Abstract
Laminarin-hydrolysing activity developed in the endosperm of tomato (Lycopersicon esculentum) seeds following germination. The enzyme was basic (pI>10) and the apparent molecular mass was estimated to be 35 kDa by SDS-PAGE. It was specific for linear beta-1,3-glucan substrates. Laminarin was hydrolysed by the enzyme to yield a mixture of oligoglucosides, indicating that the enzyme had an endo-action pattern. Thus, the enzyme was identified as beta-1,3- endoglucanase (EC 3.2.1.39). The activity of the enzyme developed in the endosperm after radicle protrusion (germination) had occurred and the enzyme activity was localized exclusively in the micropylar region of the endosperm where the radicle had penetrated. When the lateral endosperm region, where no induction of the enzyme occurred, was wounded (cut or punctured), there was a marked enhancement of beta-1,3-glucanase activity. Thus the post-germinative beta-1, 3-glucanase activity in the micropylar endosperm portion might be brought about by wounding resulting from endosperm rupture by radicle penetration.
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Claims 9
Claim · model-written summary
Laminarin hydrolysing activity developed in tomato seed endosperm following germination.
- Taxon
- tomato
Solanum lycopersicume0 - Organ / stage
- endosperme0
- Outcome
- Laminarin-hydrolysing activitye0
- Direction
- developede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Laminarin-hydrolysing activity developed in the endosperm of tomato (Lycopersicon esculentum) seeds following germination.”
Claim · model-written summary
The enzyme was basic with pI greater than 10.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- basice0
- Value
- pI>10e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The enzyme was basic (pI>10) and the apparent molecular mass was estimated to be 35 kDa by SDS-PAGE.”
t0“tomato”
Claim · model-written summary
Its apparent molecular mass was estimated at 35 kDa.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- apparent molecular masse0
- Value
- 35 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the apparent molecular mass was estimated to be 35 kDa by SDS-PAGE”
t0“tomato”
Claim · model-written summary
The enzyme was specific for linear beta-1,3-glucan substrates.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- specific for linear beta-1,3-glucan substratese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It was specific for linear beta-1,3-glucan substrates.”
t0“tomato”
Claim · model-written summary
Laminarin hydrolysis yielded oligoglucosides, indicating endo-action.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- mixture of oligoglucosidese0
- Qualifier
- endo-action patterne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Laminarin was hydrolysed by the enzyme to yield a mixture of oligoglucosides, indicating that the enzyme had an endo-action pattern.”
t0“tomato”
Claim · model-written summary
The enzyme was identified as beta-1,3-endoglucanase.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- identified as beta-1,3- endoglucanase (EC 3.2.1.39)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thus, the enzyme was identified as beta-1,3- endoglucanase (EC 3.2.1.39).”
t0“tomato”
Claim · model-written summary
Enzyme activity developed after radicle protrusion and only in the micropylar endosperm.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- micropylar region of the endosperme0
- Outcome
- enzyme activity was localized exclusively in the micropylar region of the endosperme0
- Qualifier
- after radicle protrusion (germination) had occurrede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activity of the enzyme developed in the endosperm after radicle protrusion (germination) had occurred and the enzyme activity was localized exclusively in the micropylar region of the endosperm where the radicle had penetrated.”
t0“tomato”
Claim · model-written summary
Wounding lateral endosperm markedly enhanced glucanase activity.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- lateral endosperm regione0
- Treatment
- wounded (cut or punctured)e0
- Outcome
- beta-1,3-glucanase activitye0
- Direction
- marked enhancemente0
- Comparator
- where no induction of the enzyme occurrede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When the lateral endosperm region, where no induction of the enzyme occurred, was wounded (cut or punctured), there was a marked enhancement of beta-1,3-glucanase activity.”
t0“tomato”
Claim · model-written summary
The authors propose wounding during endosperm rupture as the cause of micropylar enzyme activity.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- beta-1, 3-glucanase activitye0
- Qualifier
- wounding resulting from endosperm rupture by radicle penetratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thus the post-germinative beta-1, 3-glucanase activity in the micropylar endosperm portion might be brought about by wounding resulting from endosperm rupture by radicle penetration.”
t0“tomato”
The same questions in other papers
mechanical · physiology: antioxidant enzymes · Solanum lycopersicum (tomato)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.