Abstract
The study on the effect of gamma irradiation on in-vitro shoot growth of chrysanthemum cv. Yellow Puma has been carried out. The aim of the study was to observe genetic variability of shoot growth caused by gamma irradiation. Shoot explants with four leaves were irradiated by gamma with dose of 10, 15 and 20 Gy with 3 replications at each of dose. The irradiated shoot explants were then transferred into fresh MS solid medium and placed in a growth room. Observation was performed on number of leaves and branches on M1V0 generation, while plantlets height and number of branches were observed a M1V1 generation. Number of survival plantlets and multiplication rate on three subsequent subcultures were observed as well. Results showed that gamma rays with dose of 20 Gy inhibited growth of leaves as much as 50% compared to control (shoots without irradiation), and branches 73.7% in three weeks. Observation on multiplication rate at M1V1 generation showed that gamma irradiation with dose of 10 Gy promoted multiplication rate as much as 10% higher than control. It can be concluded that in vitro mutagenesis using gamma iradiation with dose of 10 to 15 Gy can be used for inducing genetic variability of chrysanthemum cv. Yellow Puma. Received: 18 December 2008; Revised: 10 August 2010; Accepted: 30 August 2010
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Claims 4
Claim · model-written summary
Gamma irradiation at 20 Gy inhibited leaf growth of in vitro chrysanthemum shoots by as much as 50% compared with unirradiated shoots.
- Taxon
- chrysanthemum
.jpeg?width=60)
Chrysanthemume0 - Material
- cv. Yellow Pumae0
- Treatment
- gamma rays with dose of 20 Gye1
- Outcome
- growth of leavese1
- Direction
- inhibitede1
- Value
- 50%e1
- Comparator
- control (shoots without irradiation)e1
- Setting
- in-vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpeg?width=500)
Verbatim
e0“in-vitro shoot growth of chrysanthemum cv. Yellow Puma”
e1“gamma rays with dose of 20 Gy inhibited growth of leaves as much as 50% compared to control (shoots without irradiation)”
Claim · model-written summary
Gamma irradiation at 20 Gy inhibited branch growth of in vitro chrysanthemum shoots by 73.7% over three weeks.
- Taxon
- chrysanthemumChrysanthemume0
- Material
- cv. Yellow Pumae0
- Treatment
- gamma rays with dose of 20 Gye2
- Outcome
- branchese2
- Direction
- inhibitede2
- Value
- 73.7%e2
- Qualifier
- in three weekse2
- Comparator
- control (shoots without irradiation)e2
- Setting
- in-vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in-vitro shoot growth of chrysanthemum cv. Yellow Puma”
e2“gamma rays with dose of 20 Gy inhibited growth of leaves as much as 50% compared to control (shoots without irradiation), and branches 73.7% in three weeks”
Claim · model-written summary
Gamma irradiation at 10 Gy raised the multiplication rate at the M1V1 generation by as much as 10% over control.
- Taxon
- chrysanthemumChrysanthemume0
- Material
- cv. Yellow Pumae0
- Treatment
- gamma irradiation with dose of 10 Gye3
- Outcome
- multiplication ratee3
- Direction
- promotede3
- Value
- 10%e3
- Qualifier
- at M1V1 generatione3
- Comparator
- controle3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in-vitro shoot growth of chrysanthemum cv. Yellow Puma”
e3“Observation on multiplication rate at M1V1 generation showed that gamma irradiation with dose of 10 Gy promoted multiplication rate as much as 10% higher than control”
Claim · model-written summary
In vitro mutagenesis with 10 to 15 Gy gamma irradiation can be used to induce genetic variability in chrysanthemum cv. Yellow Puma.
- Taxon
- chrysanthemumChrysanthemume0
- Material
- cv. Yellow Pumae0
- Treatment
- gamma iradiation with dose of 10 to 15 Gye4
- Outcome
- inducing genetic variabilitye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in-vitro shoot growth of chrysanthemum cv. Yellow Puma”
e4“It can be concluded that in vitro mutagenesis using gamma iradiation with dose of 10 to 15 Gy can be used for inducing genetic variability of chrysanthemum cv. Yellow Puma”
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