Abstract
The level of mRNAs derived from the plastid-encoded psbD light-responsive promoter (LRP) is controlled by a circadian clock(s) in wheat (Triticum aestivum). The circadian oscillations in the psbD LRP mRNA level persisted for at least three cycles in continuous light and for one cycle in continuous dark, with maxima in subjective morning and minima in subjective early night. In vitro transcription in chloroplast extracts revealed that the circadian cycles in the psbD LRP mRNA level were dominantly attributed to the circadian-regulated transcription of the psbD LRP. The effects of various mutations introduced into the promoter region on the psbD LRP activity in vitro suggest the existence of two positive elements located between -54 and -36, which generally enhance the transcription activity, and an anomalous core promoter structure lacking the functional "-35" element, which plays a crucial role in the circadian fluctuation and light dependency of psbD LRP transcription activity.
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Claims 7
Claim · model-written summary
psbD LRP mRNA is controlled by a circadian clock in wheat.
- Taxon
- wheat (Triticum aestivum)
Triticum aestivume0 - Gene
- psbDe0
- Outcome
- level of mRNAse0
- Qualifier
- circadian clock(s)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The level of mRNAs derived from the plastid-encoded psbD light-responsive promoter (LRP) is controlled by a circadian clock(s) in wheat (Triticum aestivum).”
Claim · model-written summary
psbD LRP mRNA oscillations persisted for three cycles in continuous light.
- Taxon
- WheatTriticum aestivumt0
- Gene
- psbDe0
- Outcome
- circadian oscillations in the psbD LRP mRNA levele0
- Value
- at least three cyclese0
- Qualifier
- continuous lighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The circadian oscillations in the psbD LRP mRNA level persisted for at least three cycles in continuous light and for one cycle in continuous dark, with maxima in subjective morning and minima in subjective early night.”
t0“Wheat”
Claim · model-written summary
psbD LRP mRNA oscillations persisted for one cycle in continuous dark.
- Taxon
- WheatTriticum aestivumt0
- Gene
- psbDe0
- Outcome
- circadian oscillations in the psbD LRP mRNA levele0
- Value
- one cyclee0
- Qualifier
- continuous darke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The circadian oscillations in the psbD LRP mRNA level persisted for at least three cycles in continuous light and for one cycle in continuous dark, with maxima in subjective morning and minima in subjective early night.”
t0“Wheat”
Claim · model-written summary
mRNA maxima occurred in subjective morning and minima in subjective early night.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- maxima in subjective morning and minima in subjective early nighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with maxima in subjective morning and minima in subjective early night”
t0“Wheat”
Claim · model-written summary
Circadian transcription dominantly accounts for psbD LRP mRNA cycling.
- Taxon
- WheatTriticum aestivumt0
- Gene
- psbD LRPe0
- Outcome
- circadian cycles in the psbD LRP mRNA levele0
- Qualifier
- circadian-regulated transcriptione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the circadian cycles in the psbD LRP mRNA level were dominantly attributed to the circadian-regulated transcription of the psbD LRP”
t0“Wheat”
Claim · model-written summary
Two positive elements between -54 and -36 enhance transcription activity.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- transcription activitye0
- Direction
- enhancee0
- Qualifier
- two positive elements located between -54 and -36e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two positive elements located between -54 and -36, which generally enhance the transcription activity”
t0“Wheat”
Claim · model-written summary
An anomalous promoter core lacking a functional -35 element governs circadian and light-dependent transcription.
- Taxon
- WheatTriticum aestivumt0
- Gene
- psbD LRPe0
- Outcome
- circadian fluctuation and light dependency of psbD LRP transcription activitye0
- Qualifier
- an anomalous core promoter structure lacking the functional "-35" elemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an anomalous core promoter structure lacking the functional "-35" element, which plays a crucial role in the circadian fluctuation and light dependency of psbD LRP transcription activity”
t0“Wheat”