Paper · 2010 · Biologia Plantarum

High efficiency organogenesis and analysis of genetic stability of the regenerants in Solanum melongena

Y. Xing; Y. Yu; X. Luo; et al.

3
claims
7
verbatim quotes
35
citations
2
research areas
✓
Open access

Abstract

A novel protocol for plant regeneration from cotyledon explants of eggplant (Solanum melongena) reducing concentration of sucrose was established. The most efficient bud induction medium consisted of Murashige and Skoog (MS) medium supplemented with 2.0 mg dm-3 zeatin, 0.1 mg dm-3 indoleacetic acid and 10 g dm-3 sucrose. After 15 d, the shoot buds were fragmented and transferred to the shoot elongation MS supplemented with 1.0-2.0 mg dm-3 gibberellic acid and 4.0-8.0 mg dm-3 AgNO3, which promoted shoots elongation. The genetic stability of the regenerated plants was analyzed by flow cytometry, RAPD and SSR molecular markers. The results indicated that almost no somaclonal variation was detected among the regenerants.

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Claims 3

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The most efficient bud induction medium for eggplant cotyledon explants was MS medium with 2.0 mg/dm3 zeatin, 0.1 mg/dm3 indoleacetic acid and 10 g/dm3 sucrose.

Taxon
Solanum melongenaSolanum melongenat0
Organ / stage
cotyledon explantse0
Treatment
Murashige and Skoog (MS) medium supplemented with 2.0 mg dm-3 zeatin, 0.1 mg dm-3 indoleacetic acid and 10 g dm-3 sucrosee1
Outcome
bud inductione1
Direction
most efficiente1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum melongena
Solanum melongena · TaxonBeraDigle · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Solanum melongena”

✓ located verbatim in the source · title, characters 86–103 · show in text ↑
e0

“A novel protocol for plant regeneration from cotyledon explants of eggplant (Solanum melongena) reducing concentration of sucrose was established”

✓ located verbatim in the source · abstract, characters 0–145 · show in text ↑
e1

“The most efficient bud induction medium consisted of Murashige and Skoog (MS) medium supplemented with 2.0 mg dm-3 zeatin, 0.1 mg dm-3 indoleacetic acid and 10 g dm-3 sucrose”

✓ located verbatim in the source · abstract, characters 147–321 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Shoot elongation on MS medium with gibberellic acid and AgNO3 promoted shoot elongation in eggplant.

Taxon
Solanum melongenaSolanum melongenat0
Organ / stage
shoot budse2
Treatment
shoot elongation MS supplemented with 1.0-2.0 mg dm-3 gibberellic acid and 4.0-8.0 mg dm-3 AgNO3e2
Outcome
shoots elongatione2
Direction
promotede2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum melongena
Solanum melongena · TaxonBeraDigle · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Solanum melongena”

✓ located verbatim in the source · title, characters 86–103 · show in text ↑
e2

“After 15 d, the shoot buds were fragmented and transferred to the shoot elongation MS supplemented with 1.0-2.0 mg dm-3 gibberellic acid and 4.0-8.0 mg dm-3 AgNO3, which promoted shoots elongation”

✓ located verbatim in the source · abstract, characters 323–519 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

Almost no somaclonal variation was detected among the regenerated eggplant plants.

Taxon
Solanum melongenaSolanum melongenat0
Material
regenerantse3
Outcome
somaclonal variatione3
Qualifier
almost noe3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum melongena
Solanum melongena · TaxonBeraDigle · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Solanum melongena”

✓ located verbatim in the source · title, characters 86–103 · show in text ↑
e3

“The results indicated that almost no somaclonal variation was detected among the regenerants”

✓ located verbatim in the source · abstract, characters 633–725 · show in text ↑

Gibberellic acid · growth · Solanum melongena (eggplant)Positive and negative results

28 papers: 26 positive, 2 negative or no effect

25 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. negative
  3. positive

24 papers more in this group

See all 28 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.