Abstract
A gene (EGL2) encoding an endo-1,4-beta-glucanase in peas has been cloned as a homologue of EGL1. EGL2 encodes a polypeptide of 506 amino acids, including a 24-mer putative signal polypeptide. The gene product contains a domain conserved in endo-1,4-beta-glucanase (family 9) showing 60% amino acid identity to EGL1. EGL2 mRNA was accumulated only in the elongating regions of pea stems, although EGL1 mRNA was abundant in both elongating and non-elongating tissues. However, the level of EGL2 mRNA was not increased by the treatment with sucrose and auxin in pea segments. These results suggest that the expression of EGL2 either requires the presence of other factors related to the auxin effect or occurs independent of auxin in the elongating pea stems.
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Claims 7
Claim · model-written summary
A pea gene EGL2 was cloned as a homologue of EGL1.
- Taxon
- peas
Lathyrus oleraceuse1 - Gene
- EGL2e1
- Outcome
- homologue of EGL1e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A gene (EGL2) encoding an endo-1,4-beta-glucanase in peas has been cloned as a homologue of EGL1”
Claim · model-written summary
The EGL2 polypeptide has 506 amino acids.
- Taxon
- peasLathyrus oleraceuse2
- Gene
- EGL2e1
- Outcome
- polypeptidee1
- Value
- 506 amino acidse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“EGL2 encodes a polypeptide of 506 amino acids”
e2“A gene (EGL2) encoding an endo-1,4-beta-glucanase in peas has been cloned as a homologue of EGL1”
Claim · model-written summary
The EGL2 gene product shows 60% amino acid identity to EGL1.
- Taxon
- peasLathyrus oleraceuse2
- Outcome
- amino acid identity to EGL1e1
- Value
- 60%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“showing 60% amino acid identity to EGL1”
e2“A gene (EGL2) encoding an endo-1,4-beta-glucanase in peas has been cloned as a homologue of EGL1”
Claim · model-written summary
EGL2 mRNA accumulates only in the elongating regions of pea stems.
- Taxon
- peaLathyrus oleraceuse1
- Organ / stage
- elongating regions of pea stemse1
- Gene
- EGL2e1
- Outcome
- EGL2 mRNAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“EGL2 mRNA was accumulated only in the elongating regions of pea stems”
Claim · model-written summary
EGL1 mRNA is abundant in both elongating and non-elongating pea stem tissues.
- Taxon
- peasLathyrus oleraceuse2
- Organ / stage
- both elongating and non-elongating tissuese1
- Gene
- EGL1e1
- Outcome
- EGL1 mRNAe1
- Qualifier
- abundante1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“EGL1 mRNA was abundant in both elongating and non-elongating tissues”
e2“A gene (EGL2) encoding an endo-1,4-beta-glucanase in peas has been cloned as a homologue of EGL1”
Claim · model-written summary
EGL2 mRNA level in pea segments was not increased by sucrose and auxin treatment.
- Taxon
- peaLathyrus oleraceuse1
- Organ / stage
- pea segmentse1
- Treatment
- sucrose and auxine1
- Gene
- EGL2e1
- Outcome
- level of EGL2 mRNAe1
- Direction
- not increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the level of EGL2 mRNA was not increased by the treatment with sucrose and auxin in pea segments”
Claim · model-written summary
EGL2 expression may either require other factors related to the auxin effect or occur independent of auxin in elongating pea stems (author interpretation).
- Taxon
- peaLathyrus oleraceuse1
- Organ / stage
- elongating pea stemse1
- Gene
- EGL2e1
- Outcome
- expression of EGL2e1
- Qualifier
- requires the presence of other factors related to the auxin effect or occurs independent of auxine1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“These results suggest that the expression of EGL2 either requires the presence of other factors related to the auxin effect or occurs independent of auxin in the elongating pea stems”
The same questions in other papers
class · molecular: expression · Lathyrus oleraceus (pea)Positive and negative results
3 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.