Abstract
Abstract: Knobs are blocks of heterochromatin present on chromosomes of maize (Zea mays L.) and its relatives that have effects on the frequency of genetic recombination, as well as on chromosome behavior. Knob heterozygosity and instability in six maize inbred lines and one Z. diploperennis Iltis Doebley line were investigated using the fluorescence in situ hybridization (FISH) technique with knob-associated tandem repeats (180 bp and 350 bp (TR-1)) as probes. Signals of seven heterozygous knobs containing 180-bp repeats and of one heterozygous knob containing TR-1 were captured in chromosomes of all materials tested according to the results of FISH, which demonstrates that the 180-bp repeat is the main contributor to knob heterozygosity compared with the TR-1 element. In addition, one target cell with two TR-1 signals on one homolog of chromosome 2L, which was different from the normal cells in the maize inbred line GB57, was observed, suggesting knob duplication and an instability phenomenon in the maize genome. (Managing editor: Wei WANG)
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Seven heterozygous knobs containing 180-bp repeats were detected in the chromosomes of all materials tested.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knobs are blocks of heterochromatin present on chromosomes of maize (Zea mays L.)”
e1“Signals of seven heterozygous knobs containing 180-bp repeats and of one heterozygous knob containing TR-1 were captured in chromosomes of all materials tested according to the results of FISH”
Claim · model-written summary
One heterozygous knob containing TR-1 was detected in the chromosomes of all materials tested.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knobs are blocks of heterochromatin present on chromosomes of maize (Zea mays L.)”
e1“Signals of seven heterozygous knobs containing 180-bp repeats and of one heterozygous knob containing TR-1 were captured in chromosomes of all materials tested according to the results of FISH”
Claim · model-written summary
The 180-bp repeat is the main contributor to knob heterozygosity compared with the TR-1 element.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knobs are blocks of heterochromatin present on chromosomes of maize (Zea mays L.)”
e1“which demonstrates that the 180-bp repeat is the main contributor to knob heterozygosity compared with the TR-1 element”
Claim · model-written summary
One target cell in maize inbred line GB57 had two TR-1 signals on one homolog of chromosome 2L, unlike normal cells.
- Material
- maize inbred line GB57e2
- Organ / stage
- target celle2
- Outcome
- two TR-1 signals on one homolog of chromosome 2Le2
- Comparator
- normal cellse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knobs are blocks of heterochromatin present on chromosomes of maize (Zea mays L.)”
e2“one target cell with two TR-1 signals on one homolog of chromosome 2L, which was different from the normal cells in the maize inbred line GB57, was observed”
Claim · model-written summary
The observed TR-1 signals suggest knob duplication and an instability phenomenon in the maize genome.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knobs are blocks of heterochromatin present on chromosomes of maize (Zea mays L.)”
e3“suggesting knob duplication and an instability phenomenon in the maize genome”