Abstract
Three tomato (Lycopersicon esculentum Mill.) cultivars were studied to determine the effect of nine dark-light regimes on anther callus initiation and growth. Prophase I (leptotene) stage anthers of `L-680A', `Licato', and `Ailsa Craig' were plated on Doy's basal medium 1 and provided with 2 to 10 weeks of dark treatment. After each dark period, each plate was transferred to a 16-hour photoperiod for the duration of the 10-week experiment. At this time, the number of anthers producing calli and the diameter of the calli produced were recorded. Callus number and diameter increased as the dark period duration increased. Callus diameter peaked at 8 weeks of dark treatment for `Ailsa Craig' and `Licato', whereas `L-680A' calli continued to grow over the entire 10-week dark treatment. Although the number and size of callus may continue to increase past 8 weeks of dark incubation, callus quality and appearance decreased noticeably during this period. For each additional week of dark period exposure, 7% more of the plated anthers produce callus, and callus diameter increases by 0.27 mm (12% of total growth). -
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Claims 7
Claim · model-written summary
Callus number and diameter increased with dark period duration.
- Taxon
- Tomato
Solanum lycopersicumt0 - Organ / stage
- Calluse0
- Treatment
- dark period duratione0
- Outcome
- Callus number and diametere0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tomato”
e0“Callus number and diameter increased as the dark period duration increased”
Claim · model-written summary
Callus diameter peaked after eight dark weeks for Ailsa Craig.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- Ailsa Craige0
- Outcome
- Callus diametere0
- Direction
- peakede0
- Value
- 8 weekse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tomato”
e0“Callus diameter peaked at 8 weeks of dark treatment for `Ailsa Craig' and `Licato'”
Claim · model-written summary
Callus diameter peaked after eight dark weeks for Licato.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- Licatoe0
- Outcome
- Callus diametere0
- Direction
- peakede0
- Value
- 8 weekse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tomato”
e0“Callus diameter peaked at 8 weeks of dark treatment for `Ailsa Craig' and `Licato'”
Claim · model-written summary
L-680A calli continued to grow throughout ten weeks of dark treatment.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- L-680Ae0
- Outcome
- callie0
- Direction
- continued to growe0
- Value
- 10-weeke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tomato”
e0“`L-680A' calli continued to grow over the entire 10-week dark treatment”
Claim · model-written summary
Callus quality and appearance decreased after eight dark weeks.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- dark incubatione1
- Outcome
- callus quality and appearancee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tomato”
e0“callus quality and appearance decreased noticeably during this period”
e1“8 weeks of dark incubation”
Claim · model-written summary
Each additional dark week yielded callus from 7% more anthers.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- additional week of dark period exposuree0
- Outcome
- plated anthers produce calluse0
- Direction
- moree0
- Value
- 7%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tomato”
e0“For each additional week of dark period exposure, 7% more of the plated anthers produce callus”
Claim · model-written summary
Callus diameter rose by 0.27 mm per additional dark week.
- Taxon
- TomatoSolanum lycopersicumt0
- Organ / stage
- calluse0
- Outcome
- callus diametere0
- Direction
- increasese0
- Value
- 0.27 mme0
- Qualifier
- 12% of total growthe0
Not reportedTreatment
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tomato”
e0“callus diameter increases by 0.27 mm (12% of total growth)”
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See all 226 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.