Abstract
Gluconacetobacter diazotrophicus is an aerobic diazotrophic plant-growth-promoting bacterium isolated from different gramineous plants. We showed that reactive oxygen species (ROS) were produced at early stages of rice root colonization, a typical plant defense response against pathogens. The transcription of the pathogen-related-10 gene of the jasmonic acid (JA) pathway but not of the PR-1 gene of the salicylic acid pathway was activated by the endophytic colonization of rice roots by G. diazotrophicus strain PAL5. Quantitative polymerase chain reaction analyses showed that, at early stages of colonization, the bacteria upregulated the transcript levels of ROS-detoxifying genes such as superoxide dismutase (SOD) and glutathione reductase (GR). To proof the role of ROS-scavenging enzymes in the colonization and interaction process, transposon insertion mutants of the SOD and GR genes of strain PAL5 were constructed. The SOD and GR mutants were unable to efficiently colonize the roots, indicated by the decrease of tightly root-associated bacterial cell counts and endophytic colonization and by fluorescence in situ hybridization analysis. Interestingly, the mutants did not induce the PR-10 of the JA-pathway, probably due to the inability of endophytic colonization. Thus, ROS-scavenging enzymes of G. diazotrophicus strain PAL5 play an important role in the endophytic colonization of rice plants.
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Claims 7
Claim · model-written summary
Reactive oxygen species were produced at early stages of rice root colonization
- Taxon
- rice
Oryza sativae1 - Organ / stage
- early stagese1
- Outcome
- reactive oxygen species (ROS)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“reactive oxygen species (ROS) were produced at early stages of rice root colonization”
Claim · model-written summary
Endophytic colonization by G. diazotrophicus PAL5 activated transcription of the PR-10 gene of the JA pathway in rice
- Taxon
- riceOryza sativae2
- Treatment
- endophytic colonization of rice roots by G. diazotrophicus strain PAL5e2
- Interactor
- G. diazotrophicus strain PAL5Gluconacetobacter diazotrophicuse2symbiont
- Gene
- pathogen-related-10e2
- Outcome
- transcription of the pathogen-related-10 genee2
- Direction
- activatede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“The transcription of the pathogen-related-10 gene of the jasmonic acid (JA) pathway but not of the PR-1 gene of the salicylic acid pathway was activated by the endophytic colonization of rice roots by G. diazotrophicus strain PAL5”
Claim · model-written summary
Endophytic colonization by G. diazotrophicus PAL5 did not activate the PR-1 gene of the salicylic acid pathway in rice
- Taxon
- riceOryza sativae2
- Treatment
- endophytic colonization of rice roots by G. diazotrophicus strain PAL5e2
- Interactor
- G. diazotrophicus strain PAL5Gluconacetobacter diazotrophicuse2symbiont
- Gene
- PR-1e2
- Outcome
- PR-1 genee2
- Direction
- no_changeinferrede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“The transcription of the pathogen-related-10 gene of the jasmonic acid (JA) pathway but not of the PR-1 gene of the salicylic acid pathway was activated by the endophytic colonization of rice roots by G. diazotrophicus strain PAL5”
Claim · model-written summary
Bacteria upregulated transcript levels of superoxide dismutase and glutathione reductase at early stages of colonization in rice
- Taxon
- RiceOryza sativat0
- Organ / stage
- early stages of colonizatione3
- Gene
- superoxide dismutase (SOD) and glutathione reductase (GR)e3
- Outcome
- transcript levels of ROS-detoxifying genese3
- Direction
- upregulatede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Endophytic Colonization of Rice Roots”
e3“Quantitative polymerase chain reaction analyses showed that, at early stages of colonization, the bacteria upregulated the transcript levels of ROS-detoxifying genes such as superoxide dismutase (SOD) and glutathione reductase (GR)”
Claim · model-written summary
SOD and GR mutants of the endophyte colonized rice roots less efficiently, with decreased tightly root-associated cell counts and endophytic colonization
- Taxon
- RiceOryza sativat0
- Treatment
- SOD and GR mutantse4
- Gene
- SOD and GRe4
- Outcome
- tightly root-associated bacterial cell counts and endophytic colonizatione4
- Direction
- decreasee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Endophytic Colonization of Rice Roots”
e4“The SOD and GR mutants were unable to efficiently colonize the roots, indicated by the decrease of tightly root-associated bacterial cell counts and endophytic colonization and by fluorescence in situ hybridization analysis”
Claim · model-written summary
SOD and GR mutants did not induce the PR-10 gene of the JA pathway in rice
- Taxon
- RiceOryza sativat0
- Treatment
- mutantse5
- Gene
- PR-10e5
- Outcome
- PR-10 of the JA-pathwaye5
- Direction
- no_changeinferrede5
- Qualifier
- probably due to the inability of endophytic colonizatione5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Endophytic Colonization of Rice Roots”
e5“the mutants did not induce the PR-10 of the JA-pathway, probably due to the inability of endophytic colonization”
Claim · model-written summary
ROS-scavenging enzymes of G. diazotrophicus PAL5 play an important role in endophytic colonization of rice
- Taxon
- riceOryza sativae6
- Interactor
- G. diazotrophicus strain PAL5Gluconacetobacter diazotrophicuse6symbiont
- Gene
- ROS-scavenging enzymese6
- Outcome
- endophytic colonizatione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Endophytic Colonization of Rice Roots”
e6“ROS-scavenging enzymes of G. diazotrophicus strain PAL5 play an important role in the endophytic colonization of rice plants”
The same questions in other papers
Gluconacetobacter diazotrophicus · molecular: expression · Oryza sativa (rice)Positive and negative results
1 in the opposite direction
- positive
infection · molecular: expression · Oryza sativa (rice)Positive and negative results
15 papers in the same direction as this paper · 41 in the opposite direction
- positive
- positive
- positive
53 papers more in this group
See all 57 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.