Abstract
Jatropha curcas, a plant with great biodiesel potential is also used to reduce the population of whiteflies, Bemisia tabaci on cassava fields when planted as a hedge. We therefore, investigated the transmission of African cassava mosaic virus (ACMV) by the whitefly vector from cassava to seedlings of ten accessions of J. curcas as part of a wider investigation on the possible role of J. curcas as an alternative host of ACMV. Transmission tests were conducted in insect-proof cages using adult B. tabacicollected from ACMV-infected cassava in the field, at a rate of three adult whiteflies per J. curcas seedling and a transmission feeding period of four days. Twenty one (21) days after the infestation, leaf samples from individual plants of the ten J. curcasaccessions were tested for the presence of ACMV by the polymerase chain reaction (PCR) and the double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), using the monoclonal antibodies SCRI 33. DAS-ELISA detected ACMV in five out of the ten J. curcas accessions while PCR detected it in eight of the ten accessions. Furthermore, 18 out of the 35 Nicotiana benthamiana indicator plants mechanically inoculated with sap from symptomatic J. curcas seedlings produced symptoms typical of ACMV infection. This indicates that J. curcas is a likely host of ACMV and it may in turn, be able to infect cassava, and presumably other ACMV-susceptible hosts, in the presence of the vector. Key words: Jatropha curcas accessions, controlled transmission, mechanical inoculation, enzyme-linked immunosorbent assay, African cassava mosaic virus(ACMV)-susceptible hosts.
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Claims 5
Claim · model-written summary
DAS-ELISA detected ACMV in five of ten Jatropha accessions.
- Taxon
- Jatropha curcas
_tree_at_Samedha.jpg?width=60)
Jatropha curcase1 - Material
- J. curcas accessionse0
- Interactor
- ACMVe0pathogen
- Outcome
- detected ACMVe0
- Value
- five out of the tene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tree_at_Samedha.jpg?width=500)
Verbatim
e0“DAS-ELISA detected ACMV in five out of the ten J. curcas accessions”
e1“Jatropha curcas, a plant with great biodiesel potential”
Claim · model-written summary
PCR detected ACMV in eight of ten Jatropha accessions.
- Taxon
- Jatropha curcasJatropha curcase2
- Interactor
- ACMVe1pathogen
- Outcome
- detected ite0
- Value
- eight of the tene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PCR detected it in eight of the ten accessions.”
e1“DAS-ELISA detected ACMV in five out of the ten J. curcas accessions”
e2“Jatropha curcas, a plant with great biodiesel potential”
Claim · model-written summary
Sap from symptomatic Jatropha seedlings induced typical ACMV symptoms in 18 of 35 Nicotiana benthamiana indicators.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- mechanically inoculated with sap from symptomatic J. curcas seedlingse0
- Interactor
- ACMVe0pathogen
- Outcome
- symptoms typical of ACMV infectione0
- Value
- 18 out of the 35e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“18 out of the 35 Nicotiana benthamiana indicator plants mechanically inoculated with sap from symptomatic J. curcas seedlings produced symptoms typical of ACMV infection.”
e1“Jatropha curcas, a plant with great biodiesel potential”
Claim · model-written summary
The authors interpret Jatropha curcas as a likely host of ACMV.
- Taxon
- Jatropha curcasJatropha curcase1
- Interactor
- ACMVe0pathogen
- Outcome
- likely host of ACMVe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This indicates that J. curcas is a likely host of ACMV”
e1“Jatropha curcas, a plant with great biodiesel potential”
Claim · model-written summary
The authors suggest Jatropha curcas may infect cassava and other susceptible hosts when the vector is present.
- Taxon
- Jatropha curcasJatropha curcase1
- Interactor
- ACMVe2pathogen
- Outcome
- be able to infect cassava, and presumably other ACMV-susceptible hostse0
- Qualifier
- in the presence of the vectore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it may in turn, be able to infect cassava, and presumably other ACMV-susceptible hosts, in the presence of the vector.”
e1“Jatropha curcas, a plant with great biodiesel potential”
e2“African cassava mosaic virus (ACMV)”
The same questions in other papers
inoculation · disease · Nicotiana benthamianaPositive and negative results
31 papers in the same direction as this paper · 8 in the opposite direction
- negative
- positive
- positive
36 papers more in this group
See all 40 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.