Abstract
Late blight of potato ranks among the costliest of crop diseases worldwide. Host resistance offers the best means for controlling late blight, but previously deployed single resistance genes have been short-lived in their effectiveness. The foliar blight resistance gene RB, previously cloned from the wild potato Solanum bulbocastanum, has proven effective in greenhouse tests of transgenic cultivated potato. In this study, we examined the effects of the RB transgene on foliar late blight resistance in transgenic cultivated potato under field production conditions. In a two-year replicated trial, the RB transgene, under the control of its endogenous promoter, provided effective disease resistance in various genetic backgrounds, including commercially prominent potato cultivars, without fungicides. RB copy numbers and transcript levels were estimated with transgene-specific assays. Disease resistance was enhanced as copy numbers and transcript levels increased. The RB gene, like many other disease resistance genes, is constitutively transcribed at low levels. Transgenic potato lines with an estimated 15 copies of the RB transgene maintain high RB transcript levels and were ranked among the most resistant of 57 lines tested. We conclude that even in these ultra-high copy number lines, innate RNA silencing mechanisms have not been fully activated. Our findings suggest resistance-gene transcript levels may have to surpass a threshold before triggering RNA silencing. Strategies for the deployment of RB are discussed in light of the current research.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
The RB transgene provided effective foliar late blight resistance in several potato genetic backgrounds, including commercial cultivars, under field production conditions without fungicides.
- Taxon
- potato
Solanum tuberosume1 - Material
- commercially prominent potato cultivarse1
- Treatment
- the RB transgene, under the control of its endogenous promotere1
- Outcome
- foliar late blight resistancee2
- Qualifier
- without fungicidese1
- Setting
- field production conditionse2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In a two-year replicated trial, the RB transgene, under the control of its endogenous promoter, provided effective disease resistance in various genetic backgrounds, including commercially prominent potato cultivars, without fungicides”
e2“we examined the effects of the RB transgene on foliar late blight resistance in transgenic cultivated potato under field production conditions”
Claim · model-written summary
Disease resistance in transgenic potato was enhanced as RB copy numbers and transcript levels increased.
- Taxon
- potatoSolanum tuberosume0
- Gene
- RBe3
- Outcome
- Disease resistancee4
- Direction
- enhancede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Late blight of potato ranks among the costliest of crop diseases worldwide”
e3“RB copy numbers and transcript levels were estimated with transgene-specific assays”
e4“Disease resistance was enhanced as copy numbers and transcript levels increased”
Claim · model-written summary
Transgenic potato lines with an estimated 15 RB transgene copies were ranked among the most resistant of 57 lines tested.
- Taxon
- potatoSolanum tuberosume5
- Material
- Transgenic potato lines with an estimated 15 copies of the RB transgenee5
- Outcome
- ranked among the most resistant of 57 lines testede5
- Value
- 15 copiese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Transgenic potato lines with an estimated 15 copies of the RB transgene maintain high RB transcript levels and were ranked among the most resistant of 57 lines tested”
Claim · model-written summary
Transgenic potato lines with an estimated 15 RB transgene copies maintained high RB transcript levels.
- Taxon
- potatoSolanum tuberosume5
- Material
- Transgenic potato lines with an estimated 15 copies of the RB transgenee5
- Outcome
- high RB transcript levelse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Transgenic potato lines with an estimated 15 copies of the RB transgene maintain high RB transcript levels and were ranked among the most resistant of 57 lines tested”
Claim · model-written summary
The RB gene is constitutively transcribed at low levels, like many other disease resistance genes.
- Taxon
- potatoSolanum tuberosume0
- Gene
- RBe6
- Outcome
- constitutively transcribede6
- Qualifier
- at low levelse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Late blight of potato ranks among the costliest of crop diseases worldwide”
e6“The RB gene, like many other disease resistance genes, is constitutively transcribed at low levels”
Claim · model-written summary
Even in ultra-high copy number potato lines, innate RNA silencing mechanisms have not been fully activated.
- Taxon
- potatoSolanum tuberosume0
- Outcome
- innate RNA silencing mechanismse7
- Qualifier
- even in these ultra-high copy number linese7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Late blight of potato ranks among the costliest of crop diseases worldwide”
e7“We conclude that even in these ultra-high copy number lines, innate RNA silencing mechanisms have not been fully activated”
Claim · model-written summary
Resistance-gene transcript levels may need to surpass a threshold before triggering RNA silencing in potato.
- Taxon
- potatoSolanum tuberosume0
- Outcome
- RNA silencinge7
- Qualifier
- resistance-gene transcript levels may have to surpass a thresholde7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Late blight of potato ranks among the costliest of crop diseases worldwide”
e7“Our findings suggest resistance-gene transcript levels may have to surpass a threshold before triggering RNA silencing”
The same questions in other papers
transgenic · disease · Solanum tuberosum (potato)Positive and negative results
14 papers in the same direction as this paper · 3 in the opposite direction
- positive
- negative
- negative
14 papers more in this group
See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.