Abstract
A new micropropagation system for Lycium barbarum (L.) was developed using root explants as starting material. Callus can be produced from root explants on Murashige and Skoog (MS) medium containing 0.2 mg dm-3 2,4-dichlorophenoxyacetic acid. After three subcultures on the same medium, callus was then transferred onto the MS medium supplemented with 500 mg dm-3 lactalbumin hydrolysate to induce somatic embryogenesis (SE). After 20 d, about 60 somatic embryos per 0.25 g(f.m.) of embryogenic callus were obtained but only about 10 % of embryos converted into plantlets. After acclimated in the greenhouse, all of the randomly selected plantlets had survived and were similar phenotypically to zygotic seedlings. In addition, the effects of irradiance, photoperiod, growth regulators, explant age and cold treatment on SE of root-derived callus were evaluated.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Root explants produced callus on MS medium with 2,4-dichlorophenoxyacetic acid.
- Taxon
- Lycium barbarum
Lycium barbarumt0 - Organ / stage
- root explantse0
- Outcome
- Callus can be producede0
Not reportedMaterialGeneInteractorInteractor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus can be produced from root explants on Murashige and Skoog (MS) medium containing 0.2 mg dm-3 2,4-dichlorophenoxyacetic acid.”
t0“Lycium barbarum”
Claim · model-written summary
About 60 somatic embryos were obtained per 0.25 g embryogenic callus after 20 days.
- Taxon
- Lycium barbarumLycium barbarumt0
- Outcome
- somatic embryose0
- Value
- about 60 somatic embryos per 0.25 g(f.m.)e0
Not reportedMaterialGeneOrgan / stageInteractorInteractor roleTreatmentComparatorSettingDirectionQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After 20 d, about 60 somatic embryos per 0.25 g(f.m.) of embryogenic callus were obtained”
t0“Lycium barbarum”
Claim · model-written summary
Only about 10% of embryos converted into plantlets.
- Taxon
- Lycium barbarumLycium barbarumt0
- Outcome
- embryos converted into plantletse0
- Value
- about 10 %e0
Not reportedMaterialGeneOrgan / stageInteractorInteractor roleTreatmentComparatorSettingDirectionQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only about 10 % of embryos converted into plantlets”
t0“Lycium barbarum”
Claim · model-written summary
All randomly selected plantlets survived greenhouse acclimation.
- Taxon
- Lycium barbarumLycium barbarumt0
- Outcome
- all of the randomly selected plantlets had survivede0
- Setting
- greenhousee0
Not reportedMaterialGeneOrgan / stageInteractorInteractor roleTreatmentComparatorDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After acclimated in the greenhouse, all of the randomly selected plantlets had survived”
t0“Lycium barbarum”
Claim · model-written summary
Acclimated plantlets were phenotypically similar to zygotic seedlings.
- Taxon
- Lycium barbarumLycium barbarumt0
- Outcome
- similar phenotypically to zygotic seedlingse0
Not reportedMaterialGeneOrgan / stageInteractorInteractor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“were similar phenotypically to zygotic seedlings”
t0“Lycium barbarum”