Paper · 1973 · Crop Science

Nitrate Reductase Activity in Corn Leaves as Related to Yields of Grain and Grain Protein1

E. L. Deckard; R. J. Lambert; R. H. Hageman

11
claims
22
verbatim quotes
91
citations
2
research areas
—
Not open access

Abstract

The objective was to determine whether a relationship between nitrate reductase activity (NRA) and grain yields and grain protein could be detected for six corn ( Zea mays L.) hybrids grown under field conditions with supplemental N and irrigation. NRA was expressed as seasonal input of reduced N (kg N ha‐ 1 ) and as seasonal averages of activity (µmoles N, g fresh wt‐ 1 , hr‐ 1 ). The treatment variables were plant density (59,300 and 79,000 plants ha‐ 1 ) and tune of application (14 days after tassel initiation, at tassel emergence, and at silking) of nitrate‐N (337 kg N ha‐ 1 ). Each supplemental N treatment, regardless of time of application, significantly increased the percent of grain protein for all genotypes and amount of grain protein ha‐ 1 for all but one hybrid (No. 3). In contrast, the supplemental N treatments did not cause significant increases in grain yields within a given genotype. There were significant differences in grain yield and grain protein among the genotypes. The treatments increased leaf blade nitrate concentration which resulted in a corresponding increase in NRA. However, each successive N application was less effective in increasing and maintaining leaf nitrate content and enzyme activity. None of the hybrids were able to maintain a high concentration of leaf blade nitrate after silking, even though massive amounts of nitrate were applied to the soil. Compared to all other hybrids, hybrid No. 4 maintained a slightly higher level of nitrate concentration and enzyme activity in the leaves during the post‐anthesis stage. NRA of the total leaf canopy, expressed as seasonal averages or converted into seasonal input of reduced N, showed a significant positive correlation with grain protein (kg N/ha), grain yield, and total reduced N in the vegetative material (above ground) and grain and stover at maturity. The highest correlations between NRA and yields of grain and grain protein (kg N/ha) were obtained during the stages of ear initiation and development. This suggests that a minimal number (1 to 3) of samplings would be as effective as the laborious full season samplings (12 or more dates) in selecting individual plants or varieties that have a high potential for grain yields or grain protein production.

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Claims 11

Associationobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Nitrate reductase activity of the total leaf canopy correlated positively with grain protein, grain yield and total reduced N in corn.

Material
six corn ( Zea mays L.) hybridse0
Outcome
grain protein (kg N/ha), grain yield, and total reduced Ne1
Direction
positivee1
Setting
field conditionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“six corn ( Zea mays L.) hybrids grown under field conditions with supplemental N and irrigation”

✓ located verbatim in the source · abstract, characters 152–247 · show in text ↑
e1

“NRA of the total leaf canopy, expressed as seasonal averages or converted into seasonal input of reduced N, showed a significant positive correlation with grain protein (kg N/ha), grain yield, and total reduced N in the vegetative material (above ground) and grain and stover at maturity”

✓ located verbatim in the source · abstract, characters 1575–1862 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Supplemental N treatments significantly increased the percent of grain protein in all genotypes.

Treatment
supplemental N treatmente2
Outcome
percent of grain proteine2
Direction
significantly increasede2
Qualifier
regardless of time of applicatione2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“six corn ( Zea mays L.) hybrids grown under field conditions with supplemental N and irrigation”

✓ located verbatim in the source · abstract, characters 152–247 · show in text ↑
e2

“Each supplemental N treatment, regardless of time of application, significantly increased the percent of grain protein for all genotypes”

✓ located verbatim in the source · abstract, characters 589–725 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Supplemental N treatments did not significantly increase grain yield within a given genotype.

Treatment
supplemental N treatmentse3
Outcome
grain yieldse3
Direction
did not cause significant increasese3
Qualifier
within a given genotypee3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“six corn ( Zea mays L.) hybrids grown under field conditions with supplemental N and irrigation”

✓ located verbatim in the source · abstract, characters 152–247 · show in text ↑
e3

“In contrast, the supplemental N treatments did not cause significant increases in grain yields within a given genotype”

✓ located verbatim in the source · abstract, characters 792–910 · show in text ↑
Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Grain yield and grain protein differed significantly among corn genotypes.

Material
genotypese4
Outcome
grain yield and grain proteine4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“six corn ( Zea mays L.) hybrids grown under field conditions with supplemental N and irrigation”

✓ located verbatim in the source · abstract, characters 152–247 · show in text ↑
e4

“There were significant differences in grain yield and grain protein among the genotypes”

✓ located verbatim in the source · abstract, characters 912–999 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Treatments increased leaf blade nitrate concentration in corn.

