Abstract
The effect of protease inhibitors on the response of gibberellic acid-treated barley aleurone layers to ethylene was examined. In the absence of protease inhibitors, ethylene plus gibberellic acid initially increased the production of amylase activity relative to layers incubated with gibberellic acid alone. Exposure to ethylene plus gibberellic acid for 48 hours or longer, however, led to depressed levels of amylase activity compared to samples incubated with gibberellic acid in hydrocarbon-free air.The direct assay of proteolytic activity revealed a small increase in activity in response to ethylene. The significance of this response was probed further by including inhibitors of barley proteases in the incubation medium. When potassium bromate was introduced, ethylene did not cause any alteration in amylase activity compared to samples incubated in hydrocarbon-free air. However, in the presence of N-ethylmaleimide, ethylene treatment induced a 52% increase in amylase activity recovered from samples after 48 hours. These results suggest that proteases contribute to the loss of amylase activity in response to ethylene and thus alter the apparent effect of ethylene on amylase synthesis. The effect of protease inhibitors on other hydrolases is also discussed.During the incubation period, the pH of the medium declined significantly. However, ethylene had no effect on the extent of this decline.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Ethylene plus gibberellic acid initially raised amylase activity relative to gibberellic acid alone.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaret0 - Treatment
- ethylene plus gibberellic acide0
- Outcome
- amylase activitye0
- Direction
- increasede0
- Comparator
- gibberellic acid alonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“ethylene plus gibberellic acid initially increased the production of amylase activity relative to layers incubated with gibberellic acid alone”
t0“Barley Aleurone Layers”
Claim · model-written summary
After at least 48 hours, ethylene plus gibberellic acid depressed amylase activity relative to gibberellic acid in clean air.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- ethylene plus gibberellic acide0
- Outcome
- amylase activitye0
- Direction
- depressede0
- Comparator
- gibberellic acid in hydrocarbon-free aire0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exposure to ethylene plus gibberellic acid for 48 hours or longer, however, led to depressed levels of amylase activity compared to samples incubated with gibberellic acid in hydrocarbon-free air”
t0“Barley Aleurone Layers”
Claim · model-written summary
Ethylene slightly increased proteolytic activity.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- activitye0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“small increase in activity in response to ethylene”
t0“Barley Aleurone Layers”
Claim · model-written summary
With potassium bromate, ethylene did not alter amylase activity.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- ethylenee0
- Outcome
- amylase activitye0
- Direction
- did not cause any alteratione0
- Comparator
- hydrocarbon-free aire0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When potassium bromate was introduced, ethylene did not cause any alteration in amylase activity compared to samples incubated in hydrocarbon-free air”
t0“Barley Aleurone Layers”
Claim · model-written summary
With N-ethylmaleimide, ethylene increased recovered amylase activity by 52% after 48 hours.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- amylase activitye0
- Direction
- increasee0
- Value
- 52%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the presence of N-ethylmaleimide, ethylene treatment induced a 52% increase in amylase activity recovered from samples after 48 hours”
t0“Barley Aleurone Layers”
Claim · model-written summary
Proteases may contribute to ethylene-associated loss of amylase activity.
- Taxon
- barleyHordeum vulgaret0
- Gene
- proteasese0
- Outcome
- loss of amylase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“proteases contribute to the loss of amylase activity in response to ethylene”
t0“Barley Aleurone Layers”
Claim · model-written summary
Incubation medium pH declined significantly.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- pH of the mediume0
- Direction
- declinede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pH of the medium declined significantly”
t0“Barley Aleurone Layers”
Claim · model-written summary
Ethylene did not affect the medium pH decline.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- extent of this declinee0
- Direction
- no effecte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ethylene had no effect on the extent of this decline”
t0“Barley Aleurone Layers”
The same questions in other papers
Gibberellic acid · physiology: enzyme activity · Hordeum vulgare (barley)Positive and negative results
13 papers in the same direction as this paper · 3 in the opposite direction
- no effect
- negative
- no effect
13 papers more in this group
See all 17 papers →ethene · physiology: enzyme activity · Hordeum vulgare (barley)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.