Abstract
PURPOSE: The present investigation is aimed at studies on the effects of gamma rays on in vitro and in vivo damage in Vigna radiata. The parameters studied are germination frequency, seedling injury, isozyme alteration and random amplified polymorphic DNA (RAPD) markers. Results obtained are analyzed in the light of modern applications of radiation damage. MATERIALS AND METHODS: Seeds of Vigna radiata were subjected to gamma irradiation with a dose of 20 - 200 Gy. The percent of seedling damage and frequency of germination were determined. Callus samples were produced in vitro and exposed to gamma rays. The irradiated callus samples were processed to extract total protein, and specifically stained for superoxide dismutase (SOD) and peroxidase isozymes. Total genomic DNA was extracted from irradiated callus samples and subjected to random amplified polymorphic DNA analysis using 23 random decamer primers. RESULTS: Gamma irradiation resulted in retardation in seedling height and decrease in germination frequency in a dose dependent manner. Inhibition assay identified variation in response between different isoforms of SOD on radiation exposure. Changes in peroxidase activity were also observed following irradiation. RAPD analysis showed that new bands appeared in the 20 Gy irradiated sample which in the case of some primers showed similarity with the control. The calli irradiated with 50 Gy and 100 Gy of gamma rays was found to have striking resemblance in banding pattern. Callus irradiated at 200 Gy showed maximum damage. DNA damage as revealed by RAPD analysis was reflected in the appearance of new bands with varying molecular weights. CONCLUSION: New isoforms of SOD appeared after irradiation followed by 24 h recovery. Some isoforms of peroxidase reappeared in calli after 24 h recovery. Results of RAPD analysis indicated that the DNA polymorphism was dose dependent.
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Claims 12
Claim · model-written summary
Gamma irradiation reduced seedling height in a dose-dependent manner.
- Taxon
- Vigna radiata
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Vigna radiatae1 - Treatment
- Gamma irradiatione0
- Outcome
- seedling heighte0
- Direction
- retardatione0
- Qualifier
- dose dependent mannere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Gamma irradiation resulted in retardation in seedling height and decrease in germination frequency in a dose dependent manner”
e1“in Vigna radiata”
Claim · model-written summary
Gamma irradiation decreased germination frequency in a dose-dependent manner.
- Taxon
- Vigna radiataVigna radiatae1
- Treatment
- Gamma irradiatione0
- Outcome
- germination frequencye0
- Direction
- decreasee0
- Qualifier
- dose dependent mannere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gamma irradiation resulted in retardation in seedling height and decrease in germination frequency in a dose dependent manner”
e1“in Vigna radiata”
Claim · model-written summary
SOD isoforms varied in radiation response.
- Taxon
- Vigna radiataVigna radiatae1
- Treatment
- radiation exposuree0
- Gene
- SODe0
- Outcome
- response between different isoforms of SODe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“variation in response between different isoforms of SOD on radiation exposure”
e1“in Vigna radiata”
Claim · model-written summary
Peroxidase activity changed after irradiation.
- Taxon
- Vigna radiataVigna radiatae1
- Treatment
- irradiatione0
- Gene
- peroxidasee0
- Outcome
- peroxidase activitye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Changes in peroxidase activity were also observed following irradiation”
e1“in Vigna radiata”
Claim · model-written summary
New RAPD bands appeared after 20 Gy irradiation.
- Taxon
- Vigna radiataVigna radiatae1
- Treatment
- 20 Gy irradiatede0
- Outcome
- new bands appearede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“new bands appeared in the 20 Gy irradiated sample”
e1“in Vigna radiata”
Claim · model-written summary
Some RAPD primers showed similarity between 20 Gy samples and controls.
- Taxon
- Vigna radiataVigna radiatae1
- Treatment
- 20 Gy irradiatede0
- Outcome
- similarity with the controle0
- Comparator
- the controle0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the 20 Gy irradiated sample which in the case of some primers showed similarity with the control”
e1“in Vigna radiata”
Claim · model-written summary
Calli irradiated with 50 and 100 Gy had strikingly similar banding patterns.
- Taxon
- Vigna radiataVigna radiatae1
- Organ / stage
- callie0
- Treatment
- 50 Gy and 100 Gy of gamma rayse0
- Outcome
- striking resemblance in banding patterne0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The calli irradiated with 50 Gy and 100 Gy of gamma rays was found to have striking resemblance in banding pattern”
e1“in Vigna radiata”
Claim · model-written summary
Callus irradiated at 200 Gy showed maximum damage.
- Taxon
- Vigna radiataVigna radiatae1
- Organ / stage
- Calluse0
- Treatment
- 200 Gye0
- Outcome
- damagee0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus irradiated at 200 Gy showed maximum damage”
e1“in Vigna radiata”
Claim · model-written summary
RAPD revealed DNA damage through new bands of varying molecular weights.
- Taxon
- Vigna radiataVigna radiatae1
- Outcome
- DNA damagee0
- Qualifier
- appearance of new bands with varying molecular weightse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA damage as revealed by RAPD analysis was reflected in the appearance of new bands with varying molecular weights”
e1“in Vigna radiata”
Claim · model-written summary
New SOD isoforms appeared following irradiation and 24-hour recovery.
- Taxon
- Vigna radiataVigna radiatae1
- Treatment
- irradiatione0
- Gene
- SODe0
- Outcome
- New isoforms of SOD appearede0
- Qualifier
- 24 h recoverye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“New isoforms of SOD appeared after irradiation followed by 24 h recovery”
e1“in Vigna radiata”
Claim · model-written summary
Some peroxidase isoforms reappeared in calli after 24-hour recovery.
- Taxon
- Vigna radiataVigna radiatae1
- Organ / stage
- callie0
- Gene
- peroxidasee0
- Outcome
- isoforms of peroxidase reappearede0
- Qualifier
- 24 h recoverye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Some isoforms of peroxidase reappeared in calli after 24 h recovery”
e1“in Vigna radiata”
Claim · model-written summary
RAPD polymorphism depended on irradiation dose.
- Taxon
- Vigna radiataVigna radiatae1
- Treatment
- gamma rayse1
- Outcome
- DNA polymorphisme0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA polymorphism was dose dependent”
e1“gamma rays on in vitro and in vivo damage in Vigna radiata”
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See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.