Treatment
The treatmentse5
Outcome
leaf blade nitrate concentratione5
Direction
increasede5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“six corn ( Zea mays L.) hybrids grown under field conditions with supplemental N and irrigation”

✓ located verbatim in the source · abstract, characters 152–247 · show in text ↑
e5

“The treatments increased leaf blade nitrate concentration which resulted in a corresponding increase in NRA”

✓ located verbatim in the source · abstract, characters 1001–1108 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Treatments caused a corresponding increase in nitrate reductase activity in corn leaves.

Treatment
The treatmentse5
Outcome
NRAe5
Direction
corresponding increasee5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“six corn ( Zea mays L.) hybrids grown under field conditions with supplemental N and irrigation”

✓ located verbatim in the source · abstract, characters 152–247 · show in text ↑
e5

“The treatments increased leaf blade nitrate concentration which resulted in a corresponding increase in NRA”

✓ located verbatim in the source · abstract, characters 1001–1108 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

Each successive N application was less effective in increasing and maintaining leaf nitrate content and enzyme activity in corn.

Treatment
each successive N applicatione6
Outcome
leaf nitrate content and enzyme activitye6
Direction
less effectivee6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“six corn ( Zea mays L.) hybrids grown under field conditions with supplemental N and irrigation”

✓ located verbatim in the source · abstract, characters 152–247 · show in text ↑
e6

“However, each successive N application was less effective in increasing and maintaining leaf nitrate content and enzyme activity”

✓ located verbatim in the source · abstract, characters 1110–1238 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

None of the corn hybrids maintained a high leaf blade nitrate concentration after silking.

Material
hybridse7
Outcome
maintain a high concentration of leaf blade nitrate after silkinge7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“six corn ( Zea mays L.) hybrids grown under field conditions with supplemental N and irrigation”

✓ located verbatim in the source · abstract, characters 152–247 · show in text ↑
e7

“None of the hybrids were able to maintain a high concentration of leaf blade nitrate after silking”

✓ located verbatim in the source · abstract, characters 1240–1338 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Hybrid No. 4 maintained a slightly higher leaf nitrate and enzyme activity level than all other hybrids during the post-anthesis stage.

Material
hybrid No. 4e1
Outcome
level of nitrate concentration and enzyme activitye1
Direction
slightly highere1
Comparator
all other hybridse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“six corn ( Zea mays L.) hybrids grown under field conditions with supplemental N and irrigation”

✓ located verbatim in the source · abstract, characters 152–247 · show in text ↑
e1

“Compared to all other hybrids, hybrid No. 4 maintained a slightly higher level of nitrate concentration and enzyme activity in the leaves”

✓ located verbatim in the source · abstract, characters 1405–1542 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

A minimal number (1 to 3) of samplings would be as effective as full season sampling in selecting corn plants or varieties with high grain yield or grain protein potential.

Outcome
minimal number (1 to 3) of samplingse1
Qualifier
in selecting individual plants or varietiese1
Comparator
full season samplings (12 or more dates)e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“six corn ( Zea mays L.) hybrids grown under field conditions with supplemental N and irrigation”

✓ located verbatim in the source · abstract, characters 152–247 · show in text ↑
e1

“a minimal number (1 to 3) of samplings would be as effective as the laborious full season samplings (12 or more dates) in selecting individual plants or varieties”

✓ located verbatim in the source · abstract, characters 2035–2197 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

The highest correlations between NRA and grain yield and grain protein in corn occurred during ear initiation and development.

Organ / stage
ear initiation and developmente1
Outcome
yields of grain and grain protein (kg N/ha)e1
Direction
higheste1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“six corn ( Zea mays L.) hybrids grown under field conditions with supplemental N and irrigation”

✓ located verbatim in the source · abstract, characters 152–247 · show in text ↑
e1

“The highest correlations between NRA and yields of grain and grain protein (kg N/ha) were obtained during the stages of ear initiation and development”

✓ located verbatim in the source · abstract, characters 1864–2014 · show in text ↑

nitrogen · quality: protein · Zea mays (maize)Positive and negative results

181 papers: 164 positive, 17 negative or no effect

163 papers in the same direction as this paper · 14 in the opposite direction · 3 reporting no difference from an alternative

  1. no effect
  2. no effect
  3. negative

177 papers more in this group

See all 181 papers →

nitrogen · yield · Zea mays (maize)Positive and negative results

2,185 papers: 1,906 positive, 279 negative or no effect

278 papers in the same direction as this paper · 1,906 in the opposite direction

  1. positive
  2. positive
  3. positive

2,181 papers more in this group

See all 2,185 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.