Abstract
To evaluate the reproductive potential of female and male gametes in a haploid plant derived from seedlings of ‘Banpeiyu’ pummelo, we performed crosses between the haploid and several diploid citrus cultivars. In the crosses with the diploid cultivars as pollen parents, no fruit set on the haploid in all cross combinations. However, developed seeds were obtained in four cross combinations, when some monoembryonic diploid cultivars were pollinated with pollen of the haploid. These seeds germinated normally and developed into diploid, vigorous seedlings with large wing leaves, typical of ‘Banpeiyu’ pummelo. Furthermore, the hybridity of a seedling obtained from the cross between ‘Kiyomi’ tangor and the haploid was confirmed by random amplified polymorphic DNA (RAPD) analysis and chromosome composition by chromomycin A3 (CMA) staining. These results suggest that the haploid produced fertile pollen grains (n=9).
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Claims 6
Claim · model-written summary
No fruit set on the haploid pummelo when diploid cultivars supplied pollen.
- Taxon
- Pummelo
Citrus maximat0 - Outcome
- no fruit sete0
- Comparator
- diploid cultivars as pollen parentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the crosses with the diploid cultivars as pollen parents, no fruit set on the haploid in all cross combinations”
t0“Pummelo”
Claim · model-written summary
Developed seeds arose in four crosses when monoembryonic diploids received haploid pollen.
- Taxon
- PummeloCitrus maximat0
- Outcome
- developed seedse0
- Value
- four cross combinationse0
- Qualifier
- monoembryonic diploid cultivars were pollinated with pollen of the haploide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“developed seeds were obtained in four cross combinations, when some monoembryonic diploid cultivars were pollinated with pollen of the haploid”
t0“Pummelo”
Claim · model-written summary
The resulting seeds germinated normally.
- Taxon
- PummeloCitrus maximat0
- Outcome
- germinated normallye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These seeds germinated normally and developed into diploid, vigorous seedlings with large wing leaves”
t0“Pummelo”
Claim · model-written summary
The seeds developed into vigorous diploid seedlings with large wing leaves.
- Taxon
- PummeloCitrus maximat0
- Outcome
- diploid, vigorous seedlings with large wing leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These seeds germinated normally and developed into diploid, vigorous seedlings with large wing leaves”
t0“Pummelo”
Claim · model-written summary
RAPD and chromosome staining confirmed hybridity of a Kiyomi tangor by haploid seedling.
- Taxon
- PummeloCitrus maximat0
- Material
- ‘Kiyomi’ tangore0
- Outcome
- hybridity of a seedlinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the hybridity of a seedling obtained from the cross between ‘Kiyomi’ tangor and the haploid was confirmed by random amplified polymorphic DNA (RAPD) analysis and chromosome composition by chromomycin A3 (CMA) staining”
t0“Pummelo”
Claim · model-written summary
The authors concluded the haploid produced fertile pollen grains.
- Taxon
- PummeloCitrus maximat0
- Outcome
- fertile pollen grainse0
- Value
- n=9e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results suggest that the haploid produced fertile pollen grains (n=9)”
t0“Pummelo”