA activation of PLD in intact aleurone protoplasts.
- Taxon
- BarleyHordeum vulgaret0
- Organ / stage
- aleurone protoplastse0
- Treatment
- pertussis toxine0
- Outcome
- ability of ABA to activate PLDe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Application of pertussis toxin to intact aleurone protoplasts inhibited the ability of ABA to activate PLD”
t0“Barley”
Claim · model-written summary
Pertussis toxin antagonized ABA down regulation of gibberellic acid stimulated alpha amylase production.
- Taxon
- BarleyHordeum vulgaret0
- Gene
- alpha-amylasee0
- Outcome
- alpha-amylase productione0
- Direction
- antagonizinge0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“antagonizing the ability of ABA to down-regulate gibberellic acid-stimulated alpha-amylase production”
t0“Barley”
Claim · model-written summary
The authors infer an ABA receptor system linked to PLD at the aleurone plasma membrane.
- Taxon
- BarleyHordeum vulgaret0
- Organ / stage
- aleurone plasma membranee0
- Gene
- PLDe0
- Outcome
- PLD activatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results suggest that an ABA receptor system and elements linking it to PLD activation are associated with the aleurone plasma membrane.”
t0“Barley”
Claim · model-written summary
The authors propose G protein mediation of ABA stimulated PLD at the plasma membrane.
- Taxon
- BarleyHordeum vulgaret0
- Organ / stage
- plasma membranee0
- Gene
- G-proteine0
- Outcome
- PLD activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All of these data support the hypothesis that ABA stimulation of PLD activity occurs at the plasma membrane and is mediated by G-protein activity.”
t0“Barley”
The same questions in other papers
pathogen product · molecular: metabolites · Hordeum vulgare (barley)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.