Abstract
"Transgenic mango (Mangifera indica L.) plants were regenerated from somatic embryos inoculated with a wild Agrobacterium rhizogenes strain. The bacteria were grown in Luria Bertani medium supplemented or not with acetosyringone until an optical density of 1.0-1.5 was reached. Incubation time of somatic embryos from the Kent, Haden and Madame Francis varieties in bacterial suspension was 1h, 15 and 5min. Somatic embryos were transferred into Gamborg Miller Ojima semisolid medium for 48h, and finally placed on the same medium containing cefotaxime/carbencillin, in darkness at 27 ±1°C. In the first treatment, A. rhizogenes was eliminated after 20 washings with cefotaxime/carbencillin, only in the Kent variety; while 1-5 washes were needed for the other treatments. There were no differences between preculture treatments of A. rhizogenes with or without acetosyringone. After infection embryos formed callus, then secondary embryos and, finally, plants were regenerated but hairy roots were not induced. The presence of rolB gene in leaf tissue was confirmed by PCR. An expected band of 720bp, corresponding to rol B gen amplification, was obtained only in the transformed plants. An efficient protocol has been developed for successful production of transgenic somatic embryos and plants of the Kent variety using A. rhizogenes. Twenty transgenic clones per 0.5g of inoculated tissues with 80% embryo survival were produced. The results constitute the first report of transgenic mango plants mediated by A. rhizogenes as an alternative tool for breeding improvement of this crop."
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Claims 6
Claim · model-written summary
The Kent variety required 20 cefotaxime/carbencillin washes to eliminate A. rhizogenes in the first treatment.
- Taxon
- mango
_from_India.jpg?width=60)
Mangifera indicat0 - Material
- Kente0
- Treatment
- cefotaxime/carbencilline0
- Outcome
- A. rhizogenes was eliminatede0
- Value
- 20 washingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_from_India.jpg?width=500)
Verbatim
e0“In the first treatment, A. rhizogenes was eliminated after 20 washings with cefotaxime/carbencillin, only in the Kent variety”
t0“mango”
Claim · model-written summary
One to five washes eliminated A. rhizogenes in the other treatments.
- Taxon
- mangoMangifera indicat0
- Outcome
- washes were needede0
- Value
- 1-5 washese0
- Qualifier
- other treatmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while 1-5 washes were needed for the other treatments”
t0“mango”
Claim · model-written summary
Acetosyringone preculture did not produce differences between treatments.
- Taxon
- mangoMangifera indicat0
- Treatment
- acetosyringonee0
- Outcome
- preculture treatmentse0
- Direction
- no differencese0
- Comparator
- without acetosyringonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There were no differences between preculture treatments of A. rhizogenes with or without acetosyringone”
t0“mango”
Claim · model-written summary
Infected embryos formed callus and secondary embryos before plant regeneration, without inducing hairy roots.
- Taxon
- mangoMangifera indicat0
- Organ / stage
- embryose0
- Outcome
- callus, then secondary embryos and, finally, plants were regeneratede0
- Qualifier
- hairy roots were not inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After infection embryos formed callus, then secondary embryos and, finally, plants were regenerated but hairy roots were not induced”
t0“mango”
Claim · model-written summary
PCR confirmed the rolB gene in transformed mango leaf tissue, with an expected 720bp band only in transformed plants.
- Taxon
- mangoMangifera indicat0
- Material
- transformed plantse0
- Organ / stage
- leaf tissuee0
- Gene
- rolBe0
- Outcome
- presence of rolB genee0
- Value
- 720bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of rolB gene in leaf tissue was confirmed by PCR. An expected band of 720bp, corresponding to rol B gen amplification, was obtained only in the transformed plants”
t0“mango”
Claim · model-written summary
The protocol produced 20 transgenic clones per 0.5g inoculated tissue with 80% embryo survival in Kent mango.
- Taxon
- mangoMangifera indicat0
- Material
- Kente1
- Outcome
- transgenic clonese0
- Value
- Twenty transgenic clones per 0.5ge0
- Qualifier
- 80% embryo survivale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Twenty transgenic clones per 0.5g of inoculated tissues with 80% embryo survival were produced”
e1“transgenic somatic embryos and plants of the Kent variety”
t0“mango”